Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Auxin Affects on Production of Adventitious Roots and Secondary Metabolites in Echinacea angustifolia

오옥신 처리가 에키네시아의 부정근 및 생리활성물질 생산에 미치는 영향

  • Jang, Young Se (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Cui, Hai Yan (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Lee, Eun Jung (Cheongsol Co. Ltd., Industry Academic Cooperation Foundation Agribusiness Incubator Center for Development of Advanced Horticultural Technology(205), Chungbuk National University) ;
  • Kim, Hae Won (Department of Chemical engineering Kyungil University) ;
  • Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
  • 장영세 (충북대학교 첨단원예기술개발연구센터) ;
  • 최해연 (충북대학교 첨단원예기술개발연구센터) ;
  • 이은정 (농업회사법인 청솔 바이오텍) ;
  • 김해원 (경일대학교 공과대학 화학공학과) ;
  • 백기엽 (충북대학교 첨단원예기술개발연구센터)
  • Received : 2012.10.16
  • Accepted : 2012.11.28
  • Published : 2012.12.30
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Abstract

The production of adventitious roots derived from root explant of Echinacea angustifolia and its secondary metabolite content were assessed in different types and levels of auxin. The induction of adventitious roots from root explant cultured in Murashige and Skoog solid medium supplemented with 1.0 mg/L indole -3-butyric acid (IBA) attained highest as 20.87 mg fresh weight and 3.07 mg dry weight per culture but root suspension culture at the same concentration of IBA enhanced biomass production as 3.07 g fresh weight and 0.38 g per culture after 4 weeks in culture. 3.0 mg/L ${\alpha}$-naphthalene acetic acid (NAA) treatment had similar effect on root biomass production as 3.07 g fresh weight and 0.38 g per culture with liquid suspension culture, whereas adventitious roots exposed to over 3.0-5.0 mg/L IBA or 5.0 mg/L NAA were less responsive by reducing the number of adventitious roots and/or changing root morphology such as short and thick. The content of secondary metabolites such as phenolic, flavonoids and total caffeic acid in adventitious roots cultured on MS medium supplemented with 1.0 mg/L IBA were attained highest as 27.20, 9.60. 10.67 mg/g dry weight, respectively. Overall, the best production of root biomass and secondary metabolites were given by 1.0 mg/L IBA.

Keywords

References

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