Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
권호 표지
DOI QR코드

DOI QR Code

Comparative Study of Pathogenicity Following Single or Coinfection with Edwardsiella tarda and Streptococcus iniae in Olive Flounder, Paralichthys olivaceus

넙치, Paralichthys olivaceus에서 Edwardsiella tarda와 Streptococcus iniae 단독 또는 혼합감염의 병원성 비교 연구

  • Received : 2012.07.20
  • Accepted : 2012.08.08
  • Published : 2012.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Olive flounders, Paralichthys olivaceus, were intraperitoneally challenged with Edwardsiella tarda or Streptococcus iniae or both bacteria simultaneously. The pathogenicity was respectively compared with blood chemical using alanine aminotransferase (ALT), aspatate aminotransferase (AST), glucose and total protein, lysozyme activity, bacterial number of kidney and spleen, histopathological change, and cumulative mortality. The tested group of coinfection showed increased cumulative mortality, bacterial number of kidney and spleen, AST and histopathological change, but not in lysozyme activity compared with others. This study provides support for the conclusion that simultaneous infection with Edwardsiella tarda and Streptococcus iniae in a susceptible host results in higher pathogenicity, leading to the increment of bacterial number and the destruction of the internal organs.

양식 넙치에 Edwardsiella tarda 또는 Streptococcus iniae를 단독 또는 혼합감염하여 넙치에서 병원성을 비교하였다. 넙치에 단독 또는 혼합감염 후 혈액생화학적 성상으로 alanine aminotransferase (ALT), aspatate aminotransferase (AST), glucose 농도, total protein 농도를 조사하였으며, 라이소자임 활성, 신장과 비장 조직 중의 세균수, 조직병리학적 변화와 누적폐사율을 조사하였다. 혼합감염구는 다른 시험구에 비하여 누적폐사율, 신장과 비장 조직 중의 세균수, AST활성과 병리조직학적 변화가 크게 나타났으나, 라이소자임 활성에서는 유의적인 차이가 보이지 않았다. 따라서 E. tarda와 S. iniae의 혼합감염은 신장과 비장 조직 중의 세균수 증가와 내부 장기의 손상으로 숙주에 높은 병원성을 나타낸 것으로 판단된다.

