Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Action Pattern of Anti-Yeast Substance Originated from Rahnella aquatilis Strain AY2000

Rahnella aquatilis AY2000균 유래의 항 효모물질의 작용양상

  • Park, Hae-Ji (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Kang, Min-Jung (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Lee, Jong-Hwan (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Kim, Kwang-Hyeon (Department of Life Science and Biotechnology, Dong-Eui University)
  • 박혜지 (동의대학교 자연과학대학 생명응용학과) ;
  • 강민정 (동의대학교 자연과학대학 생명응용학과) ;
  • 이종환 (동의대학교 공과대학 생명공학과) ;
  • 김광현 (동의대학교 자연과학대학 생명응용학과)
  • Received : 2011.03.15
  • Accepted : 2011.05.27
  • Published : 2011.06.30
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Abstract

For reduction of side effects by anti-fungal agents, a less toxic anti-fungal substance or a synergistic substance with a new mechanism is needed. The anti-yeast substance (AYS) originated from Rahnella aquatilis strain AY2000 is like to be a heterogeneous protein. The AYS inhibited the growth of Candida albicans in culture broth, and AYS-treated cells were arrested in each phase during cell cycle. Among AYS-treated cells, the population of the cells belonging to sub-G1 phase was not increased during cell cycle. Therefore, AYS has rather yeaststatic than yeastcidal effect to C. albicans. Moreover, with combination of itraconazole or fluconazole, AYS had a synergistic anti-yeast activity against Saccharomyces cerevisiae based on the analysis of fractional inhibitory concentration index.

항 진균제의 인체에 대한 부작용을 완화시키는 방법은 부작용이 적은 새로운 항 진균제 개발이나 기존 항 진균제와 혼용하여 상승효과를 나타내는 물질이 필요하다. 이를 위해 Rahnella aquatilis AY2000가 생산하는 항 효모물질은 일종의 단백질성 고분자 물질이란 점에서 기존 항 진균제와 차이가 있다. 이 항 효모물질은 유도기나 대수증식 기에 있는 효모 생육을 억제시켰으며, cell cycle 분석에서 sub-G1기에 속하는 세포수의 증가 없이 세포를 arrest시켰다. 따라서 항 효모물질은 Candida albicans에 정균작용을 나타내었다. 또한 in vitro 실험에서 이 항 효모물질과 itraconazole이나 fluconazole을 병용한 후 fractional inhibitory concentration index 분석을 행한 결과 항 효모활성이 더욱 상승되었다. 결론적으로 이 항 효모물질은 정균 작용을 가질 뿐만 아니라 azole계의 항 진균제와 혼용하면 상승효과를 가짐으로 부작용이 적은 새로운 개념의 항 진균제로 개발할 가치가 있을 것이라고 생각된다.

Keywords

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