The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification of Orchid Mycorrhizal Fungi Isolated from Five Species of Terrestrial Orchids in Korea

한국에 자생하는 5종의 지생란에서 분리한 난균근균의 동정

  • Youm, Jae-Young (Department of Biology, Korea National University of Education) ;
  • Han, Han-Kyeol (Department of Biology, Korea National University of Education) ;
  • Chung, Jae-Min (Department of Plant Resources Conservation, Korea National Arboretum) ;
  • Cho, Yong-Chan (Department of Plant Resources Conservation, Korea National Arboretum) ;
  • Lee, Byung-Chun (Department of Plant Resources Conservation, Korea National Arboretum) ;
  • Eom, Ahn-Heum (Department of Biology, Korea National University of Education)
  • 염재영 (한국교원대학교 생물교육과) ;
  • 한한결 (한국교원대학교 생물교육과) ;
  • 정재민 (국립수목원 산림자원보존과) ;
  • 조용찬 (국립수목원 산림자원보존과) ;
  • 이병천 (국립수목원 산림자원보존과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2012.07.25
  • Accepted : 2012.09.08
  • Published : 2012.09.28
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Abstract

This study was performed to isolate and identify the orchid mycorrhizal fungi (OMF) from roots of five species of terrestrial orchids in Korea; Cymbidium goeringii, Spiranthes sinensis, Calanthe discolor, Bletilla striata, Pogonia minor. DNA was extracted from isolated OMF and ITS region was amplified using primers, ITS1-OF and ITS4-OF. Four species of OMF belong to Tulasnellaceae and Sebacinaceae were identified; Tulasnella calospora, Tulasnella irregularis, Tulasnella sp., Sebacina vermifera.

제주도와 완도에서 채집한 총 5종의 자생란의 뿌리에서 미동정 1종을 포함한 총 4종의 균을 순수분리하였으며, 추출한 DNA를 ITS지역을 증폭하여 분자적 동정하였다. 이중 3종이 Tulasnella속이었으며, 1종은 Sebacina vermifera로 일반적인 난균근균이었다. 제주도와 완도에서 모두 채집한 보춘화(Cymbidium goeringii)의 경우 제주도에서 채집한 뿌리에서는 Sebacina vermifera가, 완도에서 채집한 뿌리에서는 Tulasnella calospora가 분리되어 같은 종의난과 식물에서도 서식지에 따라 공생하는 난균근균이 다를 수 있음을 확인하였다.

Keywords

References

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