Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Optimal Extraction Conditions of Flavonoids from Onion Peels via Response Surface Methodology

양파껍질로부터 Flavonoid 물질의 추출조건 최적화

  • Jeon, Seon-Young (Dept. of Food and Nutrition, Changwon National University) ;
  • Baek, Jeong-Hwa (Dept. of Food and Nutrition, Changwon National University) ;
  • Jeong, Eun-Jeong (Dept. of Food and Nutrition, Changwon National University) ;
  • Cha, Yong-Jun (Dept. of Food and Nutrition, Changwon National University)
  • 전선영 (창원대학교 식품영양학과) ;
  • 백정화 (창원대학교 식품영양학과) ;
  • 정은정 (창원대학교 식품영양학과) ;
  • 차용준 (창원대학교 식품영양학과)
  • Received : 2012.02.07
  • Accepted : 2012.04.03
  • Published : 2012.05.31
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Abstract

The objective of this study was to set the optimal extraction condition of flavonoids from onion peels as a by-product generated from the onion industry without suitable processing. Four independent variables, affecting extraction conditions, which are solvent concentration ($X_1$), extraction temperature ($X_2$), pH of the solvent ($X_3$), and solvent ratio to onion peel ($X_4$) were optimized using response surface methodology (RSM). A model equation obtained from RSM is 0.772 of R-square and 0.278 of lack of fit (p>0.05) for the optimal extraction conditions. From the ridge analysis, the conditions flavoring the highest extraction were solvent concentration (v/v) of 70%, extraction temperature of $40^{\circ}C$, extraction solvent pH of 5.3, and a solvent ratio to onion peel ratio of 1:63 (w/v). The flavonoid content obtained under optimal conditions showed 302.63 mg/g, which is 1.12 times higher than the prediction value.

본 연구에서는 양파가공부산물인 양파껍질로부터 반응표면분석법을 이용하여 유효성분인 flavonoid 물질의 최적 추출조건을 설정하고자 하였다. 양파껍질의 일반성분은 수분 4.68%, 회분 6.67%, 조단백질 및 조지방이 5.03%, 0.71%이었고, 무기질은 Ca이 20.07 g/kg으로 가장 많은 함량을 차지하였고 K이 5.54 g/kg, Mg 2.17 g/kg의 순으로 함량이 많았다. 중심합성계획법에 의한 flavonoid 물질의 최적 추출조건은 다중회귀분석 및 능선분석 결과, 결정계수는 0.772이었으며, 적합결여검증(p>0.05)에서는 0.278, 전체적인 모형에서 유의성(p<0.05)을 보여 반응모형이 적합한 것으로 판단되었다. 따라서 최대점을 만족하는 각 변수의 조건은 추출용매의 농도는 70%, 추출온도 $40^{\circ}C$, 추출용매의 pH 5.3, 원료에 대한 추출용매의 비율은 1:63(w/v)이었다.

Keywords

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