Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Analysis of Anti-adipogenic Constituents of Cordyceps militaris Using High Performance Liquid Chromatography-Diode Array Detection in Different Samples: Comparison with Anti-adipogenic Activity

  • Received : 2012.05.31
  • Accepted : 2012.07.26
  • Published : 2012.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

We previously isolated cordycepin, guanosine and tryptophan from Cordyceps militaris as antiadipogenic constituents. For the quality control of C. militaris for anti-adipogenic activity, simultaneous analytical method using high-performance liquid chromatography (HPLC)-diode array detection (DAD) was developed and validated. Quantitation of these compounds in various Cordyceps samples from different sources and various extraction methods were conducted using developed method. Our study shows that natural Cordyceps and host insect possess higher content than cultured ones and fruiting bodies, respectively. The content of cordycepin showed great difference in different C. militaris samples whereas trytophan content was similar in tested samples. Addition of water to extraction solvent greatly increased the yield of guanosine and tryptophan. High temperature and longer extraction time increased yield of guanosine, whereas the content of trytophan was decreased in high temperature during extraction with water. Extraction using ultrasonic apparatus slightly increased extraction efficiency. Cordycepin, however, has little variation in different extraction method tested. Strong anti-adipogenic activity was observed in the samples that contain all the three constituents. Taken together, quantitation of these compounds using developed analytical method might provide basic requirement for the anti-adipogenic activity of C. militaris.

Keywords

References

  1. Choi, I.Y., Choi, J.S., Lee, W.H., Yu, Y.J., Joung, G.T., Ju, I.O., and Choi, Y.K., The condition of production of artificial fruiting body of Cordyceps militaris. Kor. J. Mycol. 27, 243-248 (1999).
  2. Hong, I.P., Kang, P.D., Kim, K.Y., Nam, S.H., Lee, M.Y., Choi, Y.S., Kim, N.S., Kim, H.K., Lee, K.G., and Humber, R.A., Fruit body formation on silkworm by Cordycep smilitaris. Mycobiology 38, 128-132 (2010). https://doi.org/10.4489/MYCO.2010.38.2.128
  3. Kim, H.S., Kim, J.Y., Kang, J.S., Kim, H.M., Kim, Y.O., Hong, I.P., Lee, M.K., Hong, J.T., Kim, Y., and Han, S.-B., Cordlan polysaccharide isolated from mushroom Cordyceps militaris induces dendritic cell maturation through toll-like receptor 4 signaling. Food Chem. Toxicol. 48, 1926-1933 (2010). https://doi.org/10.1016/j.fct.2010.04.036
  4. Koh, J.H., Kim, K.M., Kim, J.M., Song, J.C., and Suh, H.J., Antifatigue and antistress effect of the hot-water fraction from mycelia of Cordyceps sinensis. Biol. Pharm. Bull. 26, 691-694 (2003). https://doi.org/10.1248/bpb.26.691
  5. Lee, H., Kim, Y.J., Kim, H.W., Lee, D.H., Sung, M.K., and Park, T., Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells. Biol. Pharm. Bull. 29, 670-674 (2006). https://doi.org/10.1248/bpb.29.670
  6. Li, S.P., Yang, F.Q., and Tsim, K.W.K., Quality control of Cordyceps sinensis, a valued traditional Chinese medicine. J. Pharm. Biomed. Anal. 41, 1571-1584 (2006). https://doi.org/10.1016/j.jpba.2006.01.046
  7. Liu, Q., Hong, I.P., Ahn, M.-J., Yoo, H.-S., Han, S.-B., Hwang, B.Y., and Lee, M.K., Anti-adipogenic activity of Cordyceps militaris in 3T3-L1 cells. Nat. Prod. Comm. 6, 1839-1841 (2011).
  8. Paterson, R.R.M. Cordyceps - A traditional Chinese medicine and another fungal therapeutic biofactory? Phytochemisty 69, 1469-1495 (2008). https://doi.org/10.1016/j.phytochem.2008.01.027
  9. Shimada, T., Hiramatsu, N., Kasai, A., Mukai, M., Okamura, M., Yao, J., Huang, T., Tamai, M., Takahashi, S., Nakamura, T., and Kitamura, M., Suppression of adipocyte differentiation by Cordyceps militaris through activation of the aryl hydrocarbon receptor. Am. J. Physiol. Endocrinol. Metab. 295, E859-867 (2008). https://doi.org/10.1152/ajpendo.90373.2008
  10. Shin, E.J., Choi, K.-M., Yoo, H.-S., Lee, C.-K., Hwang, B.Y., and Lee, M.K., Inhibitory effects of coumarins from the stem barks of Fraxinus rhynchophylla on adipocyte differentiation in 3T3-L1 cells. Biol. Pharm. Bull. 33, 1610-1614 (2010). https://doi.org/10.1248/bpb.33.1610
  11. Sung, J.M., Choi, Y.S., Shrestha, B., and Park, Y.J., Investigation on artificial fruiting of Cordyceps militaris. Kor. J. Mycol. 30, 6-10 (2002). https://doi.org/10.4489/KJM.2002.30.1.006
  12. Yang, F.Q., Feng, K., Zhao, J., and Li, S.P., Analysis of sterols and fatty acids in natural and cultured Cordyceps by one-step derivatization followed with gas chromatography-mass spectrometry. J. Pharm. Biomed. Anal. 49, 1172-1178 (2009). https://doi.org/10.1016/j.jpba.2009.02.025
  13. Yang, F.Q., Ge, L., Yong, J.W.H., Tan, S.N., and Li, S.P., Determination of nucleosides and nucleobases in different species of Cordyceps by capillary electophoresis-mass spectrometry. J. Pharm. Biomed. Anal. 50, 307-314 (2009). https://doi.org/10.1016/j.jpba.2009.04.027
  14. Yu, R., Ye, B., Yan, C., Song, L., Zhang, Z., Wang, W., and Zhao, Y., Fingerprint analysis of fruiting bodies of cultured Cordyceps militaris by high-performance liquid chromatography-photodiode array detection. J. Pharm. Biomed. Anal. 44, 818-823 (2007). https://doi.org/10.1016/j.jpba.2007.03.024
  15. Yun, Y., Han, S., Lee, S., Ko, S., Lee, C., Ha, N., and Kim, K., Antidiabetic effects of CCCA, cmESS, and cordycepin from Cordyceps militaris and the immune responses in streptozotocin-induced diabetic mice. Nat. Prod. Sci. 9, 291-298 (2006).