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Biological Properties of Vero Cell-Adapted Newcastle Disease Virus

Vero 세포적응 뉴캣슬병 바이러스의 생물학적 특성

  • Choi, Kang-Seuk (Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Park, Mi-Ja (Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kye, Soo-Jeong (Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kim, Ji-Ye (Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kwon, Jun-Hun (Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)
  • 최강석 (농림수산검역검사본부 조류질병과) ;
  • 박미자 (농림수산검역검사본부 조류질병과) ;
  • 계수정 (농림수산검역검사본부 조류질병과) ;
  • 김지예 (농림수산검역검사본부 조류질병과) ;
  • 권준헌 (농림수산검역검사본부 조류질병과)
  • Received : 2012.03.10
  • Accepted : 2012.05.16
  • Published : 2012.06.30

Abstract

Newcastle disease virus (NDV) Kr005/V strain was generated through 55 serial passages of NDV Kr005 strain in Vero cells. The Kr005/V virus yielded high infective titers of $10^{7.8}$ $TCID_{50}/mL$ in Vero cells and the infected cells showed cytopathic effects such as marked cell rounding, though less frequent syncytia. The Kr005/V virus was heat-stable and classified into the lentogenic type with a Mean Death Time (MDT) of 120h or greater while the Kr005 strain was heat-labile and velogenic (MDT of 49.6 h). Only the single amino acid substitution (T to S) was observed at position 433 of the HN protein of the Kr005/V strain, whereas no amino acid change was found in the F protein. The Kr005/V input virus correlated well (correlation coefficient $r^2$=0.97) with the Kr005 virus when ten field sera were tested by virus neutralization test. The biological properties and usefulness of Vero cell-adapted Kr005/V virus were discussed.

본 연구를 통하여 Vero 세포에서 강독형 NDV인 Kr005주를 55대 연속 계대하여 Vero 세포에 적응된 Kr005/V 주를 작성하였다. Kr005/V 주는 Vero 세포에서 $10^{7.8}/mL$의 높은 바이러스 증식성을 보였으며, 감염세포는 cell rounding과 같은 CPE를 형성하였다. 또한 이열성(heat labile) NDV Kr005주와 달리 NDV Kr005/V 주는 내열성(thermostable)을 보였다. 계태아에서 평균 치사 시간(MDT)를 측정한 결과, 강독형인 Kr005주(49.6시간의 MDT)와 달리 Kr005/V 주는 120시간이상의 MDT를 보여 약독형 바이러스(lentogenic virus)로 분류되었다. Kr005/V 주의 HN 단백질과 F 단백질의 염기서열을 분석한 결과, HN 단백질의 443번째 아미노산에서만 변이(T에서 S)가 관찰되었다. Kr005/V 주와 Vero 세포를 사용하여 야외 닭 혈청을 대상으로 혈청중화 항체가를 측정한 결과, Kr005주와 CEF 세포를 사용하여 측정한 혈청중화 항체가와 97%의 상관성을 나타내었다.

Keywords

References

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