Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
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The Inhibitory Potency of Hesperidin on Protein Kinase C Activity Using a Biochip

바이오칩을 이용한 Protein Kinase C의 활성에 대한 헤스페리딘의 저해 효과

  • Kang, Jung Ae (Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Rho, Jong Kook (Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Mi Hee (Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jung, Young Jin (Department of Food and Nutrition, Chungnam National University) ;
  • Park, Sang Hyun (Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 강정애 (한국원자력연구원 정읍방사선과학연구소 방사선생명공학연구부) ;
  • 노종국 (한국원자력연구원 정읍방사선과학연구소 방사선생명공학연구부) ;
  • 최미희 (한국원자력연구원 정읍방사선과학연구소 방사선생명공학연구부) ;
  • 정영진 (충남대학교 식품영양학과) ;
  • 박상현 (한국원자력연구원 정읍방사선과학연구소 방사선생명공학연구부)
  • Received : 2011.02.10
  • Accepted : 2011.02.28
  • Published : 2011.03.30

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Abstract

Protein kinases are the most important drug targets for the treatment of numerous diseases. The involvement of protein kinase C (PKC) in many biological processes such as development, memory, cell differentiation, and proliferation has been demonstrated. PKC is recognized as an important player in carcinogenesis. Thus, a variety of PKC inhibitors have been investigated. Among them, flavonoids have been demonstrated to affect the activity of many mammalian in vitro enzyme systems. The recent investigation was performed to evaluate the inhibitory effects of hesperidin, which is a flavonoid, on the proliferation and carcinogenesis of many cancers. In this study, an efficient kinase assay based on a biochip using radio-phosphorylation was established and performed for an examination of the inhibitory effects of hesperidin on PKC activity at different concentrations of 50, 200, 500 nM. It was found that hesperidin shows inhibitory potency on PKC, and that the biochip can be used to rapidly screen kinase inhibitors resulting in the therapeutic agents.

Keywords

Acknowledgement

Supported by : 교육과학기술부