Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-hyperlipidemic Effect of Polyphenol Extract (SeapolynolTM) and Dieckol Isolated from Ecklonia cava in in vivo and in vitro Models

  • Yeo, A-Reum (Department of Microbiology, College of Medicine, Konyang University) ;
  • Lee, Jung-Lim (Department of Microbiology, College of Medicine, Konyang University) ;
  • Tae, In-Hwan (Department of Microbiology, College of Medicine, Konyang University) ;
  • Park, Seok-Rae (Department of Microbiology, College of Medicine, Konyang University) ;
  • Cho, Young-Ho (Department of Pharmaceutics & Biotechnology, Medical Engineering College, Konyang University) ;
  • Lee, Bong-Ho (Division of Applied Chemistry and Biotechnology, College of Engineering, Hanbat National University) ;
  • Shin, Hyeon-Cheol (Botamedi Inc.) ;
  • Kim, Seong-Ho (Botamedi Inc.) ;
  • Yoo, Yung-Choon (Department of Microbiology, College of Medicine, Konyang University)
  • Received : 2012.01.16
  • Accepted : 2012.02.28
  • Published : 2012.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The inhibitory effect of polyphenol extracts (Seapolynol$^{TM}$, SPN) of the marine brown algae Ecklonia cava and dieckol, a major component of SPN, on hyperlipidemia was investigated in ICR mice fed a high-fat diet (HFD) for five weeks. For analysis of the anti-hyperlipidemic effects of SPN and dieckol, these two agents were given orally on a daily basis to HFD-fed mice for four weeks, starting one week after the beginning of HFD feeding. Groups administered with SPN as well as dieckol showed lower body weight gains than the HFD only group. Administration of SPN and dieckol also resulted in a significant reduction of the level of total cholesterol (TCHO), triglyceride (TG), and low-density lipoprotein (LDL) cholesterol in the serum of HFD-fed mice. In Oil Red O staining using 3T3-L1 preadipocytes, it was shown that both SPN and dieckol markedly inhibited lipid accumulation of 3T3-L1 cells. Furthermore, SPN and dieckol (50 ${\mu}g$/mL) significantly inhibited 3-hydroxyl-methyl glutaryl coenzyme A (HMGCoA) reductase activity in vitro. Taken together, these results suggest that polyphenols of Ecklonia cava (SPN) and dieckol reduce body weight gain and fat accumulation in HFD-induced obese mice, and that their hypolipidemic effect is related to the inhibition of adipogenesis of adipocytes and HMGCoA reductase activity.

