Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Physicochemical Characteristics and Antioxidant Activities of Luffa cylindrica (L.) Roem

수세미오이의 이화학적 특성과 항산화활성

  • Received : 2012.02.20
  • Accepted : 2012.05.22
  • Published : 2012.06.30
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Abstract

Physiochemical characteristics of Luffa cylindrica (L.) Roem and antioxidant activities of its four extracts were analyzed. The chemical composition of L. Roem contained 93.69% moisture, 1.64% crude fiber, 0.52% crude protein, 0.27% crude fat, and 0.06% crude ash. The major minerals were potassium, phosphrous, and calcium. We also analyzed the major organic acids, acetic acid and succinic acid. In free amino acids, ${\beta}$-aminoisobutyric acid showed the highest concentration (100.74 mg/100 g), followed by phosphoethanolamine, urea, asparagine, and valine. Glutamic acid was the highest hydrolyzed amino acid with 1,039.99 mg/100 g followed by aspartic acid, lysine, and arginine. Four extracts from L. Roam, hot-water (LCH), cold-water (LCC), 80% ethanol (LCE), and methanol (LCM), were prepared. Total phenolic and flavonoid levels of LCE exhibited higher than three extracts. The antioxidant potential of extracts from L. Roem were investigated using DPPH, ABTS, and NBT assays. Of the four extracts, LCE had relatively high antioxidant capability on DPPH and superoxide anion radical scavenging activities (12.13%, 16.88%, and 26.61%). Based on the above results, it is suggested that an 80% ethanolic extract from L. Roem was a natural antioxidant material for health food and should be a good ingredient for functional food.

본 연구는 수세미오이의 일반성분과 생리활성 평가를 위한 추출방법에 따른 항산화활성을 분석하였다. 수세미오이는 수분 93.69%, 조섬유 1.64%, 조단백 0.52%, 조지방 0.27%, 조회분 0.06%을 함유하고 있었다. 주요 무기질로 K, P, Ca, Mg 등이 분석되었고, 주요 유기산은 아세트산과 호박산으로 나타났다. 유리아미노산으로 ${\beta}$-aminoisobutyric acid가 100.74 mg/100 g으로 가장 높게 나타났고 phosphoethanolamine, urea, asparagine, valine의 순으로 분석되었다. 구성 아미노산으로 glutamic acid가 1,039.99 mg/100 g으로 가장 높았으며 aspartic acid, lysine, arginine 순으로 분석되었다. 수세미오이의 생리활성을 평가하기 위해 물, 80% 에탄올, 메탄올을 이용하여 열수추출물(LCH), 냉수추출물(LCC), 80% 에탄올추출물(LCE), 메탄올추출물(LCM)을 수득하고 각 추출물간 라디칼 소거활성에 기인한 항산화활성을 비교하였다. 총 페놀성 화합물 및 플라보노이드 함량 측정에서는 LCE가 다른 추출물에 비하여 통계적으로 유의한 차이를 나타내었다. 또한, DPPH radical 소거활성과 ABTS 라디칼소거활성 및 superoxide anion radical 소거활성에서도 80% 에탄올추출물이 각각 12.13%, 16.88%, 26.61%로 다른 추출물에 비해 높은 활성을 나타내었다. 이의 결과를 바탕으로 수세미오이 80% 에탄올추출물은 항산화활성을 갖는 기능성식품 소재로써 활용될 수 있을 것이며 수세미오이의 성분함량은 수세미오이를 이용한 식품개발의 기초자료로 활용할 수 있을 것으로 판단된다.

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