The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Production of Lignocellulytic Enzymes from Spent Mushroom Compost of Pleurotus eryngii

큰느타리버섯 수확 후 배지로부터 리그닌섬유소분해효소 생산

  • Lim, Sun-Hwa (Graduate School of Bio. & Information Technology, Hankyong National University) ;
  • Kim, Jong-Kun (Graduate School of Bio. & Information Technology, Hankyong National University) ;
  • Lee, Yun-Hae (Mushroom Research Institute, GARES) ;
  • Kang, Hee-Wan (Graduate School of Bio. & Information Technology, Hankyong National University)
  • 임선화 (한경대학교 바이오.정보기술 전문대학원) ;
  • 김종군 (한경대학교 바이오.정보기술 전문대학원) ;
  • 이윤혜 (경기도농업기술원 버섯연구소) ;
  • 강희완 (한경대학교 바이오.정보기술 전문대학원)
  • Received : 2012.09.10
  • Accepted : 2012.09.16
  • Published : 2012.09.28
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Abstract

The lignocellulytic enzymes including a-amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), ${\beta}$-xylosidase (EC 3.2.1.37), ${\beta}$-glucosidase (EC 3.2.1.21) and cellulase (EC 3.2.1.4) were extracted from spent mushroom compost (SMC) of Pleurotus eryngii. Different extraction buffers and conditions were tested for optimal recovery of the enzymes. The optimum extraction was shaking incubation (200 rpm) for 2 h at $4^{\circ}C$. ${\alpha}$-Amylase was extracted with the productivity range from 1.20 to 1.6 Unit/SMC g. Cellulase was recovered with the productivity range from 2.10 to 2.80 U/gf. ${\beta}$-glucosidase and ${\beta}$-xylosidase productivities showed lowest recovery producing 0.1 U/g and 0.02 U/g, respectively. The P. eryngii SMCs collected from three different mushroom farms showed different recovery on laccase and xylanse, cellulase. Furthermore, the water extracted SMC was compared to commercial enzymes for its industrial application in decolorization and cellulase activity.

큰느타리 수확 후 배지(spent mushroom compost, SMC)로부터 목질분해효소인 ${\alpha}$-amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), ${\beta}$-xylosidase (EC 3.2.1.37), ${\beta}$-glucosidase (EC 3.2.1.21) cellulase (EC 3.2.1.4)가 다양한 buffer로 추출 되었으며 1 g SMC당 5 volume으로 첨가하고 2시간 동안 $4^{\circ}C$에서 200 rpm속도로 진탕배양 시에 최적 효소회수율을 보였다. ${\alpha}$-Amylase는 2.10에서 2.80 U/g (SMC)의 효소활성을 보였으며 ${\beta}$-glucosidase와 ${\beta}$-xylosidase는 0.1 U/g 이하의 가장 낮은 효소활성이 나타났다. Cellulase는 2.80 U/g와 xylanse는 5.0 U/g이상의 비교적 높은 효소회수율을 보였다. 큰느타리버섯 SMC 추출물은 상업용 laccase와 탈색효과 cellulase와는 filter paper분해활성을 비교하여 산업적 적용을 평가하였다.

Keywords

References

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