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Effects of CuEDTA and FeEDTA Foliar Spray on Antioxidant Activities of Apple

CuEDTA와 FeEDTA의 엽면살포가 사과의 항산화 활성에 미치는 영향

  • Park, Ji-Young (Dept. of Horticultural Science, Chungbuk National University) ;
  • Ryu, Ho-Ung (Dept. of Horticultural Science, Chungbuk National University) ;
  • Shin, Hyun-Suk (Dept. of Horticultural Science, Chungbuk National University) ;
  • Lim, Heon-Kyu (Dept. of Horticultural Science, Chungbuk National University) ;
  • Son, In-Chang (Agricultural Research Center for Climate Change, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Dae-Il (Dept. of Horticultural Science, Chungbuk National University) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University)
  • 박지영 (충북대학교 원예과학과) ;
  • 류호웅 (충북대학교 원예과학과) ;
  • 신현석 (충북대학교 원예과학과) ;
  • 임헌규 (충북대학교 원예과학과) ;
  • 손인창 (국립원예특작과학원 온난화대응농업연구센터) ;
  • 김대일 (충북대학교 원예과학과) ;
  • 정헌상 (충북대학교 식품공학과) ;
  • 이준수 (충북대학교 식품공학과)
  • Received : 2012.05.21
  • Accepted : 2012.08.10
  • Published : 2012.09.30

Abstract

For functional enhancement of apples, the effects of CuEDTA and FeEDTA foliar spray were investigated on the antioxidant contents and antioxidant activities in 'Hongro' fruit, which is a representative early season harvesting apple cultivar, at 30 days before harvest. The polyphenolic content of peel was significantly higher in the CuEDTA (1,228.6 mg/100 g) and FeEDTA (1,210.0 mg/100 g) spraying treatment groups compared to the control group (998.8 mg/100 g). The flavonoid content of peel showed the same trend as that of polyphenolic content. The ascorbic acid content of peel as also significantly increased in the CuEDTA and FeEDTA spraying treatment groups, but anthocyanin content was the highest in the control group (560.6 mg/100 g). The ABTS and DPPH radical scavenging activities were higher in the CuEDTA and FeEDTA spraying treatment groups than in the control group, but reducing power was not significantly different between the treatments. As a result of this study, 3% CuEDTA and FeEDTA spraying treatments at 30 days before harvest can be used to effectively enhance antioxidant contents in 'Hongro' apple.

본 연구는 수확기 30일 전 CuEDTA, FeEDTA 엽면살포가 '홍로' 사과 과실의 항산화 성분 및 활성에 미치는 영향에 대해 조사하였다. 메탄올 추출물을 이용하여 항산화물질의 함량을 측정한 결과, 과피의 폴리페놀은 CuEDTA와 FeEDTA에서 1,228.6, 1,210.0 mg/100 g으로 대조구의 998.8 mg/100g보다 높은 수치를 나타내었고, 플라보노이드 함량도 CuEDTA, FeEDTA가 988.5, 847.6 mg/100 g으로 대조구 665.7 mg/100 g보다 높게 나타났다. Ascorbic acid의 함량은 FeEDTA가 47.2 mg/100 g으로 가장 높았고 CuEDTA(44.8 mg/100 g), 대조구( 40.9 mg/100 g) 순이었다. 안토시아닌은 대조구가 560.6 mg/100 g으로 CuEDTA, FeEDTA의 544.6, 542.9 mg/100 g에 비해 높았다. 항산화 활성 분석 결과, 과육은 처리구별 항산화 활성이 유의성이 인정되지 않았으나(p>0.05), 과피에서는 처리에 따라 유의적인 차이를 보였다(p<0.05). ABTS radical을 이용한 과피의 총 항산화력은 FeEDTA(1,267.5), CuEDTA(1,224.9), 대조구(1,161.1) 순으로 높은 활성을 보였으며, DPPH radical 소거능 또한 대조구의 868.7 mg AEAC/100 g에 비해 FeEDTA, CuEDTA 처리구에서 1,048.8, 1,042.7 mg AEAC/100 g으로 높았다. 하지만 환원력은 처리구간 유의성이 인정되지 않았다(p>0.05).

Keywords

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