Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Ishige sinicola Extracts Induce Apoptosis via Activation of a Caspase Cascade in Human HeLa Cells

넓패 추출물이 HeLa 자궁암세포의 세포사멸에 미치는 영향

  • Cho, Byoung-Ok (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Ryu, Hyung-Won (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • So, Yang-Kang (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jin, Chang-Hyun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Myung-Woo (Dept. of Culinary Nutrition, Woosong University) ;
  • Kim, Wang-Geun (Rare Isotope Science Project Team, Institute for Basic Science) ;
  • Jeong, Il-Yun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 조병옥 (한국원자력연구원 첨단방사선연구소) ;
  • 류형원 (한국원자력연구원 첨단방사선연구소) ;
  • 소양강 (한국원자력연구원 첨단방사선연구소) ;
  • 진창현 (한국원자력연구원 첨단방사선연구소) ;
  • 변명우 (우송대학교 외식조리영양학부) ;
  • 김왕근 (기초과학연구원 중이온가속기구축사업단) ;
  • 정일윤 (한국원자력연구원 첨단방사선연구소)
  • Received : 2012.03.23
  • Accepted : 2012.04.19
  • Published : 2012.07.31
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to elucidate the anti-proliferative effect and the mechanisms underlying apoptosis induced by a methanol extracts from Ishige sinicola (ISE) in HeLa cells. ISE treatment for 24 hr significantly inhibited cell viability in a dose-dependent manner. Apoptosis was detected by Hoechst 33258 staining and an annexin V/PI assay after 24 hr treatment. Moreover, ISE treatment triggered the cleavage of caspase-8, -9, -3, and poly(ADP-ribose) polymerase (PARP) in dose-dependent and time-dependent manners. In addition, z-VAD-fmk, a general caspase inhibitor, blocked ISE-induced cell death. Taken together, these results suggest that ISE-induced apoptosis is mediated by the activation of a caspase cascade in HeLa cells.

본 연구에서는 넓패 메탄올 추출물의 농도별 처리가 인체 자궁암 세포 HeLa의 세포사멸에 미치는 영향을 확인하기 위하여 세포독성 측정, Hoechst 33258 staining, flow cytometry 분석을 통하여 세포사멸을 확인하였다. 넓패 메탄올 추출물 처리 시 HeLa 세포에서 농도 의존적으로 세포의 증식을 억제하였으며, 또한 넓패 메탄올 추출물은 농도 의존적으로 핵을 응축하고 apoptotic bodies을 생성하였다. 유세포 분석을 통하여 apoptosis를 측정한 결과, 넓패 메탄올 추출물의 농도가 증가함에 따라 유의적으로 apoptotic 세포가 증가하였다. Western blot을 통해 PARP 단백질의 절단 현상을 분석한 결과, 넓패 메탄올 추출물의 처리 농도와 시간에 따라 PARP 단백질의 절단 현상이 증가하였다. 또한 넓패 메탄올 추출물은 caspase-8, caspase-9 및 caspase-3 활성을 농도와 시간에 따라 증가시켰으며, caspase 저해제인 z-VAD-fmk로 처리 시 넓패 메탄올 추출물에 의한 세포사멸이 유의적으로 감소되어 넓패 메탄올 추출물에 의한 HeLa 세포의 apoptosis 유도에 caspase가 중요한 역할을 하고 있음을 확인하였다. 따라서 넓패 메탄올 추출물은 HeLa 자궁암 세포의 apoptosis를 유도하는 것으로 나타나 넓패의 항암효과 가능성을 제시하였다.

