Journal of the Korean Crystal Growth and Crystal Technology (한국결정성장학회지)

The Korea Association of Crystal Growth (한국결정성장학회)
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Biocompatibility of porous hydroxyapatite ceramics prepared from bovine bones

소 뼈로부터 제조한 다공형 하이드록시아파타이트 세라믹스의 생체친화 특성

  • Lee, Jong-Kook (Department of Advanced Materials Engineering, Chosun University, BK21 Education Center of Mould Technology for Advanced Materials & Parts, Chosun University) ;
  • Ko, Young-Hwa (Department of Advanced Materials Engineering, Chosun University, BK21 Education Center of Mould Technology for Advanced Materials & Parts, Chosun University) ;
  • Lee, Nan-Hee (Department of Advanced Materials Engineering, Chosun University, BK21 Education Center of Mould Technology for Advanced Materials & Parts, Chosun University)
  • 이종국 (조선대학교 신소재공학과, BK21 첨단소재부품 금형기술 인력양성사업단) ;
  • 고영화 (조선대학교 신소재공학과, BK21 첨단소재부품 금형기술 인력양성사업단) ;
  • 이난희 (조선대학교 신소재공학과, BK21 첨단소재부품 금형기술 인력양성사업단)
  • Received : 2012.04.19
  • Accepted : 2012.05.18
  • Published : 2012.06.30
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Abstract

Natural hydroxyapatite powder was obtained from the calcination of bovine bones and its porous compacts were fabricated by pressureless sintering at 1100 and $1200^{\circ}C$ for 1h. To evaluate and compare their biocompatibility with porosity, we investigated the support of osteoblast cells growth and cytotoxicity using the MG-63 cell line model in vitro. Sintered hydroxyapatite ceramics have a porous microstructure with a relative density of 65 % at $1100^{\circ}C$ and 82 % at $1200^{\circ}C$. Cells adherence to the surface of hydroxyapatite ceramics was observed in a day after the cell culture, and the spreading of cytoplasm around the nucleus was shown after 3 day cell culture. Most of cells were extended to the surface of hydroxyapatite through the wide area. Cell viability was nearly the same till 3 days culturing. But the rate of cell growth is higher in the specimen sintered at $1100^{\circ}C$ than that of $1200^{\circ}C$. It indicates that the porosity is an important factor to enhance the cell viability in the porous hydroxyapatite ceramics derived from bovine bones.

소뼈를 하소하여 천연 하이드록시아파타이트 분말을 얻은 다음, $1100^{\circ}C$ 및 $1200^{\circ}C$로 상압 소결하여 기공율이 각각 35 % 및 18 %인 다공성 하이드록시아파타이트를 제조한 후 이들 시편의 생체친화성을 평가하였다. 골아세포의 성장속도과 세포독성의 유무에 따라 평가되는 생체친화성은 MG-63 세포의 부착능과 분화되어 증식되는 세포의 수로 측정하였다. in vitro에서 세포 반응을 관찰한 결과 소뼈로부터 제조된 하이드록시아파타이트 시편은 독성이 없고 생체 친화성이 좋은 시편이었다. 세포배양 1일 후부터 하이드록시아파타이트 표면에 세포가 부착되었고, 세포배양 후 3일 후에는 핵 주위로 세포질이 퍼져나갔으며, 5일 후에는 부착된 세포의 수가 크게 증가하였다. 배양 초기에는 밀도에 관계 없이 세포의 부착능이 유사하였지만, 세포 배양 시간이 증가하면서 기공율이 높은 시편에서 세포의 부착 및 성장이 더 활발하게 진행되었다.

Keywords

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