한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제41권11호
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  • Pages.1540-1545
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  • 2012
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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야관문 에탄올 추출물의 자외선 조사에 의한 피부 광노화 억제 효과

Inhibitory Effects of Lespedeza cuneata Ethanol Extract on Ultraviolet-Induced Photo Aging

  • 김혜정 (경운대학교 임상병리학과) ;
  • 김길수 (경북대학교 수의과대학) ;
  • 김대익 ((재)대구테크노파크 한방산업지원센터)
  • Kim, Hye-Jeong (Dept. of Biomedical Laboratory Science, Kyungwoon University) ;
  • Kim, Kil-Soo (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Dae-Ik (Oriental Medicine Industry Support Center, Daegu Technopark)
  • 투고 : 2012.09.14
  • 심사 : 2012.10.22
  • 발행 : 2012.11.30
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초록

야관문 추출물의 UVB에 의한 피부노화 억제활성을 살펴보고자 야관문 추출물을 대상으로 피부 홍반도, 유수분 함량, 형태학 조직학적 검사, GSH, TBARS의 함량 그리고 XO, SOD, CAT 활성을 측정하였다. 피부 홍반도는 야관문 추출물 3% 도포군이 대조군에 비해 9.3% 홍반 감소 효과를 나타내었으며 유수분 함량은 야관문 추출물 3% 도포군이 대조군에 비해 각각 70%와 29% 유의한 증가를 보였다. 형태학적으로 야관문 추출물 도포한 군에서 피부의 주름이 현저하게 감소하였으며, 조직학적 검사에서도 표피 및 진피의 화농과 염증의 치료에 효과가 있음을 확인하였다. 야관문 추출물 3% 도포군에서 대조군에 비해 GSH는 유의하게 증가하였고 TBARS와 XO의 활성은 유의하게 감소하였으며, 항산화 효소인 SOD, CAT 활성은 유의한 증가를 나타내었다. 이에 UVB 조사로 노화가 유발된 hairless mouse에서 야관문 추출물의 도포는 노화된 피부에 염증반응을 약화시키며 유수분 함량을 증가시켜 피부장벽의 손상을 방지하며, 항산화효소의 활성을 증가시켜 노화억제 효과가 있는 것으로 나타났다. 야관문 추출물은 피부노화 예방 및 기능성 화장품 원료로서 사용 가능성을 제시하는 바이며 사용 농도는 3%가 효능적인 면에서 유익할 것으로 사료된다.

To investigate the inhibitory effects of Lespedeza cuneataextract (LC) on photo aging, skin aging was induced by UVB irradiation of hairless mice for 5 weeks. The skin erythema indices for the positive control (PC) and Lespedeza cuneata extract (LC-1%, 3%, and 5%) groups were lower than that of the control group. However, both lipid and water capacities of the PC and LC-3% groups were significantly higher than those of the control group. Skin wrinkles in the PC and LC-3% groups formed in a pattern of shallow furrows and thin crests. Skin TBARS contents and XO activity in the LC group were lower than those of the control group. The activities of GSH, SOD, and CAT in the LC-3% group were significant higher than those of the control group. Therefore, Lespedeza cuneata extract could be an effective natural herbal material for the inhibition of skin aging in hairless mice skin.

