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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Physiological roles of N-acetylglucosaminyltransferase V (GnT-V) in mice

  • Miyoshi, Eiji (Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine) ;
  • Terao, Mika (Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine) ;
  • Kamada, Yoshihiro (Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine)
  • Received : 2012.09.15
  • Published : 2012.10.31
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Abstract

Oligosaccharide modification by N-acetylglucosaminyltransferase-V (GnT-V), a glycosyltransferase encoded by the Mgat5 gene that catalyzes the formation of ${\beta}1$,6GlcNAc (N-acetylglucosamine) branches on N-glycans, is thought to be associated with cancer growth and metastasis. Overexpression of GnT-V in cancer cells enhances the signaling of growth factors such as epidermal growth factor by increasing galectin-3 binding to polylactosamine structures on receptor N-glycans. In contrast, GnT-V deficient mice are born healthy and lack ${\beta}1$,6GlcNAc branches on N-glycans, but develop immunological disorders due to T-cell dysfunction at 12-20 months of age. We have developed Mgat5 transgenic (Tg) mice (GnT-V Tg mice) using a ${\beta}$-actin promoter and found characteristic phenotypes in skin, liver, and T cells in the mice. Although the GnT-V Tg mice do not develop spontaneous cancers in any organs, there are differences in the response to external stimuli between wild-type and GnT-V Tg mice. These changes are similar to those seen in cancer progression but are unexpected in some aspects. In this review, we summarize what is known about GnT-V functions in skin and liver cells as a means to understand the physiological roles of GnT-V in mice.

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References

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