The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Standardization of Eleutherococcus species and HPLC Method Validation for Quantitative Analysis

정량분석을 통한 Eleutherococcus species의 HPLC 분석법 검증과 표준화

  • Song, Mi-Kyung (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Mi-Yeon (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Ho-Cheol (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University)
  • 송미경 (경희대학교 한의과대학 본초학교실) ;
  • 김미연 (경희대학교 한의과대학 본초학교실) ;
  • 김호철 (경희대학교 한의과대학 본초학교실)
  • Received : 2010.12.09
  • Accepted : 2011.03.10
  • Published : 2011.03.30
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Abstract

Objective : For the standardization and quality control of eleutheroside E in Eleutherococcus species, HPLC analysis was performed and eleutherosdie E content was compared in 23 kinds of Eleutherococcus species collected from Korea and China. Methods : The content of eleutheroside E in stem bark of Eleutherococcus species collected from Korea and China were analyzed by HPLC. 0.5% phosphoric acid and acetonitrile was used as mobile solvent. Validation of HPLC analysis method was confirmed by analyzing specificity, linearity, precision and accuracy following ICH guideline. Results : Content of eleutheroside E was determined to be 1.0-1.6% and 0.5-0.8% in Korean and Chinese E. senticosus, respectively. Content of eleutheroside E in E. sessiliflorus was 0.7-1.1% and 0.2-0.4% respectively in Korean and Chinese origin. All calibration curves showed good linear regression. The method showed good precision and accuracy with intra-day and inter-day variations of 0.880-3.442% (RSD) and 0.606-3.328% (RSD), respectively, and average recovery was of 0.141-1.363% (RSD), for the eleutheroside E analyzed. Conclusion : These results might be used to establish a criterion of eleutheroside E in Eleutherococcus species.

Keywords

References

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