Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Physicochemical Characteristics and Antioxidant activity, Antimutagenicity, and Cytotoxicity of Hot-water Extract of Hericium erinaceus

노루궁뎅이 버섯 열수 추출물의 이화학적 특성 및 항산화성, 항돌연변이성, cytotoxicity 분석

  • Kim, Se Ryung (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Meera (Dept. of Food Science and Nutrition, Kyungpook National University.Center for Beautiful Aging)
  • 김세령 (경북대학교 식품영양학과) ;
  • 김미라 (경북대학교 식품영양학과.장수생활과학연구소)
  • Published : 2012.10.31
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Abstract

The physicochemical characteristics and biological activities, including antioxidant activity, antimutagenicity, and cytotoxicity of hot-water extract of fruiting body of Hericium erinaceus, were investigated in this study. Hot-water extract of fruiting body of Hericium erinaceus contained carbohydrate (7.86%), protein (10.91%), and ${\beta}$-glucan (3.62%). Water solubility of hot-water extract was 42.58%. Antioxidant activities of the extract were evaluated by ABTS assay and FRAP assay. The $IC_{50}$ value was 312.21 ${\mu}g/mL$ in ABTS assay. Antimutagenic activity of the extract was evaluated by Ames test. Antimutagenicity of hot-water extract (5 mg/mL) on Salmonella Typhimurium TA100 mutagenated by sodium azide (0.15 ${\mu}g/mg$) was 69.2%. Cytotoxicity of hot-water extract was also evaluated by MTT and SRB assay. The cytotoxicity was highest (83.95%) on Hep3B treated with 2,000 ${\mu}g/mL$ of hot-water extract in SRB assay. Therefore, it is suggested that hot-water extract of fruiting body of Hericium erinaceus has high antioxidant activity, antimutagenicity, and cytotoxicity.

