The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The anti-inflammatory effect of Achyranthes japonica on lipopolysaccharide-induced inflammatory activity in murine macrophages

LPS로 유도한 대식세포의 염증반응에서 우슬의 항염증 효과

  • Kim, Min-Sun (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Jeong, Jin-Soo (Dept. of Herbology, College of Oriental Medicine, Woosuk University) ;
  • Lee, Hye-Youn (Dept. of Herbology, College of Oriental Medicine, Woosuk University) ;
  • Ju, Young-Sung (Dept. of Herbology, College of Oriental Medicine, Woosuk University) ;
  • Bae, Gi-Sang (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Seo, Sang-Wan (ChungBuk Techno Park Bio center) ;
  • Cho, Il-Joo (Dept. of Beauty science, Kwangju Woman's Unversity) ;
  • Park, Sung-Joo (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Song, Ho-Joon (Dept. of Herbology, College of Oriental Medicine, Wonkwang University)
  • 김민선 (원광대학교 한의과대학 본초학교실) ;
  • 정진수 (우석대학교 한의과대학 본초학교실) ;
  • 이혜윤 (우석대학교 한의과대학 본초학교실) ;
  • 주영승 (우석대학교 한의과대학 본초학교실) ;
  • 배기상 (원광대학교 한의과대학 본초학교실) ;
  • 서상완 (충북테크노파크 바이오센터 전통의학지원팀) ;
  • 조일주 (광주여자대학교 미용과학과) ;
  • 박성주 (원광대학교 한의과대학 본초학교실) ;
  • 송호준 (원광대학교 한의과대학 본초학교실)
  • Received : 2011.05.10
  • Accepted : 2011.06.10
  • Published : 2011.06.30
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Abstract

Objectives : Achyranthes japonica (AJ) has been used as an anti-bacterial and anti-inflammatory agent. However, it is unclear that AJ could show the anti-inflammatory effects in macrophages. In this experiment, we studied whether AJ could inhibit the inflammatory responses in macrophages. Methods : To measure out the cytotoxicity of AJ, we performed the MTT assay. We evaluated the nitric oxide (NO) production, and cytokine production such as interleukin (IL)-1b, IL-6 and tumor necrosis factor (TNF)-a. We also investigated the cellular mechanims such as mitogen activated protein kinases (MAPK)s and nuclear factor kappa B (NF-kB). Results : AJ inhibited lipopolysaccharide (LPS)-induced NO production. AJ also inhibited production levels of IL-1b, IL-6 and TNF-a in LPS-stimulated macrophage. Finally, western blot analysis showed that AJ treatment inhibited the activation of p38 but not of extracellular signal-regulated kinase, c-jun NH2-terminal kinase and NF-kB. Conclusions : These results showed that AJ down-regulated the inflammatory response via p38 in macrophages, which suggest that AJ could be a candidate on treating inflammatory diseases.

Keywords

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