Toxicological Research

  • 제28권1호
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  • Pages.11-18
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  • 2012
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
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Mouse Single Oral Dose Toxicity Test of Bupleuri Radix Aqueous Extracts

  • Kim, Kyung-Hu (College of Oriental Medicine, Daegu Haany University) ;
  • Gam, Cheol-Ou (Department of Oriental Medicine, Graduate School, Dong Eui University) ;
  • Choi, Seong-Hun (College of Oriental Medicine, Daegu Haany University) ;
  • Ku, Sae-Kwang (College of Oriental Medicine, Daegu Haany University)
  • 투고 : 2012.02.08
  • 심사 : 2012.03.21
  • 발행 : 2012.03.31
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초록

The aim of this study was to evaluate the single oral dose toxicity of Bupleuri Radix (BR) aqueous extracts, it has been traditionally used as anti-inflammatory agent, in male and female mice. BR extracts (yield = 16.52%) was administered to female and male ICR mice as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines. Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 14 principal organs were examined. As the results, no BR extracts treatment related mortalities, clinical signs, changes on the body and organ weights, gross and histopathological observations against 14 principal organs were detected up to 2,000 mg/kg in both female and male mice, except for soft feces and related body weight decrease detected in male mice treated with 2,000 mg/kg. Therefore, $LD_{50}$ (50% lethal dose) and approximate LD of BR aqueous extracts after single oral treatment in female and male mice were considered over 2000 mg/kg, respectively. Although it was also observed that the possibilities of digestive disorders, like soft feces when administered over 2,000 mg/kg of BR extracts in the present study, these possibilities of digestive disorders can be disregard in clinical use because they are transient in the highest dosages male only.

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참고문헌

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피인용 문헌

  1. A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications vol.55, pp.1, 2017, https://doi.org/10.1080/13880209.2016.1262433
  2. Current Status of Herbal Medicines in Chronic Liver Disease Therapy: The Biological Effects, Molecular Targets and Future Prospects vol.16, pp.12, 2015, https://doi.org/10.3390/ijms161226126