대한본초학회지 (The Korea Journal of Herbology)

  • 제27권2호
  • /
  • Pages.53-59
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  • 2012
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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In vitro 동물세포에서 GGEx18의 ethyl acetate 분획물에 의한 지방산 β-산화효소 유전자 발현의 조절

Ethyl acetate fraction of GGEx18 modulates fatty acid β-oxidizing enzymes

  • 주병수 (동의대학교 한의과대학 방제학교실) ;
  • 이희영 (동의대학교 한의과대학 방제학교실) ;
  • 이혜림 (동의대학교 한의과대학 방제학교실) ;
  • 윤미정 (목원대학교 바이오건강학부) ;
  • 서부일 (대구한의대학교 한의과대학 본초학교실) ;
  • 김범회 (동의대학교 해부학교실과 한의학연구소) ;
  • 신순식 (동의대학교 한의과대학 방제학교실)
  • Joo, Byung-Soo (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University) ;
  • Lee, Hee-Young (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University) ;
  • Lee, Hye-Rim (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University) ;
  • Yoon, Mi-Chung (Dept. of Life Sciences, Mok-Won University) ;
  • Seo, Bu-Il (Dept. of Herbology, College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Beom-Hoi (Dept. of Anatomy, College of Oriental Medicine & Research Institute of Oriental Medicine, Dong-Eui University) ;
  • Shin, Soon-Shik (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University)
  • 투고 : 2012.02.13
  • 심사 : 2012.03.16
  • 발행 : 2012.03.30
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초록

Objectives : This study was undertaken to investigate the effects of the GGEx18 ethyl acetate fraction (EF) on lipid accumulation and gene expression of fatty acid-oxidizing enzymes using 3T3-L1 adipocytes, C2C12 skeletal muscle cells, and NMu2Li liver cells. Methods : PPAR${\alpha}$, AMPK and UCPs transactivation was examined in NMu2Li hepatocytes, C2C12 myocytes, and 3T3-L1 preadipocytes using transient transfection assays. Results : 1. Compared with control, EF significantly increased the mRNA expression of VLCAD in 3T3-L1 adipocytes. 2. Compared with control, EF (0.1 ${\mu}g/ml$) significantly inhibited lipid accumulation in 3T3-L1 adipocytes. 3. EF significantly increased the mRNA expression of AMPK${\alpha}$1, AMPK${\alpha}$2 and PPAR${\alpha}$ in C2C12 skeletal muscle cells compared with control. 4. EF significantly increased the mRNA expression of genes involved in fatty acid ${\beta}$-oxidation, such as thiolase, MCAD, and CPT-1 in C2C12 skeletal muscle cells compared with control. 5. EF significantly increased the mRNA expression of UCP2 involved in energy expenditure in C2C12 skeletal muscle cells compared with control. 6. Compared with control, EF (10 ${\mu}g/ml$) significantly inhibited lipid accumulation in C2C12 skeletal muscle cells. 7. EF (10 ${\mu}g/ml$) significantly increased the mRNA expression of ACOX, HD, VLCAD and MCAD in NMu2Li liver cells compared with control. Conclusions : These results suggest that EF may prevent obesity by increasing the mRNA expression of mitochondrial fatty acid ${\beta}$-oxidizing enzymes in 3T3-L1 adipocytes, by not only regulating the fatty acid oxidation through activation of AMPK and PPAR${\alpha}$, but also increasing the UCP2 mRNA expression in C2C12 skeletal muscle cells, and by stimulating the mRNA expression of fatty acid-oxidizing enzymes in NMu2Li liver cells.

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