Keywords

References

  1. 김강웅.권문경.김경덕.김신권.박명애.손맹현(2010). 배합사료가 양식장 넙치의 성장 및 건강도 향상에 미치는 영향, 수산해양교육연구 22, 529-536.
  2. 김은희(1999). 넙치 (Paralichthys olivaceus)에서 분리된 Edwardsiella tarda의 약제내성 전달성 R plasmid, 한국어병학회지 12, 115-121.
  3. 김진우.정승희.박명애.도정완.최동림.지보영.조미영.김명석.최혜승.김이청.이주석.이창훈.방종득.박미선.서정수(2006). 2000년-2006년 하절기 양식어류의 병원체 감염현황. 한국어병학회지 19, 207-214.
  4. 김진우.조미영.박경현.원경미.최혜승.김명석.박명애(2010). 2005년부터 2007년 사이 양식 넙치, Paralichthys olivaceus 질병에 대한 통계 자료, 한국어병학회지 23, 369-377.
  5. 김현정.우승호.김진우.박수일(2005). Streptococcus iniae의 형태학적 특성과 병원성, 한국어병학회지, 18(2), 167-178.
  6. 정용욱.강철영.김민주.허문수.오덕칠.강봉조(2006). 제주지역 양식넙치의 연쇄구균증 발생동향 및 원인균에 대한 분자적 동정, 한국미생물학회지 42, 199-204.
  7. 조미영.김명석.권문경.지보영.최혜승.최동림.박경현.이창훈.김진도.이주석.오윤경.이덕찬.박신후.박명애(2007). 2005년부터 2006년 사이 우리나라 양식 넙치, Paralichthys olivaceus의 세균성 질병에 대한 역학조사, 한국어병학회지 20, 51-60.
  8. 조미영.김명석.최혜승.박경현.김진우.박미선.박명애(2008). 양식 넙치(Paralichthys olivaceus) 질병에 대한 통계적 관찰, 한국어병학회지 21, 271-278.
  9. 하수진.우승호.이덕찬.박수일(2008). 넙치, Paralichthys olivaceus의 비특이적 방어기작에 대한 Edwardsiella tarda균의 반응, 한국어병학회지 21, 189-199.
  10. Ainsworth, A.J., and Chen, D.X.(1990). Differences in the phagocytosis of four bacteria by channel catfish neutrophils, Dev. Comp, Immunol., 14, 201-209. https://doi.org/10.1016/0145-305X(90)90091-R
  11. Barham, W.T., Smit, G.L. and Schoonbee, H.J.(1980). The hematological assessment of bacterial infection in rainbow trout Salmo gairdneri, J. Fish Biol., 17, 275-281. https://doi.org/10.1111/j.1095-8649.1980.tb02761.x
  12. Canfield, P.J., Church, D.B. and Gallagher, C.H(1985). Veterinary clinical enzymology: Labortatory aids for diagnosis and evaluation of disease, University of Sydney, Sydney, Australia.
  13. Chen, C.-Y., Gregory, A.W. and Paul, R.B.(2004). Comparative blood chemistry and histopathology of tilapia infected with Vibrio vulunificus of Streptococcus iniae or exposed to carbon tetrachloride, gentamicin, or copper sulfate, Aquaculture, 239, 421-443. https://doi.org/10.1016/j.aquaculture.2004.05.033
  14. Das, A., Sahoo, P.K., Mohanty, B.R. and Jena, J.K.(2011). Pathophysiology of experimental Aeromonas hydrophila infection in Puntius sarana: Early changes in blood and aspects of the innate immune-related gene expression in survivors, 142, 207-218. https://doi.org/10.1016/j.vetimm.2011.05.017
  15. Duncan, D.B.(1955). Multiple-range and multiple F tests, Biometrics, 11, 1-42. https://doi.org/10.2307/3001478
  16. Grizzle, J.M. and Kiryu, Y.(1993). Histopathology of gill, liver and pancreas and serum enzyme levels of channel catfish infected with Aeromonas hydrophila complex, J. Aquat. Anim. Health., 5, 36-50. https://doi.org/10.1577/1548-8667(1993)005<0036:HOGLAP>2.3.CO;2
  17. Hikima, J., Minagawa, S., Hirono, I. and Aoki, T.(2001). Molecular cloning, expression and evolution of the japanese flounder goose-type lysozyme gene, and the lytic activity of its recombinant protein, Biochemical et Biophysica Acta, 1520, 35-44. https://doi.org/10.1016/S0167-4781(01)00248-2
  18. Hirono, L., Tange, N. and Aoki, T.(1997). Iron regulated hemolysin gene from Edwardsiella tarda, Mol. Microbiol., 2, 851-856.
  19. Kusuda, T. and Kawai, K.(1998). Bacterial diseases of cultured marine fish in Japan, Fish Pathol., 33, 221-227. https://doi.org/10.3147/jsfp.33.221
  20. Moyner, K., Ored, K.H., Sevatdal, S. and Heum, M.(1993). Changes in nonspecific immune parameters in atlantic salmon, Salmo salar L., induced by Aeromonas salmonicida infection, Fish and Shellfish Immunol., 3, 253-256. https://doi.org/10.1006/fsim.1993.1025
  21. Oh, M.-J., Kim, W.-S., Kitamura, S.-I., Lee, H.-K., Son, B.W., Jung, T.-S. and Jung, S.-J.(2006). Change of pathogenicity in olive flounder Paralichthys olivaceus by co-infection of Vibrio harveyi, Edwardsiella tarda and marine birnavirus, Aquaculture, 257, 156-160. https://doi.org/10.1016/j.aquaculture.2006.03.009
  22. Parry, R.M., Chandau, R.C. and Shahani, R.M.(1965). A rapid and sensitive assay of muramidase, Proc. Soc. Exp. Biol. Med., 119, 384-386. https://doi.org/10.3181/00379727-119-30188
  23. Saeed, M.O.(1995). Association of Vibrio harveyi with mortalities in cultured marine fish in Kuwait, Aquaculture, 136, 21-29. https://doi.org/10.1016/0044-8486(95)01045-9
  24. Saurabh, S. and Sahoo, P.K.(2007). Ich (white spot) disease in carps, Aqua International, 14, 27-19.
  25. Siwicki, A. and Studnicka, M.(1987). The phagocytic ability of neutrophils and lysozyme activity in experimentally infected carp, Cyprinus carpio L. Journal of Fish Biology, 31A, 57-60.
  26. Spanggaard, B., Huber, I., Nielsen, J., Nielsen, T. and Gram, L.(2000). Proliferation and location of Vibrio anguillarum during infection of rainbow trout, Oncorhynchus mykiss. J. Fish Dis., 23, 423-427. https://doi.org/10.1046/j.1365-2761.2000.00257.x
  27. SPSS Inc.(1997). SPSS Base 7.5 for Window, SPSS Inc., 444N. Michigan Avenue, Chicago, IL, 60611.
  28. Ullah, M.A. and Arni, T.(1983). Pathological activities of the naturally occurring strains of Edwardsiella tarda, Fish Pathol., 3, 65-70.
  29. Wedemeyer, G. and McLeay, D.J.(1981). Methods of determine the tolerance of fishes to environmental stressors, In Stress in Fish, 247-275, Pickering, A.D., Academic Press, London.
  30. Wedemeyer, G.A., Barton, B.A. and McLeay, D.J.(1990). stress and acclimation, In Methods for Fish Biology, 451-489.

Cited by

  1. Effects of Dietary Inclusion of Various Additives on Growth Performance and Immune Responses in Juvenile Olive Flounder(Paralichthys olivaceus) vol.25, pp.6, 2013, https://doi.org/10.13000/JFMSE.2013.25.6.1285