Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Effect of Saururus chinensis BAILL Extract for Pharmacopuncture on the melanogenesis in B16F10 cells

삼백초 약침액이 B16F10 흑색종세포의 멜라닌 합성에 미치는 영향

  • Kim, Soo-Kyung (Dept. of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Kim, Dae-Sung (Dept. of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Woo, Won-Hong (Dept. of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Mun, Yeun-Ja (Dept. of Anatomy, College of Oriental medicine, Wonkwang University)
  • 김수경 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 김대성 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의과대학 해부학교실)
  • Received : 2012.02.29
  • Accepted : 2012.03.14
  • Published : 2012.03.27
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Abstract

Objectives : The purpose of this study was to investigate the melanogenesis inhibition effect of Saururus chinensis BAILL (SC) on in B16F10 melanoma cells. Methods : SC was fractionated ethanol extract by the hexane, ethyl acetate, butanol and water. We confirmed the inhibitory effect of tyrosinase activity and melanogenesis of all fraction samples. Results : Hexane fraction of Saururus chinensis BAILL (HSC), ethyl acetate of SC (ESC), and butanol of SC (BSC) were discovered to inhibit tysoinase activity and melanogenesis in the absence or presence of ${\alpha}$-MSH. However, water fraction of SC (WSC) did not affect tyrosinase activity and melanogenesis. In addition, all fractions did not inhibit the catalytic activity of cell-free tyrosinase from B16F10 melanoma cell lines. Conclusions : These results suggest that HSC, ESC and BSC reduce pigmentation by indirectly regulating tyrosinase.

