Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Quantification and genotyping of PCV2 DNA in the tissues of PCV2-infected conventional pigs with different clinical signs

  • Kim, Hye Kwon (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Luo, Yuzi (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Moon, Hyoung Joon (Research Unit, Green Cross Veterinary Products) ;
  • Park, Seong Jun (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Rho, Se Mi (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Han, Jae Yeon (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Nguyen, Van Giap (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Park, Bong Kyun (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
  • Accepted : 2010.12.24
  • Published : 2011.03.30
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Abstract

This study was focused on the genotyping and quantification of Porcine circovirus type 2 (PCV2) in thirty PCV2-positive pigs with different clinical symptoms (PCV2-infected without wasting, PCV2-infected with wasting, PCV2-infected with wasting and lymphoid depletion). The quantity of PCV2 DNA in diverse tissues was significantly differed among these groups. (One-way ANOVA test, p<0.001) Interestingly, PCV2-DNA load in tissues of PCV2-infected pigs without wasting and PCV2-infected pigs with wasting and lymphoid depletion were not significantly differed (p = 0.38), while they were all significantly higher when compared with PCV2-infected pigs with wasting-only. PCV2 DNA quantity in tissues was significantly higher in PCV2a and 2b co-infected pigs compared to the PCV2b only-infected pigs (Wilcoxon test, p = 0.039). The PCV2a and 2b co-infected pigs had increased wasting and lymphoid depletion rate but it was not statistically significant. Therefore, this cross-sectional study suggested that PCV2 DNA load in tissues was diverse by clinical and histological findings. Furthermore, co-infection of PCV2a and 2b affected to the PCV2 DNA load in tissues with increased rate of wasting and lymphoid depletion.

Keywords

References

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