The Journal of Korean Academy of Prosthodontics (대한치과보철학회지)

The Korean Academy of Prosthodonitics (대한치과보철학회)
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A study of mesenchymal stem cell proliferation and surface characteristics of the titanium discs coated with MS275/PLGA by an electrospray

Electrospray법을 이용한 MS275/PLGA submicron 코팅 티타늄에서의 표면변화와 간엽줄기세포증식에 관한 연구

  • Yoo, Soo-Yeon (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Kim, Seong-Kyun (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Heo, Seong-Joo (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Koak, Jai-Young (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Lee, Joo-Hee (Department of Prosthodontics, Seoul Asan Medical Center, Ulsan University) ;
  • Park, Yoon-Kyung (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Kim, Ena (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University)
  • 유수연 (서울대학교 치의학대학원 치과보철학교실) ;
  • 김성균 (서울대학교 치의학대학원 치과보철학교실) ;
  • 허성주 (서울대학교 치의학대학원 치과보철학교실) ;
  • 곽재영 (서울대학교 치의학대학원 치과보철학교실) ;
  • 이주희 (울산대학교 서울아산병원 치과보철과) ;
  • 박윤경 (서울대학교 치의학대학원 치과보철학교실) ;
  • 김에나 (서울대학교 치의학대학원 치과보철학교실)
  • Received : 2012.09.17
  • Accepted : 2012.10.16
  • Published : 2012.10.31
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Abstract

Purpose: This study was conducted to identify the surface characteristics of titanium discs coated with MS275/PLGA by electrospray and which is effective to mesenchymal stem cell proliferation. Materials and methods: We used anodized surface coated with PLGA as a control group and anodized surface coated with MS275 $0.5{\mu}M$, $1{\mu}M$, $1.5{\mu}M$ as test groups. To examine that the coating particles are nanometer sized, FE-SEM was used and AFM was utilized to determine the difference of coating surface roughness. We checked the mesenchymal stem cell proliferation by using MTT assay on $1^{st}$, $4^{th}$, $7^{th}$ days. Results: There was no significant difference between control groups and test groups in AFM results (P>.05). In MTT assay results, mesenchymal stem cell proliferation was increased with time, at $7^{th}$ day, cell viability on discs coated with $1.5{\mu}M$ MS275 was significantly higher than control group (P<.05). As SEM showed, the number of cells on all discs was increased and the morphology of cell attachment was also wider and closer with time. Conclusion: Titanium surface coated with MS275/PLGA showed significantly higher cell proliferation and the more density of MS275 was dispersed on titanium discs, the faster cells grew.

연구 목적: 본 실험은 MS275와 PLGA (poly lactic-co-glycolic acid) 의 복합체를 submicron 크기로 티타늄 디스크 표면에 코팅하여, 표면의 변화를 알아보고 생물학적으로 간엽 줄기세포 활성에 어떠한 영향을 미치는지 조사하기 위해 시행되었다. 연구 재료 및 방법:양극산화 디스크에 electrospray 코팅법을 이용하여PLGA를 분사한 것을 대조군으로 설정하고, MS275를 $0.5{\mu}M$, $1{\mu}M$, $1.5{\mu}M$ 농도별로 코팅한 것을 실험군으로 하였다. 티타늄 디스크 표면에 분사된 복합체가 submicron입자 크기로 이루어졌는지SEM을 통해 확인하였으며, MS275로 코팅한 디스크와 양극산화 디스크의 거칠기 차이를 확인하기 위해 AFM으로 관찰하였다. 디스크 위에 간엽줄기세포 배양 후 1, 4, 7일에 세포증식 양상을 SEM과 MTT 검사를 통해 확인하였다. 결과: AFM (atomic force microscope) 결과 대조군과 실험군에서 거칠기의 유의할만한 차이가 없었다(P>.05). MTT 결과 1, 4, 7일 시간이 지남에 따라 세포 증식이 활발해졌으며 세포배양 7일에 $0.5{\mu}M-1.5{\mu}M$ MS275 농도 안에서, MS275의 농도가 커짐에 따라 세포의 활성도가 높아짐이 유의할 수준으로 확인되었다(P<.05). 세포 부착을 SEM으로 확인한 결과, 세포의 부착 수는 시간이 갈수록 증가하고 부착 형태 역시 돌기가 크고 넓어지며, 표면과 긴밀함 접촉이 증가하였다. 결론: FE-SEM과 MTT 결과 MS275/PLGA 복합체로 표면 처리된 타이타늄 표면에서 세포가 초기에 (7일내) 빠르게 증식하였다. 또한 복합체 처리군의 농도가 증가할수록 높은 세포 성장 수치를 보였다.

Keywords

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