Keywords

References

  1. Frishman WH. 1998. Biologic markers as predictors of cardiovascular disease. Am J Med 104: 18S-27S. https://doi.org/10.1016/S0002-9343(98)00184-3
  2. Stone NJ. 1996. Lipid management: current diet and drug treatment options. Am J Med 101: 40S-49S. https://doi.org/10.1016/S0002-9343(96)00137-4
  3. Bhatnagar D. 1998. Lipid-lowering drugs in the management of hyperlipidemia. Pharmacol Therapeut 79: 205-230. https://doi.org/10.1016/S0163-7258(98)00018-7
  4. Taskin E, Ozturuk M, Kurt O. 2001. Turkiye'nin yararlanilabilir denizel alg potansiyeli. XI. Ulusal Su Urunleri Sempozyumu. p 225-232.
  5. Joe MJ, Kim SN, Choi HY, Shin WS, Park GM, Kang DW, Kim YK. 2006. The inhibitory effects of eckol and dieckol from Ecklonia stolonifera on the expression of matrix metalloproteinase-1 in human dermal fibroblast. Biol Pharm Bull 29: 1735-1739. https://doi.org/10.1248/bpb.29.1735
  6. Heo SJ, Kim JP, Jung WK, Lee NH, Kang HS, Jun EM, Park SH, Kang SM, Lee YJ, Park PJ, Jeon YJ. 2008. Identification of chemical structure and free radical scavenging activity of diphlorethohydroxycarmalol isolated from a brown alga, Ishige okamurae. J Microbiol Biotechnol 18: 676-681.
  7. Kang K, Park Y, Hwang HJ, Kim SH, Lee JG, Shin HC. 2003. Antioxidative properties of brown algae polyphenolics and their perspectives as chemopreventive agents against vascular risk factors. Arch Pharm Res 26: 286-293. https://doi.org/10.1007/BF02976957
  8. Nagayama K, Iwamura Y, Shibata T, Hirayama I, Nakamura T. 2002. Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. J Antimicrob Chemother 50: 889-893. https://doi.org/10.1093/jac/dkf222
  9. Shin HC, Hwang HJ, Kang KJ, Lee BH. 2006. An antioxidative and antiinflammatory agent for potential treatment of osteoarthritis from Ecklonia cava. Arch Pharm Res 29: 165-171. https://doi.org/10.1007/BF02974279
  10. Fukuyama Y, Kodama M, Miura I, Kinzyo Z, Mori H, Nakayama Y, Takahashi M. 1989. Anti-plasmin inhibitor. V. Structures of novel dimeric eckols isolated from the brown alga Ecklonia kurome OKAMURA. Chem Pharm Bull 37: 2438-2440. https://doi.org/10.1248/cpb.37.2438
  11. Jung HA, Hyun SK, Kim HR, Choi JS. 2006. Angiotensinconverting enzyme I inhibitory activity of phlorotannins from Ecklonia stolonifera. Fisheries Sci 72: 1292-1299. https://doi.org/10.1111/j.1444-2906.2006.01288.x
  12. Artan M, Li Y, Karadeniz F, Lee SH, Kim MM, Kim SK. 2008. Anti-HIV-1 activity of phloroglucinol derivative, 6,6'-bieckol, from Ecklonia cava. Bioorg Med Chem 16: 7921-7926. https://doi.org/10.1016/j.bmc.2008.07.078
  13. Hwang HJ, Chen T, Nines RG, Shin HC, Stoner GD. 2006. Photochemoprevention of UVB-induced skin carcinogenesis in SKH-1 mice by brown algae polyphenols. Int J Cancer 119: 2742-2749. https://doi.org/10.1002/ijc.22147
  14. Iwai K. 2008. Antidiabetic and antioxidant effects of polyphenols in brown alga Ecklonia stolonifera in genetically diabetic KK-A(y) mice. Plant Foods Hum Nutr 63: 163-169. https://doi.org/10.1007/s11130-008-0098-4
  15. Lee MH, Lee KB, Oh SM, Lee BH, Chee HY. 2010. Antifungal activities of dieckol isolated from the marine brown alga Ecklonia cava against Trichophyton rubrum. J Korean Soc Appl Biol Chem 53: 504-507. https://doi.org/10.3839/jksabc.2010.076
  16. Xie W, Wang W, Su H, Xing D, Cai G, Du L. 2007. Hypolipidemic mechanisms of Ananas comosus L. leaves in mice: different from fibrate but similar to statins. J Pharmacol Sci 103: 267-274. https://doi.org/10.1254/jphs.FP0061244
  17. Ji Z, Mei FC, Cheng X. 2010. Epac, not PKA catalytic subunit, is required for 3T3-L1 preadipocyte differentiation. Front Biosci 2: 392-398.
  18. Kim CK, Kim M, Oh SD, Lee SM, Sun B, Choi GS, Kim SK, Bae H, Kang C, Min BI. 2011. Effects of Atractylodes macrocephala Koidzumi rhizome on 3T3-L1 adipogenesis and an animal model of obesity. J Ethnopharmacol 137: 396-402. https://doi.org/10.1016/j.jep.2011.05.036
  19. Meier U, Gressner AM. 2004. Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, and resistin. Clin Chem 50: 1511-1525. https://doi.org/10.1373/clinchem.2004.032482
  20. Drevon CA. 2005. Fatty acids and expression of adipokines. Biochim Biophys Acta 1740: 287-292. https://doi.org/10.1016/j.bbadis.2004.11.019
  21. Wissler RW. 1992. Theories and new horizons in the pathogenesis of atherosclerosis and the mechanisms of clinical effects. Arch Pathol Lab Med 116: 1281-1291.
  22. Castelli WP. 1992. Epidemiology of triglycerides: a review from Framingham. J Cardiol 70: H3-H9. https://doi.org/10.1016/0002-9149(92)91083-G