Keywords

References

  1. Bell AA, Weeler MH. Biosynthesis and function of fungal melanin, Ann Rev Phtophathol. 1986 ; 24 : 411-51.
  2. Chen JS, Wei C, Marshall MR. Inhibition mechannism of Kojic acid on polyphenol oxidase. J Agric Food Chem. 1991 ; 39 : 1897-901.
  3. Urabe K, Aroca P, Tsukamoto K, Mascagna D, Paulumbo A, Prota G, Hearing VJ. The inherent cytotoxicty of melanin precursors. Biochim Biophys Acta. 1994 ; 1221 : 272-8.
  4. Slominski A, Tobin DJ, Shibahara S, Wortsman J. Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol Rev. 2004 ; 84(4) : 1155-228.
  5. Ferguson CA, Kidson SH. The regulation of tyrosinase gene transcription. Pigment Cell Res. 1997 ; 10(3) : 127-38.
  6. Hearing VJ, Tsukamoto K. Enzymatic control of pigmentation in mammals. FASEB J. 1991 ; 5(14) : 2902-9.
  7. Wong G, Pawelek J. Melanocyte-stimulating hormone promotes activation of pre-existing tyrosinase molecules in Cloudman S91 melanoma cells. Nature. 1975 ; 255 : 644-6.
  8. Hunt G, Todd C, Creswell JE, Thody AJ. Alpha-melanocyte stimulating hormone and its analogue Nle 4DPhe 7 alpha-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes. J Cell Sci. 1994 ; 107 : 205-11.
  9. 전국한의과대학 본초학교수. 본초학. 서울 : 영림사. 2004 : 371-2.
  10. 국가중의약관리국중화본초편찬위원회. 중화본초. 상해 : 상해과학기술출판사. 1999 : 419-20.
  11. 정보섭 외1인. 향약대사전. 서울 : 영림사. 1990 : 812-14.
  12. Koh MS. Antimicrobial activity of Saururus chinensis Baill Extract. J Korean Soc Food Sci Nutr. 2004 ; 33(7) : 1098-105.
  13. Cho HY, Cho CW, Song YS. Antioxidative and Anti-Inflammatory Effects of Saururus chinensis Methanol Extract in RAW 264.7 Macrophages. J Med Food. 2005 ; 8 : 190-7.
  14. Koh MS. Antimicrobial Activity of Saururus chinensis Baill Extract. J Korean Soc Food Sci Nutr. 2004 ; 33 : 1098-105.
  15. 박시경 외. 삼백초의 진통성분. 약학회지. 1998 ; 42 : 238-42.
  16. 서훈석, 정봉환, 조용구. 짚신나물, 삼백초의 항산화와 항암활성 효과. 한국약용작물학회지. 2008 ; 16 : 139-43.
  17. 박대중, 이장천. 삼백초 에탄올추출물의 항산화능 및 피부 미백작용 연구. 대한본초학회지. 2008 ; 23 : 193-202.
  18. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 ; 65(1-2) : 55-63.
  19. Martinez-Esparza M, Jimenez-Cervantes C, Solano F, Lozano JA, Garcia-Borron JC. Mechanisms of melanogenesis inhibition by tumor necrosis factor-alpha in B16/F10 mouse melanoma cells. Eur J Biochem. 1998 ; 255(1) : 139-46.
  20. Hosoi J, Abe E, Suda T, Kuroki T. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid.. Cancer Res. 1985 ; 45(4) : 14748.
  21. Nerya O, Vaya J, Musa R, Izrael S, Ben-Arie R, Tamir S. Glabrene and isoliquiritigenin as tyrosinase inhibitors from licorice roots. J Agric Food Chem. 2003 ; 51(5) : 1201-7.
  22. Department of Food Science, Agricultural Research Organization, Bet Dagan, Israel. Effect of kojic acid on the oxidation of L-DOPA, norepinephrine, and dopamine by mushroom tyrosinase. Pigment Cell Res. 1995 ; 8(5) : 234-40.
  23. Chakraborty AK, Funasaka Y, Komoto M, Ichihashi M. Effect of arbutin on melanogenic proteins in human melanocytes. Pigment Cell Res. 1998 ; 11(4) : 206-12.
  24. Shin NH, Ryu SY, Choi EJ, Kang SH, Chang IM, Min KR, Kim Y. Oxyresveratrol as the potent inhibitor on dopa oxidase activity of mushroom tyrosinase. Biochem Biophys Res Commun. 1998 ; 243(3) : 801-3.
  25. 이승연, 김진만, 오현철, 임숙정, 황충연, 문연자, 우원홍. 더덕추출물이 멜라닌생성에 미치는 영향. 대한한의학 방제학회지. 2002 ; 10(2) : 199-211.
  26. 문연자, 이성원, 임숙정, 송채석, 이관순, 임규상, 우원홍. 천화문 수추출물이 B16 흑색종 세포의 멜라닌 생성 억제 효과. 한국전통의학지. 2000 ; 10(2) : 149-59.
  27. 천현자, 최은영, 윤성찬, 남항우, 백승화, 우원홍. 백출의 에탄올 추출물에 의한 melanin 생성 억제 효과. 약학회지. 2001 ; 45(3) : 269-75.
  28. 임난영, 권강주, 김윤석, 백순기, 임주락, 문연자, 우원홍. 사삼메탄올 추출물의 멜라닌 생성 억제 효과. 동의생리병리학회지. 2004 ; 18(3) : 747-53.
  29. 신기돈, 김대성, 이장천, 문연자, 우원홍, 이영철. 인진 에탄올추출물이 ${\alpha}$-MSH로 유도된 과색소 형성에 미치는 영향. 동의생리병리학회지. 2009 ; 23(3) : 574-80.
  30. 노성택, 김대성, 이성진, 박대중, 이장천, 임규상, 우원홍, 문연자. 교맥 에탄올 추출물의 피부 미백기전 연구. 동의생리병리학회지. 2007 ; 21(5) : 1243-9.
  31. Yun JY, Roh E, Son JK, Lee SH, Seo CS, Hwang BY, Han SB, Kim Y. Effect of saucerneol D on melanin production in cAMPelevated melanocytes. Arch Pharm Res. 2011 ; 34(8) : 1339-45.
  32. Seo CS, Lee WH, Chung HW, Chang EJ, Lee SH, Jahng Y, Hwang BY, Son JK, Han SB, Kim Y. Manassantin A and B from Saururus chinensis inhibiting cellular melanin production. Phytother Res. 2009 ; 23(11) : 1531-6.
  33. Lee HD, Lee WH, Roh E, Seo CS, Son JK, Lee SH, Hwang BY, Jung SH, Han SB, Kim Y. Manassantin A inhibits cAMP-induced melanin production by down-regulating the gene expressions of MITF and tyrosinase in melanocytes. Exp Dermatol. 2011 ; 20(9) : 761-3.
  34. Chen QX, Kubo I. Kinetics of mushroom tyrosinase inhibition by quercetin. J Agric Food Chem. 2002 ; 50(14) : 4108-12.
  35. Nitoda T, Isobe T, Kubo I. Effects of phenolic compounds isolated from Rabdosia japonica on B16-F10 melanoma cells. Phytother Res. 2008 ; 22(7) : 867-72.
  36. Drewa G, Schachtschabel DO, Paigan K, Grzanka A, Sujkowska R. The influence of rutin on the weight, metastasis and melanin content of B16 melanotic melanoma in C57BL/6 mice. Neoplasma. 1998 ; 45(4) : 266-71.