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한국에 자생하는 5종의 지생란에서 분리한 난균근균의 동정

Identification of Orchid Mycorrhizal Fungi Isolated from Five Species of Terrestrial Orchids in Korea

  • 염재영 (한국교원대학교 생물교육과) ;
  • 한한결 (한국교원대학교 생물교육과) ;
  • 정재민 (국립수목원 산림자원보존과) ;
  • 조용찬 (국립수목원 산림자원보존과) ;
  • 이병천 (국립수목원 산림자원보존과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Youm, Jae-Young (Department of Biology, Korea National University of Education) ;
  • Han, Han-Kyeol (Department of Biology, Korea National University of Education) ;
  • Chung, Jae-Min (Department of Plant Resources Conservation, Korea National Arboretum) ;
  • Cho, Yong-Chan (Department of Plant Resources Conservation, Korea National Arboretum) ;
  • Lee, Byung-Chun (Department of Plant Resources Conservation, Korea National Arboretum) ;
  • Eom, Ahn-Heum (Department of Biology, Korea National University of Education)
  • 투고 : 2012.07.25
  • 심사 : 2012.09.08
  • 발행 : 2012.09.28

초록

제주도와 완도에서 채집한 총 5종의 자생란의 뿌리에서 미동정 1종을 포함한 총 4종의 균을 순수분리하였으며, 추출한 DNA를 ITS지역을 증폭하여 분자적 동정하였다. 이중 3종이 Tulasnella속이었으며, 1종은 Sebacina vermifera로 일반적인 난균근균이었다. 제주도와 완도에서 모두 채집한 보춘화(Cymbidium goeringii)의 경우 제주도에서 채집한 뿌리에서는 Sebacina vermifera가, 완도에서 채집한 뿌리에서는 Tulasnella calospora가 분리되어 같은 종의난과 식물에서도 서식지에 따라 공생하는 난균근균이 다를 수 있음을 확인하였다.

This study was performed to isolate and identify the orchid mycorrhizal fungi (OMF) from roots of five species of terrestrial orchids in Korea; Cymbidium goeringii, Spiranthes sinensis, Calanthe discolor, Bletilla striata, Pogonia minor. DNA was extracted from isolated OMF and ITS region was amplified using primers, ITS1-OF and ITS4-OF. Four species of OMF belong to Tulasnellaceae and Sebacinaceae were identified; Tulasnella calospora, Tulasnella irregularis, Tulasnella sp., Sebacina vermifera.

키워드

참고문헌

  1. Cribb, P. J., Lell, S. P., Dixon, K. W. and Barrett R. L. 2003. Orchid conservation: a global perspective. In: Orchid Conservation, pp. 1-24. Eds. K. W. Dixon, S. P. Kell, R. L. Barrett and P. J. Cribb. Natural History Publication, Kota Kinabalu, Malaysia.
  2. Dearnaley, J. D. 2007. Further advances in orchid mycorrhizal research. Mycorrhiza 17:475-486. https://doi.org/10.1007/s00572-007-0138-1
  3. Kim, S. N. and Lee, K. S. 1997. Orchids in Korea. Kyohaksa, Seoul. 495p. (in Korean).
  4. Lee, J. K., Lee, S. S., Eom, A. H. and Paek, K. Y. 2003. Interactions of newly isolated orchid mycorrhizal fungi with Korean Cymbidium kanran hybrid 'Chungsu'. Mycobiology 31:151-156. https://doi.org/10.4489/MYCO.2003.31.3.151
  5. Liu, H., Luo, Y. and Liu, H. 2010. Studies of mycorrhizal fungi of Chinese orchids and their role in orchid conservation in China-a review. Bot. Rev. 76:241-262. https://doi.org/10.1007/s12229-010-9045-9
  6. Nontachaiyapoom, S., Sasirat, S. and Manoch, L. 2010. Isolation and identification of Rhizoctonia-like fungi from roots of three orchid genera, Paphiopedilum, Dendrobium, and Cymbidium, collected in Chiang Rai and Chiang Maiprovinces of Thailand. Mycorrhiza 20: 459-471. https://doi.org/10.1007/s00572-010-0297-3
  7. Peterson, R. L., Uetake, Y. and Zelmer, C. 1998. Fungal symbioses with orchid protocorms. Symbiosis. 25:29-55.
  8. Smith, S. E. and Read, D. 2008. Mycorrhizal Symbiosis, 3rd edition, pp. 419-457. Academic Press, New York.
  9. Sneh, B., Burpee, L. and Ogoshi, A. 1991. Identification of Rhizoctonia species. APS Press. St. Paul, MN, USA. 133p.
  10. Taylor, D. L. and McCormick, M. K. 2008. Internal transcribed spacer primers and sequences for improved characterization of basidiomycetous orchid mycorrhizas. New Phytol. 177:1020-1033. https://doi.org/10.1111/j.1469-8137.2007.02320.x
  11. Youm, J. Y., Chung, J. M., Lee, B. C. and Eom, A. H. 2011. Molecular identification of orchid mycorrhizal fungi of native orchids in Ulleung Island. Kor. J. Mycol. 39:7-10. (in Korean). https://doi.org/10.4489/KJM.2011.39.1.0007
  12. Zhao, J. and Liu, H. 2008. Effects of fungal elicitors on the protocorm of Cymbidium eburneum. Ecol. Sci. 27:134-137.

피인용 문헌

  1. Antioxidant Activity and Inhibitory Activities of Xanthine Oxidase and Tyrosinase of Yeasts from Wild Flowers in Korea vol.43, pp.2, 2015, https://doi.org/10.4489/KJM.2015.43.2.129
  2. Spiranthes igniorchis (Orchidaceae), a new and rare cryptic species from the south-central Florida subtropical grasslands vol.69, pp.3, 2017, https://doi.org/10.1007/s12228-017-9483-3
  3. Identification of Orchid Mycorrhizal Fungi Isolated from Epipactis thunbergii in Korea vol.41, pp.1, 2013, https://doi.org/10.4489/KJM.2013.41.1.9
  4. Collected from Different Sites in Korea vol.45, pp.4, 2017, https://doi.org/10.5941/MYCO.2017.45.4.312
  5. Diversity of fungi associated with roots of Calanthe orchid species in Korea vol.56, pp.1, 2018, https://doi.org/10.1007/s12275-018-7319-9