The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The Effects of Nelumbo nucifera on Utraviolet-B Irradiated human Keratinocytes

연잎, 연꽃, 연꽃 수술 추출물이 UVB 자외선 조사에 의한 각질형성세포의 보호 및 피부 노화 방지에 미치는 영향

  • Chang, Mun-Seog (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Ko, Eun-Bit (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Ho-Jin (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Ju-Sung (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Jin-Soo (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Jee, Sung-Won (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Hyu-Young (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Yeom, Myeong-Hoon (Skin Research Institute, R&D Center, AmorePacific Corporation) ;
  • Kim, Duck-Hee (Skin Research Institute, R&D Center, AmorePacific Corporation) ;
  • Kim, Han-Kon (Skin Research Institute, R&D Center, AmorePacific Corporation) ;
  • Park, Seong-Kyu (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
  • 장문석 (경희대학교 한의과대학 처방제형학교실) ;
  • 고은빛 (경희대학교 한의과대학 처방제형학교실) ;
  • 이호진 (경희대학교 한의과대학 처방제형학교실) ;
  • 김주성 (경희대학교 한의과대학 처방제형학교실) ;
  • 김진수 (경희대학교 한의과대학 처방제형학교실) ;
  • 지성원 (경희대학교 한의과대학 처방제형학교실) ;
  • 김휴영 (경희대학교 한의과대학 처방제형학교실) ;
  • 염명훈 ((주) 아모레퍼시픽 기술연구원 피부과학연구소) ;
  • 김덕희 ((주) 아모레퍼시픽 기술연구원 피부과학연구소) ;
  • 김한곤 ((주) 아모레퍼시픽 기술연구원 피부과학연구소) ;
  • 박성규 (경희대학교 한의과대학 처방제형학교실)
  • Received : 2011.05.06
  • Accepted : 2011.06.10
  • Published : 2011.06.30
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Abstract

Objectives : The purpose of this study was to investigate the anti-aging effects on cultured human keratinocytes with Nelumbo nucifera extracts. Methods : Each parts of leaves, flowers and stamen were extracted with water or 70% ethanol. These extracts were tested for cell viability on HaCaT cells (human keratinocyte line) by MTT assay. We investigated the effects of Ultraviolet-B (UVB) irradiation on cytotoxicity and lipid peroxidation in cultured skin keratinocytes. Results : The ethanol extract of Nelumbo nucifera flowers showed maximun cell viability as 111.39% in 30 ug/ml concentration. The water extracts of stamen, flowers, leaves showed cell viability as 107.12, 101.65, 101.46%, respectively. HaCaT keratinocytes were survived 63.06% at $20mJ/cm^2$ UVB irradiation. The cell membrane lipid peroxidation was measured by accumulation malondialdehyde (MDA). The levels of MDA were decreased by the ethanol extract of Nelumbo nucifera flowers and the water extracts of stamen. Conclusions : These finding suggest that the ethanol extract of Nelumbo nucifera flowers prevent anti-aging effects on cultured human keratinocytes during UVB irradiation.

Keywords

References

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