Journal of the Korean Crystal Growth and Crystal Technology (한국결정성장학회지)

The Korea Association of Crystal Growth (한국결정성장학회)
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Bioactivity behavior of biphasic calcium phosphate powders prepared by co-precipitation method

공침법으로 합성된 biphasic calcium phosphate 분말의 생체활성 거동

  • Kim, Tae-Wan (School of Materials Science Engineering, Pusan National University) ;
  • Kim, Dong-Hyun (School of Materials Science Engineering, Pusan National University) ;
  • Jin, Hyeong-Ho (School of Materials Science Engineering, Pusan National University) ;
  • Lee, Heon-Soo (Division of Electrical and Electronic Engineering, Daegu Technical University) ;
  • Park, Hong-Chae (School of Materials Science Engineering, Pusan National University) ;
  • Yoon, Seog-Young (School of Materials Science Engineering, Pusan National University)
  • Received : 2012.02.27
  • Accepted : 2012.03.23
  • Published : 2012.04.30
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Abstract

The co-precipitation technique has been applied to synthesize biphasic calcium phosphate (BCP). $Ca(NO_3)_2{\cdot}4H_2O$ and $(NH_4)_2HPO_4$ as the starting materials was used. X-ray diffraction (XRD) and Fourier transformed infrared (FT-IR) spectroscopy were used to characterize the structure of as-synthesized and calcined BCP powders. After immersion in Hanks' Balanced Salt Solution (HBSS), for 1 week a precipitation started to be formed with individual small granules on the specimen surface. An MTT assay indicated that BCP powders have no cytotoxic effects on MG-63 cells, and that they have good biocompatibility.

BCP(biphasic calcium phosphate) 분말을 제조하기 위하여 $Ca(NO_3)_2{\cdot}4H_2O$, $(NH_4)_2HPO_4$를 출발 물질로 공침법(co-precipitation process)을 이용하여 합성하였다. 합성된 분말의 열처리 전 후의 HAp(Hydroxyapatite)/${\beta}$-TCP(${\beta}$-Tricalcium phosphate) 결정상 비율 및 분광학적 특성을 XRD 및 FT-IR를 이용하여 분석하였다. BCP 분말의 생체활성 거동을 평가하기 위하여 Hanks' Balanced Salt Solution(HBSS)에 침적시켜 시간에 따라 형상, 이온농도의 변화 및 결정상을 분석한 결과 낮은 결정화도의 HAp가 생성됨을 확인하였다. BCP분말의 세포독성 평가에서도 대조군에 비하여 세포성장률이 우수함을 관찰하였다.

Keywords

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