Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Effects of Astragali Radix Herbal Acupuncture Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity

황기 약침액의 사람 섬유아 세포에서 Collagen 생성과 Tyrosinage 활성에 미치는 영향

  • Kim, Jee-Hyun (Dept. of Acupuncture & Moxibution, Acupoint, Colleage of Oriental Medicine, Daegu Haany University) ;
  • Jung, Tae-Young (Je-Han Oriental Medical Academy) ;
  • Lee, Bong-Hyo (Dept. of Acupuncture & Moxibution, Acupoint, Colleage of Oriental Medicine, Daegu Haany University) ;
  • Lee, Yun-Kyu (Dept. of Acupuncture & Moxibution, Acupoint, Colleage of Oriental Medicine, Daegu Haany University) ;
  • Kim, Jae-Su (Dept. of Acupuncture & Moxibution, Acupoint, Colleage of Oriental Medicine, Daegu Haany University) ;
  • Lee, Kyung-Min (Dept. of Acupuncture & Moxibution, Acupoint, Colleage of Oriental Medicine, Daegu Haany University) ;
  • Lim, Seong-Chul (Dept. of Acupuncture & Moxibution, Acupoint, Colleage of Oriental Medicine, Daegu Haany University)
  • 김지현 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 정태영 (제한동의학술원) ;
  • 이봉효 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이윤규 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 김재수 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이경민 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구경혈학교실)
  • Received : 2011.04.26
  • Accepted : 2011.06.01
  • Published : 2011.06.20
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Abstract

목적 : 본 연구는 황기약침액의 collagen 대사과정과 tyrosinage 활성에 대한 효과를 관찰하여 항노화 약침소재로의 개발 가능성을 알아보고자 계획되었다. 방법 : 황기 약침액이 사람 정상 섬유아세포 HS68에 UVB 조사 후 type I procollagen의 생산량 회복과 collagenage 활성에 미치는 효과를 ELISA법을 이용하여 측정하였다. 황기약침액의 tyrosinage 활성도에 미치는 영향을 측정하였다. 결과 : 황기 약침액은 사람 섬유아세포 HS68 실험에서 UVB 조사로 감소된 type I procollagen생성을 회복시키고, 통계적으로 유의하게 collagenage 활성을 억제하는 것을 관찰하였고 tyrosinage 활성을 통계적으로 유의하게 억제하지만, L-DOPA 산화는 억제하는 경향이 있는 것을 관찰하였다. 결론 : 본 연구 결과에 의하면, 황기약침액은 collagenage 활성을 억제하고, tyrosinage 활성을 억제하므로 주름개선과 미백효과가 있어 미용약침 소재로 개발가능성이 있을 것으로 사료된다.

Keywords

References

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