Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Cultivated Orostachys japonicus Induces Apoptosis in Human Colon Cancer Cells

인체 대장암 세포주 SW480에서 재배 와송의 세포 사멸 유도 효과

  • Kim, Jae-Yong (Department of Food and Nutrition, Sunchon National University) ;
  • Jung, Eun-Jung (Department of Food and Nutrition, Sunchon National University) ;
  • Won, Yeong-Seon (Department of Food and Nutrition, Sunchon National University) ;
  • Lee, Ju-Hye (Department of Food and Nutrition, Sunchon National University) ;
  • Shin, Dong-Young (Department of Horticultural Science, Sunchon National University) ;
  • Seo, Kwon-Il (Department of Food and Nutrition, Sunchon National University)
  • 김재용 (순천대학교 식품영양학과) ;
  • 정은정 (순천대학교 식품영양학과) ;
  • 원영선 (순천대학교 식품영양학과) ;
  • 이주혜 (순천대학교 식품영양학과) ;
  • 신동영 (순천대학교 원예학과) ;
  • 서권일 (순천대학교 식품영양학과)
  • Received : 2011.11.29
  • Accepted : 2012.03.31
  • Published : 2012.06.30
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Abstract

This study was performed to elucidate the anticancer activities and the mechanism of chloroform fractions from cultivated Orostachys japonicus (CFCOJ) in human colon cancer cells. CFCOJ markedly decreased viable cell numbers in both a dose-dependent and time-dependent manner within SW480 cells. Cell death induced by CFCOJ increased cell populations in the sub-G1 phase, as well as the formation of apoptotic bodies, nuclear condensation, and induced DNA fragmentation. CFCOJ-induced apoptosis was associated with the activation of initiator caspase-8 and -9, as well as the effector caspase-3. CFCOJ also stimulated Bid cleavage, indicating that the apoptotic action of caspase-8-mediated Bid cleavage leads to the activation of caspase-9. CFCOJ increased the expression of the proapoptotic protein, Bax, and decreased the expression of the antiapoptotic protein, Bcl-2. These results indicate that CFCOJ exert anticancer effects on human colon cancer SW480 cells through a caspase-dependent apoptotic pathway.

재배 와송의 항암효과를 입증하기 위해 SW480 대장암세포에서 재배 와송 추출물의 apoptosis 유도 효과 및 그 기전에 미치는 영향에 대해 알아보았다. 재배 와송과 천연 와송 메탄올 추출물의 암세포 성장 억제능을 측정한 결과, 재배 와송은 천연 와송과 유사하게 농도 의존적으로 높은 암세포 증식억제효과를 보였으며 특히 농도 300 ${\mu}g$/mL의 재배 와송 메탄올 추출물에서는 천연 와송 메탄올 추출물 보다 더 높은 억제 효과를 나타내었다. 또한 재배 와송 메탄올 추출물을 분획하여 얻은 각각의 물, 헥산, 클로로포름, 에틸아세테이트 및 부탄올 분획물 중 와송 클로로포름 분획물에서 암세포의 증식 억제효과가 가장 높게 나타났다. 이러한 와송 클로로포름 분획물의 apoptosis 유도 효과는 Sub-G1기의 함량 증가와 핵의 응축 및 apoptotic bodies 생성 그리고 DNA 분절을 통해 나타났다. 한편, 와송 클로로포름 분획물의 caspase 활성은 caspase inhibitor 처리군에서 암세포의 증식 억제효과가 확연히 저해됨을 나타내었으며 각각의 caspase 활성 중 caspase-3 및 -9의 활성을 증가시켰다. 또한 와송 클로로포름 분획물은 apoptosis 관련 단백질들인 Bid, Bcl-2 및 PARP의 발현을 농도 의존적으로 감소시켰으며 tBid 및 Bax 단백질의 발현을 현저하게 증가시켰다. 본 연구 결과, 재배 와송과 천연 와송은 유사한 암세포 성장 억제 효과를 보였으며 재배 와송의 클로로포름 추출물은 caspase 의존적인 경로를 통해 apoptosis를 일으켜 세포 사멸 효과를 나타내는 것을 확인할 수 있었다.

Keywords

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