Keywords

References

  1. Kang HI, Kim JY, Cho HD, Park KW, Kang JS, Seo KI. 2010. Resveratrol induces apoptosis in primary human prostate cancer cells. J Korean Soc Food Sci Nutr 39: 1119-1125. https://doi.org/10.3746/jkfn.2010.39.8.1119
  2. Tan ML, Ooi JP, Ismail N, Moad AIH, Muhammad TST. 2009. Programmed cell death pathways and current antitumor targets. Pharm Res 26: 1547-1560. https://doi.org/10.1007/s11095-009-9895-1
  3. Nobili S, Lippi D, Witort E, Donnini M, Bausi L, Mini E, Capaccioli S. 2009. Natural compounds for cancer treatment and prevention. Pharmacol Res 59: 365-378. https://doi.org/10.1016/j.phrs.2009.01.017
  4. Chae YH, Shin DY, Park C, Lee YT, Moon SG, Choi YH. 2011. Induction of apoptosis in human colon carcinoma HCT116 cells using a water extract of Lepidium virginicum L. J Korean Soc Food Sci Nutr 40: 649-659. https://doi.org/10.3746/jkfn.2011.40.5.649
  5. Kim KN, Heo SJ, Kang SM, Ahn G, Jeon YJ. 2010. Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a ROS-mediated Bcl-xL pathway. Toxicol in Vitro 24: 1648-1654. https://doi.org/10.1016/j.tiv.2010.05.023
  6. Das SK, Ren R, Hashimoto T, Kanazawa K. 2010. Fucoxanthin induces apoptosis in osteoclast-like cells differentiated from RAW 264.7 cells. J Agric Food Chem 58: 6090-6095. https://doi.org/10.1021/jf100303k
  7. Athukorala Y, Lee KW, Kim SK, Jeon YJ. 2007. Anticoagulant activity of marine green and brown algae collected from Jeju Island in Korea. Bioresour Technol 98: 1711-1716. https://doi.org/10.1016/j.biortech.2006.07.034
  8. Athukorala Y, Lee KW, Kim SK, Jeon YJ. 2007. Anticoagulant activity of marine green and brown algae collected from Jeju Island in Korea. Bioresour Technol 98: 1711-1716. https://doi.org/10.1016/j.biortech.2006.07.034
  9. Heo SJ, Yoon WJ, Kim KN, Ahn GN, Kang SM, Kang DH, Affan A, Oh C, Jung WK, Jeon YJ. 2010. Evaluation of anti- inflammatory effect of fucoxanthin isolated from brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages. Food Chem Toxicol 48: 2045-2051. https://doi.org/10.1016/j.fct.2010.05.003
  10. Heo SJ, Park EJ, Lee KW, Jeon YJ. 2005. Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresour Technol 96: 1613-1623. https://doi.org/10.1016/j.biortech.2004.07.013
  11. Iwashima M, Mori J, Ting X, Matsunaga T, Hayashi K, Shinoda D, Saito H, Sankawa U, Hayashi T. 2005. Antioxidant and antiviral activities of plastoquinones from the brown alga Sargassum micracanthum, and a new chromene derivative converted from the plastoquinones. Biol Pharm Bull 28: 374-377. https://doi.org/10.1248/bpb.28.374
  12. Lee IK, Kim YH. 1999. Biodiversity and distribution of marine benthic organisms and uses of algal resources in the coastal zone of Korea and Japan I. Benthic marine algae in the East coast of Korea. Algae 14: 91-110.
  13. Heo SJ, Ko SC, Kang SM, Cha SH, Lee SH, Kang DH, Jung WK, Affan A, Oh CH, Jeon YJ. 2010. Inhibitory effect of diphlorethohydroxycarmalol on melanogenesis and its protective effect against UV-B radiation-induced cell damage. Food Chem Toxicol 48: 1355-1361. https://doi.org/10.1016/j.fct.2010.03.001
  14. Hirao S, Tara K, Kuwano K, Tanaka J, Ishibashi F. 2012. Algicidal activity of glycerolipids from brown alga Ishige sinicola toward red tide microalgae. Biosci Biotechnol Biochem 76: 372-374. https://doi.org/10.1271/bbb.110645
  15. Kim SA, Kim J, Woo MK, Kwak CS, Lee MS. 2005. Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweeds. J Korean Soc Food Sci Nutr 34: 451-459. https://doi.org/10.3746/jkfn.2005.34.4.451
  16. Ham YA, Choi HJ, Kim SH, Chung MJ, Ham SS. 2009. Antimutagenic and antitumor effects of Adenophora triphylla extracts. J Korean Soc Food Sci Nutr 38: 25-31. https://doi.org/10.3746/jkfn.2009.38.1.025
  17. Gupta S, Abu-Ghannam N. 2011. Bioactive potential and possible health effects of edible brown seaweeds. Trends Food Sci Tech 22: 315-326. https://doi.org/10.1016/j.tifs.2011.03.011
  18. Zou Y, Qian ZJ, Li Y, Kim MM, Lee SH, Kim SK. 2008. Antioxidant effects of phlorotannins isolated from Ishige okamurae in free radical mediated oxidative systems. J Agric Food Chem 56: 7001-7009. https://doi.org/10.1021/jf801133h

Cited by

  1. Chemical Composition and Antiproliferative Activity of Supercritical Extract of Immature Citrus Peel in human cervical carcinoma HeLa cells vol.16, pp.12, 2015, https://doi.org/10.5762/KAIS.2015.16.12.8836
  2. LPS로 유도된 RAW 264.7 Cell과 마우스 모델에 대한 넓패(Ishige sinicola) 에탄올 추출물의 항염증 효과 vol.24, pp.8, 2012, https://doi.org/10.11002/kjfp.2017.24.8.1149