YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effect of Ochnaflavone as An Immunoadjuvant

Ochnaflavone의 면역보조제 효과

  • Park, Minjoo (ImmunoMicrobiology Lab, College of Pharmacy, Dongduk Women's University) ;
  • Rhew, Ki Yon (ImmunoMicrobiology Lab, College of Pharmacy, Dongduk Women's University) ;
  • Han, Yongmoon (ImmunoMicrobiology Lab, College of Pharmacy, Dongduk Women's University)
  • 박민주 (동덕여자대학교 약학대학 면역미생물학 실험실) ;
  • 유기연 (동덕여자대학교 약학대학 면역미생물학 실험실) ;
  • 한용문 (동덕여자대학교 약학대학 면역미생물학 실험실)
  • Received : 2012.09.21
  • Accepted : 2012.09.30
  • Published : 2012.12.31
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Abstract

In this present study, we determined whether or not there is an immunoadjuvant effect of ochnaflavone, a biflavone isolated from Lonicera japonica. As an antigenic source, the cell wall (CACW) of Candida albicans, a fungal pathogen, was used. CACW consists of 95% carbohydrate (mannan). In the experiments, BALB/c mice were immunized with emersion forms of CACW combined with or without ochnaflavone (Och) in the presence of IFA containing mineral oil or CACW alone. Then, the amounts of antisera collected from these mice groups were measured by the ELISA method. Data from these experiments showed that CACW combined with Och (CACW/Och/IFA) provoked the production of antisera app. 2.2 or 5 times more than the corresponding CACW/IFA or CACW alone (CACW/DPBS), respectively, in mice (P<0.05). We further examined the immune response type induced by Och. Analysis of the values of the IgG1/IgG2a ratios obtained from IgG isotyping revealed that Och induced Th2-immunity more dominantly than Th1. This finding was confirmed by cytokine profile. CACW/Och/IFA formulation induced IL-4 (Th2-type cytokine) more than IFN${\gamma}$ (Th1-type cytokine) as compared with CACW/IFA and CACW/DPBS formulations (P<0.05). All data combined, Och appears to have an immunoadjuvant activity that may convert Th1 immunity into Th2 immunity.

Keywords

References

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