Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Identification of two cytopathogenic agents, Mycoplasma hyorhinis and mammalian orthoreovirus 3 based on modified particle associated nucleic acids PCR

  • Kim, Hye Kwon (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Moon, Hyoung Joon (Research Unit, Green Cross Veterinary Products) ;
  • Park, Seong Jun (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Rho, Se Mi (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Han, Jae Yeon (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Nguyen, Van Giap (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Park, Bong Kyun (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
  • Accepted : 2011.03.29
  • Published : 2011.06.30
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Abstract

Swine diseases could be caused by unrecognized or minor pathogens. In this study, two unknown cytopathogenic agents were isolated from swine, through cell culture. In order to identify these two cytopathogenic agent (designated CP129 and #2045-7), a particle associated nucleic acids PCR (PANPCR) from previous paper was used with simple modification. The cloning procedure was more specified in this study by adding cell control system. According to the modified PAN-PCR, two and four agentsspecific DNA sequences were obtained from CP129 and #2045-7, respectively, and they were identified as Mycoplasma (M.) hyorhinis and Mammalian orthoreovirus by nucleotide BLAST. Since M. hyorhinis (CP129) was filterable and non-visible by microscope, this unusual virus-like nature of M. hyorhinis (CP129) was discussed. Especially, the reovirus (#2045-7) was a serotype 3 and a triple reassortant among three serotypes of reoviruses. It was grouped with recently reported reoviruses from disease cases (swine, human and feline), based on the genetic analysis of L1 and S1 partial sequences. In conclusion, two unknown cytopathogenic agents were successfully identified using modified PAN-PCR with cell control system and they were characterized in this study.

Keywords

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