Cited by

  1. Do marine algal polyphenols have antidiabetic, antihyperlipidemic or anti-inflammatory effects in humans? A systematic review 2018, https://doi.org/10.1080/10408398.2017.1301876
  2. Identification of microRNAs involved in the modulation of pro-angiogenic factors in atherosclerosis by a polyphenol-rich extract from propolis vol.557, 2014, https://doi.org/10.1016/j.abb.2014.04.009
  3. Anti-atherogenic properties of date vs. pomegranate polyphenols: the benefits of the combination vol.6, pp.5, 2015, https://doi.org/10.1039/C4FO00998C
  4. Antioxidant Activity of Marine Algal Polyphenolic Compounds: A Mechanistic Approach vol.19, pp.7, 2016, https://doi.org/10.1089/jmf.2016.3706
  5. An emerging trend in functional foods for the prevention of cardiovascular disease and diabetes: Marine algal polyphenols 2018, https://doi.org/10.1080/10408398.2016.1259209
  6. Effects of Myelophycus Simplex Papenfuss Methanol Extract on Adipocyte Differentiation and Adipogenesis in 3T3-L1 Preadipocytes vol.25, pp.1, 2015, https://doi.org/10.5352/JLS.2015.25.1.62
  7. Clinical Trial of the Hypolipidemic Effects of a Brown Alga Ecklonia cava Extract in Patients with Hypercholesterolemia vol.11, pp.7, 2015, https://doi.org/10.3923/ijp.2015.798.805
  8. Seapolynol Extracted from Ecklonia cava Inhibits Adipocyte Differentiation in Vitro and Decreases Fat Accumulation in Vivo vol.20, pp.12, 2015, https://doi.org/10.3390/molecules201219796
  9. Screening test for Dendropanax morbifera Leveille extracts: in vitro comparison to ox-LDL-induced lipid accumulation, ethanol-induced fatty liver and HMG-CoA reductase inhibition vol.61, pp.1, 2018, https://doi.org/10.3839/jabc.2018.001
  10. Safety of Ecklonia cava phlorotannins as a novel food pursuant to Regulation ( EC ) No 258/97 vol.15, pp.10, 2012, https://doi.org/10.2903/j.efsa.2017.5003
  11. Study protocol for a double-blind randomised controlled trial investigating the impact of 12 weeks supplementation with a Fucus vesiculosus extract on cholesterol levels in adults with elevated fast vol.8, pp.12, 2012, https://doi.org/10.1136/bmjopen-2018-022195
  12. Effect of Food Preparations on In Vitro Bioactivities and Chemical Components of Fucus vesiculosus vol.9, pp.7, 2020, https://doi.org/10.3390/foods9070955
  13. Seaweed Phenolics: From Extraction to Applications vol.18, pp.8, 2020, https://doi.org/10.3390/md18080384
  14. Brown Algae Potential as a Functional Food against Hypercholesterolemia: Review vol.10, pp.2, 2012, https://doi.org/10.3390/foods10020234
  15. Insights into phenolic compounds from microalgae: structural variety and complex beneficial activities from health to nutraceutics vol.41, pp.2, 2012, https://doi.org/10.1080/07388551.2021.1874284
  16. Acanthaster planci Inhibits PCSK9 and Lowers Cholesterol Levels in Rats vol.26, pp.16, 2021, https://doi.org/10.3390/molecules26165094
  17. Dieckol: a brown algal phlorotannin with biological potential vol.142, pp.None, 2012, https://doi.org/10.1016/j.biopha.2021.111988
  18. Therapeutic Potential of Seaweed-Derived Bioactive Compounds for Cardiovascular Disease Treatment vol.12, pp.3, 2012, https://doi.org/10.3390/app12031025