키워드

참고문헌

  1. Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, Fukunaga M, Tsuru K, Horikawa T. 2003. UV-induced skin damage. Toxicology 189: 21-39. https://doi.org/10.1016/S0300-483X(03)00150-1
  2. Lim AK, Jung YJ, Kim KS, Kim YH, Kwak JH, Hong JH, Kim HY, Kim DI. 2010. Skin UVB photo aging effect from extract of fermented Reynoutria elliptica. J Korean Soc Food Sci Nutr 39: 369-375. https://doi.org/10.3746/jkfn.2010.39.3.369
  3. Darr D, Fridovich I. 1994. Free radical in cutaneous biology. J Invest Dermatol 102: 671-675. https://doi.org/10.1111/1523-1747.ep12374036
  4. Sies H. 1986. Biochemistry of oxidative stress. Angewandte Chem 25: 1058-1071. https://doi.org/10.1002/anie.198610581
  5. Niwa Y, Kasama T, Kawai S, Komura J, Sakane T, Kanoh T, Miyachi Y. 1988. The effect of aging on cutaneous lipid peroxide levels and superoxide dismutase activity in guinea pigs and patients with burns. Life Sci 42: 351-356. https://doi.org/10.1016/0024-3205(88)90072-0
  6. Cho SH. 2009. Inhibitory effect of PME88 melonSOD on the ultraviolet-induced photo-aging. J Korean Soc Food Sci Nutr 38: 401-408. https://doi.org/10.3746/jkfn.2009.38.4.401
  7. Cao G, Alessio HM, Cutler RG. 1993. Oxygen-radical absorbance capacity assay for antioxidants. Free Radical Biol Med 14: 303-311. https://doi.org/10.1016/0891-5849(93)90027-R
  8. Lee HJ, Lim GN, Park MA, Park SN. 2011. Antibacterial and antioxidative activity of Lespedeza cuneata G. Don extracts. Korean J Microbiol Biotechnol 39: 63-69.
  9. Shigemori H, Sakai N, Miyoshi E, Shizuri Y, Yamamura S. 1990. Bioactive substances from Lespedeza cuneata L.G. Don and their biological activities. Tetrahedron 46: 383-394. https://doi.org/10.1016/S0040-4020(01)85423-5
  10. Numata A, Hokimoto K, Yamaguchi H. 1980. C-Glycoylflavones in Lespedeza cuneata. Chem Pharm Bull 28: 964-965. https://doi.org/10.1248/cpb.28.964
  11. Kim SJ, Kim DW. 2007. Antioxidative activity of hot water and ethanol extracts of Lespedeza cuneata seeds. Korean J Food Preserv 14: 332-335.
  12. Ellman GL. 1959. Tissue sulfhydryl group. Arch Biochem Biophys 82: 70-77. https://doi.org/10.1016/0003-9861(59)90090-6
  13. Ohkawa H, Ohishi N, Yake K. 1979. Acid reaction. Anal Biochem 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  14. Stripe F, Della CE. 1969. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) oxidase (type O). J Biol Chem 244: 3855-3863.
  15. Martin JP Jr, Dailey M, Sugarman E. 1987. Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255: 329-336. https://doi.org/10.1016/0003-9861(87)90400-0
  16. Aebi H. 1974. Catalase. In Methods of Enzymatic Analysis. Bergmeyer HU, ed. Academic press, New York, NY, USA. Vol 2, p 673.
  17. Soter NA. 1990. Acute effects of ultraviolet radiation on the skin. Semin Dermatol 9: 11-15.
  18. Kwon MH, Min KJ, Kim YC. 2009. Inhibitory effects of Peonia japonica water extract on skin aging ( I ) - focussed on alleviative effects of inflammation and skin barrier damage - . J Environ Toxicol 24: 159-167.
  19. Chiba K, Kawakami K, Sone T, Onoue M. 2003. Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin. Skin Pharmacol Appl Skin Physiol 16: 242-251. https://doi.org/10.1159/000070847
  20. Fisher GJ, Kang S, Varani J, Bata-Csorgo Z, Wan Y, Datta S, Voorhees JJ. 2002. Mechanisms of photoaging and chronological skin aging. Arch Dermatol 138: 1462-1470. https://doi.org/10.1001/archderm.138.11.1462
  21. Kligman LH, Crosby MJ, Higley H. 1993. The UVB-irradiated hairless mouse as a model for the evaluation of injectable collagen implant. J Dermatol Treat 4: 27-31. https://doi.org/10.3109/09546639309088233
  22. Kaneto H, Fujii J, Suzuki K, Kasai H, Kawamori R, Kamada T, Taniguchi N. 1994. DNA cleavage induced by glycation of Cu, Zn-superoxide dismutase. Biochemical J 304: 219-225. https://doi.org/10.1042/bj3040219
  23. Im MJ, Manson PN, Bulkley GB, Hoopes JE. 1985. Effects of superoxide dismutase and allopurinol on the survival of acute island skin flaps. Ann Surg 201: 357-359. https://doi.org/10.1097/00000658-198503000-00018
  24. Manson PN, Anthenelli RM, Im MJ, Bulkley GB, Hoopes JE. 1983. The role of oxygen-free radicals in ischemic tissue injury in island skin flaps. Ann Surgery 198: 87-90. https://doi.org/10.1097/00000658-198307000-00017
  25. Deisseroth A, Dounce AL. 1970. Catalase: Physical and chemical properties, mechanism of catalysis, and physiological role. Physiol Rev 50: 319-375.
  26. Eun JS. 2011. Effect of Lespedeza cuneata G. Don on the activity of murine immune cells. Korean J Oriental Physiology & Pathology 25: 837-842.
  27. Kim BY. 2010. Studies on antioxidant activity of extracts from Lespedeza cuneata and development of fermented products prepared from Lespedeza cuneata. MS Thesis. Daegu Haany University, Gyeongbuk, Korea. p 41.

피인용 문헌

  1. Biological activities of the legume crops Melilotus officinalis and Lespedeza capitata for skin care and pharmaceutical applications vol.96, 2017, https://doi.org/10.1016/j.indcrop.2016.11.047
  2. Effects of Lespedeza Caneata (LC) Extracts on Atopic Dermatitis in DNCB-Induced Mice vol.7, pp.4, 2016, https://doi.org/10.15207/JKCS.2016.7.4.067
  3. Improving the efficacy of Lespedeza cuneata ethanol extract on ultraviolet-induced photoaging vol.21, pp.2, 2014, https://doi.org/10.11002/kjfp.2014.21.2.264
  4. Regeneration Effects of Lespedeza cuneata Ethanol Extract on Experimental Open Wound in Rat vol.43, pp.4, 2014, https://doi.org/10.3746/jkfn.2014.43.4.516
  5. Protective Effects of Pyrus pyrifolia NAKAI Leaf Extracts on UVB-induced Toxicity in Human Dermal Fibroblasts vol.42, pp.1, 2016, https://doi.org/10.15230/SCSK.2016.42.1.87
  6. Wound Healing Effects of Lespedeza cuneata Extract vol.43, pp.3, 2014, https://doi.org/10.3746/jkfn.2014.43.3.374
  7. Physiological activities of Lespedeza cuneata extracts vol.21, pp.6, 2014, https://doi.org/10.11002/kjfp.2014.21.6.844
  8. 야관문(Lespedeza Caneata) Ethanol 추출물이 알코올 투여한 생쥐의 간장에 미치는 영향 vol.17, pp.10, 2012, https://doi.org/10.5762/kais.2016.17.10.432