Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

In vivo Osteogenesis of Cultured Human Periosteal-derived Cells and Polydioxanone/Pluronic F127 Scaffold

인간 골막기원세포와 Polydioxanone/Pluronic F127 담체를 이용한 골형성

  • Park, Bong-Wook (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine, Institute of Health Sciences, Gyeongsang National University, Biomedical Center (BK21)) ;
  • Lee, Jin-Ho (Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University) ;
  • Oh, Se-Heang (Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Sang-June (Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University) ;
  • Hah, Young-Sool (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Jeon, Ryoung-Hoon (Department of Obstetrics/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Maeng, Geun-Ho (Department of Obstetrics/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Rho, Gyu-Jin (Department of Obstetrics/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Jong-Ryoul (Maxillofacial Center, On Hospital) ;
  • Byun, June-Ho (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine, Institute of Health Sciences, Gyeongsang National University, Biomedical Center (BK21))
  • 박봉욱 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원, 의생명과학사업단) ;
  • 이진호 (한남대학교 생명나노과학대학 신소재공학과) ;
  • 오세행 (한남대학교 생명나노과학대학 신소재공학과) ;
  • 김상준 (한남대학교 생명나노과학대학 신소재공학과) ;
  • 하영술 (경상대학교병원 임상의학연구소) ;
  • 전령훈 (경상대학교 수의과대학 수의산과) ;
  • 맹건호 (경상대학교 수의과대학 수의산과) ;
  • 노규진 (경상대학교 수의과대학 수의산과) ;
  • 김종렬 (온 종합병원 턱얼굴센터) ;
  • 변준호 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원, 의생명과학사업단)
  • Received : 2012.07.30
  • Accepted : 2012.11.22
  • Published : 2012.11.30
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Abstract

Purpose: The purpose of this study is to examine in vivo osteogenesis of cultured human periosteal-derived cells and polydioxanone/pluronic F127 scaffold. Methods: Two one-year-old miniature pigs were used in this study. $2{\times}10^6$ periosteal-derived cells in 1 mL medium were seeded by dropping the cell suspension into the polydioxanone/pluronic F127 scaffold. These cell-scaffold constructs were cultured in osteogenic Dulbecco's modified Eagle's medium for 7 days. Under general anesthesia with azaperone and tiletamine-zolazepam, the mandibular body and ramus of the pigs were exposed. Three bony defects were created. Polydioxanone/pluronic F127 scaffold with periosteal-derived cells and the scaffold only were implanted into each defect. Another defect was left empty. Twelve weeks after implantation, the animals were sacrificed. Results: New bone formation was clearly observed in the polydioxanone/pluronic F127 scaffold with periosteal-derived cells. Newly generated bone was also observed in the scaffold without periosteal-derived osteoblasts and empty defect, but was mostly limited to the periphery. Conclusion: These results suggest that cultured human periosteal-derived cells have good osteogenic capacity in a polydioxanone/pluronic F127 scaffold, which provides a proper environment for the osteoblastic differentiation of these cells.

Keywords

References

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