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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Effect of interferon-γ on the fusion of mononuclear osteoclasts into bone-resorbing osteoclasts

  • Kim, Jeung-Woo (Department of Orthopaedic Surgery, Division Rheumatology, Wonkwang University School of Medicine) ;
  • Lee, Myeung-Su (Department of Internal Medicine, Division Rheumatology, Wonkwang University School of Medicine) ;
  • Lee, Chang-Hoon (Department of Internal Medicine, Division Rheumatology, Wonkwang University School of Medicine) ;
  • Kim, Ha-Young (Department of Internal Medicine, Division of Endocrinology and Metabolism, Sanbon Medical Center) ;
  • Chae, Soo-Uk (Department of Orthopaedic Surgery, Gunsan Medical Center) ;
  • Kwak, Han-Bok (Department of Anatomy, Wonkwang University School of Medicine) ;
  • Oh, Jae-Min (Department of Anatomy, Wonkwang University School of Medicine)
  • Received : 2011.12.13
  • Accepted : 2012.01.16
  • Published : 2012.05.31
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Abstract

Osteoclasts are multinucleated cells that are formed by the fusion of pre-fusion osteoclasts (pOCs). The fusion of pOCs is known to be important for osteoclastic bone resorption. Here, we examined the effect of IFN-${\gamma}$ on the fusion of pOCs. IFN-${\gamma}$ greatly increased the fusion of pOCs in a dose-dependent manner. Furthermore, IFN-${\gamma}$ induced pOC fusion even in hydroxyapatite-coated plates used as a substitute for bone. The resorption area of pOCs stimulated with IFN-${\gamma}$ was significantly higher than that of the control cells. IFN-${\gamma}$ induced the expression of dendritic cell-specific transmembrane protein (DC-STAMP), which is responsible for the fusion of pOCs. IFN-${\gamma}$ enhanced DC-STAMP expression in a dose-dependent manner. The mRNA expression of c-Fos and nuclear factor of activated T cells (NFAT) c1 was enhanced in the pOCs treated with IFN-${\gamma}$. Taken together, these results provide a new insight into the novel role of IFN-${\gamma}$ on the fusion of pOCs.

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