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References

  1. 농촌진흥청 농업과학기술원. 2004. 한국의 버섯-식용버섯과 독버섯. 동방미디어. 서울. pp 264-265
  2. 성낙계, 성낙주, 정영철. 2005. 암을 이기는 신비의 상황버섯 다당체. 북랜드. 서울. pp 170-187
  3. Benzie F, Strain J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power" :The FRAP assay. J Anal Bio Chem 239(1):70-76
  4. Bradford MM. 1976. A rapid, sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biol Chem 72:248-254
  5. Carmichael J, Degraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Can Res 47:936-942
  6. Chang PS, Cho GB. 1997. Oxidation of primary alcohol groups of polysaccharides with 2,2,6,6-Tetramethyl-1-piperidine oxoammonium ion. Korean J Food Sci Technol 29(3):446-451
  7. Choi MA, Park NY, Woo SM, Jeong YJ, Shin SR. 2003. Characteristics of Hericium erinaceus and its extracts. Korean J Food Preserv 10(4):560-564
  8. Doll R, Peto R. 1981. The causes of cancer: quantitative estimates of avoidable risks of cancer in the United States today. J Cancer Inst 66(6):1191-1308.
  9. Hong JH, Youn KS, Choi YH. 2004. Characteristics of crude protein-bound polysaccharide from Agaricus blasei Murill by extraction and precipitation conditions and its antitumor effect. Korean J Food Sci Technol 36(4):586-593
  10. Jang HY. 2005. Antioxidative activities and chemopreventive effects of Hericium erinaceus extracts. MA thesis. Keimyung University of Korea. p 16
  11. Jung EB, Jo JH, Cho SM. 2008. Nutritional component and anticancer properties of various extracts from haesongi mushroom (Hypsizigus marmoreus). J Korean Soc Food Sci Nutr 37(11):1395-1400 https://doi.org/10.3746/jkfn.2008.37.11.1395
  12. Jung HY. 2009. Physicochemical properties and biological activities of polysaccharide from Pleurotus eryngii and its derivatives. MA. Thesis. Hanyang University
  13. Jung IC, Park S, Park KS, Ha HC, Kim SH, Kwon YI, Lee JS. 1996. antioxidative effect of fruit body and mycelial extracts of Pleurotus ostreatus. Korean J Food Sci Technol 28(3):464-469
  14. Kawagishi H, Shimada A, Hosokawa S, Mori H, Sakamoto H, Ishiguro, Sakemi S, Bordner J, Kojima N, Furukawa S. 1996. Erinacines E, F and G stimulators of nerve growth factor (NGF)-synthesis from the mycelia of Hericium erinaceum. Tetrahedron Lett 37:7399-7402 https://doi.org/10.1016/0040-4039(96)01687-5
  15. Keong CY, Rashid BAA, Ing YS, Ismail Z. 2007. Quantification and identification of polysaccharide contents in Hericium erinaceus. Nutr Food Sci 37(4):260-271 https://doi.org/10.1108/00346650710774631
  16. Kim HJ, Lee BH, Lee KD, Lee KR. 1998. Screening for antimutagenic effects of the wild mushrooms in Korea. Korean J Food Sci Technol 30(3):688-692
  17. Kim JO, Jung MJ, Choi HJ, Lee JT, Lim AK, Hong JH, Kim DI. 2008. Antioxidative and biological activity of hot water and ethanol extracts from Phellinus linteus. J Korean Soc Food Sci Nutr 37(6):684-690 https://doi.org/10.3746/jkfn.2008.37.6.684
  18. Kim SP, Choi YH, Kang MY, Nam SH. 2005. Effects of the extracts by extraction procedures from Hericium erinaceus on activation of macrophase. J Korean Agr Chem Soc 48(3):285-291
  19. Kim YE, Yang JW, Lee CH, Kwon EK. 2009. ABTS radical scavenging and anti-tumor effects of Thricholoma matsutake sing (Pine mushroom). J Korean Soc Food Sci Nutr 38(5):555-560 https://doi.org/10.3746/jkfn.2009.38.5.555
  20. Lee HI. 2004. (The) study on the immune modulatory activity of Hericium-erinaceum water extract. MA Thesis. KonKuk University of Korea. pp 8-21
  21. Lee JS, Cho JY, Hong EK. 2009. Study on marcophage activation and structural characteristics of purified polysaccharides from the liquid culture broth of Hericium erinaceus. Carbohydr Polym 78:162-168 https://doi.org/10.1016/j.carbpol.2009.04.036
  22. Lee SO, Kim MJ, Kim DG, Choi HJ. 2005. Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquus. J Korean Soc Food Sci Nutr 34(2):139-147 https://doi.org/10.3746/jkfn.2005.34.2.139
  23. Maron MI, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat Res 113:173-215. https://doi.org/10.1016/0165-1161(83)90010-9
  24. Masuko T, Minami A, Iwasaki N, Majima T, Nishimura SI, Lee YC. 2005. Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Anal Biochem 339:69-72 https://doi.org/10.1016/j.ab.2004.12.001
  25. Mizuno T. 1995. Bioactive substances in Yamabushitage, the Hericium erinaceum fungus, and its medicinal utilization. Food Rev Int 11(1):173-178 https://doi.org/10.1080/87559129509541027
  26. Park JM, Lee SH, Kim JO, Park HJ, Park JB, Sin JI. 2004. In vitro and in vivo effects of extracts of Lentinuns edodes on tumor growth in a human papillomavirus 16 oncogenes-transformed animal tumor model-apoptosis-mediated tumor cell growth inhibition. Korean J Food Sci Technol 36(1):141-146
  27. Park SH, Chang JS, Lee KR. 2003. Effect of Hericium erinaceus extract on cancer cell growth and expression of cell cycle associated proteins. J Korean Soc Food Sci Nutr 32(6):931-936 https://doi.org/10.3746/jkfn.2003.32.6.931
  28. Park SH, Kim JY, Chang JS, Oh EJ, Kim OM, Bae JT, Kim HJ, Hae DJ, Lee KR. 2001a. Protective effect of Hericium erinaceus extracts on hepatic injury induced by benzo($\alpha$)pyrene in mice. J Korean Soc Food Sci Nutr 30(5):928-932
  29. Park SH, Kim OM, Lee KR. 2001b. Antimutagenic and quinone reductase inducing activities of Hericium erinaceus extracts. J Korean Soc Food Sci Nutr 30(6):1287-1292
  30. Park SJ, Hong JH, Youn KS, Choi YH. 2006. Form manufacturing and quality characteristics using extracts from Hericium erinaceus. Korean J Food preservation 13(5):569-573
  31. Park WR. 2008. Studies on the antioxidant activity and preparation of the fermented Artemisia iwayomogi with functional mushroom. MA Thesis. Chungju National University of Korea. pp 31-33
  32. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-evans C. 1999. antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26(9):1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  33. Ryu SR, Lee WY, Ka KH. 2009. Comparative study on the wawdust cultivation and the antioxidants of Hericium spp. Korean J Mycology 37(1):80-85 https://doi.org/10.4489/KJM.2009.37.1.080
  34. Surenjav U, Zhang L, Xu X, Zhang X Zeng F. 2006. Effect of molecular structure on antitumor activities of (1$\rightarrow$3)-$\beta$-D-glucans from different Lentimus edodes. Carbohydr Polym 63(1):97-104 https://doi.org/10.1016/j.carbpol.2005.08.011
  35. Tao Y, Zhang L, Cheung C.K. 2006. Physicochemical properties and antitumor activities of water-soluble native and sulfated hyperbranched mushroom polysaccharides. Carbohydr Res 341:2261-2269 https://doi.org/10.1016/j.carres.2006.05.024
  36. Wasser SP. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol. 60(3): 258-274 https://doi.org/10.1007/s00253-002-1076-7
  37. Xu XM, Jun JY, Jeong IH. 2007. A study on the antioxidant activity of hae-songi mushroom (Hypsizigus marmoreus) hot water extracts. J Korean Soc Food Sci Nutr 36(11):1351-1357 https://doi.org/10.3746/jkfn.2007.36.11.1351

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