The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Effect of Heat Treatment on the Antioxidant Properties of Yacon (Smallanthus sonchifolius)

열처리에 따른 야콘의 항산화 활성 변화

  • Hwang, In Guk (Dept. of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Kim, Ha Yun (Dept. of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Park, Bo Ram (Dept. of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Han, Hye Min (Dept. of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Yoo, Seon Mi (Dept. of Agrofood Resources, National Academy of Agricultural Science, RDA)
  • 황인국 (국립농업과학원 농식품자원부) ;
  • 김하윤 (국립농업과학원 농식품자원부) ;
  • 박보람 (국립농업과학원 농식품자원부) ;
  • 한혜민 (국립농업과학원 농식품자원부) ;
  • 유선미 (국립농업과학원 농식품자원부)
  • Received : 2013.10.15
  • Accepted : 2013.12.05
  • Published : 2013.12.31
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Abstract

This study was investigated the effects of heat treatment on polyphenolic compounds and antioxidant activities of yacon. Raw yacon was heated at $100^{\circ}C$ and $121^{\circ}C$ for 15, 30, or 60 min by using an autoclave. The browning intensity, levels of free and bound phenolic and flavonoid compounds, and 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) radical-scavenging activity of yacon extracts following heat treatment were measured. The browning index, free polyphenolic content, and antioxidant activities in the extracts were significantly increased (p<0.05) with both increased heating temperature and time, while bound polyphenolic levels were decreased. The levels of free phenolic and flavonoid compounds in the yacon extract heated to $121^{\circ}C$ for 60 min were increased by 1.2 and 1.1 folds compared to raw yacon respectively. Moreover, DPPH and ABTS radical-scavenging activities of the yacon heated to $121^{\circ}C$ for 60 min were 1.7 fold and 2.0 fold higher, respectively, than those of raw yacon. A significant (p<0.01) correlation was observed among browning intensity, free and bound phenolic and flavonoid levels, and DPPH and ABTS radical-scavenging activities in heated yacon extract. Thus, heat treatment can be used as a method to enhance the antioxidant compound content in and the antioxidant activity of yacon.

본 연구에서는 열처리가 야콘의 항산화 활성에 미치는 영향을 살펴보기 위하여 열처리 온도(100 및 $121^{\circ}C$)와 시간(15, 30 및 60분)에 따른 항산화 성분 및 항산화 활성 변화를 조사하였다. 열처리 후 야콘의 갈변도, 유리형 및 결합형 폴리페놀 함량, 유리형 및 결합형 플라보노이드 함량, DPPH radical 소거 활성 및 ABTS radical 소거 활성을 측정하였다. 열처리 후 야콘의 갈변도, 유리형 폴리페놀 및 플라보노이드 함량과 항산화 활성은 열처리 온도와 시간에 따라 유의적(p<0.05)으로 증가하였고, 결합형 폴리페놀 및 플라보노이드 함량은 감소하였다. 야콘의 유리형 폴리페놀 및 플라보노이드 함량은 $121^{\circ}C$, 60분 열처리 시 생야콘에 비해 각각 1.2배 및 1.1배로 유의적(p<0.05)으로 증가하였다. 또한, 야콘의 DPPH radical 소거 활성 및 ABTS Radical 소거 활성도 $121^{\circ}C$, 60분 열처리 시 생야콘에 비해 각각 1.7배 및 2.0배로 유의적(p<0.05)으로 증가였다. 야콘의 갈변도, 폴리페놀 및 플라보노이드 함량과 DPPH radical 및 ABTS radical 소거 활성 간의 상관관계를 분석한 결과, 높은 상관관계(p<0.01)가 있는 것으로 나타났다. 본 연구결과, 열처리 방법을 통해 야콘의 항산화 성분과 항산화 활성을 강화시킬 수 있으며, 이를 활용한 기능성 식품 소재 개발이 가능할 것으로 생각된다.

Keywords

References

  1. Adefegha SA, Oboh G. 2011. Cooking enhances the antioxidant properties of some tropical green leafy vegetables. Afr J Biotechnol 10:632-639
  2. Ajandouz EH, Desseaux V, Tazi S, Puigserver A. 2008. Effects of temperature and pH on the kinetics of caramelisation, protein cross-linking and Maillard reactions in aqueous model systems. Food Chem 107:1244-1252 https://doi.org/10.1016/j.foodchem.2007.09.062
  3. Ajandouz EH, Tchiakpe LS, Ore FD, Benajiba A, Puigserver A. 2001. Effects of pH on caramelization and Maillard reaction kinetics in fructose-lysine model systems. J Food Sci 66:926-931 https://doi.org/10.1111/j.1365-2621.2001.tb08213.x
  4. Choi Y, Lee SM, Chun J, Lee HB, Lee J. 2006. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem 99:381-387 https://doi.org/10.1016/j.foodchem.2005.08.004
  5. Cotelle N, Bemier JL, Catteau JP, Pommery J, Wallet JC, Gaydou EM. 1996. Antioxidant properties of hydroxylflavones. Free Radic Biol Med 20:35-43 https://doi.org/10.1016/0891-5849(95)02014-4
  6. Dewanto V, Wu X, Adom KK, Liu RH. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50:3010-3014 https://doi.org/10.1021/jf0115589
  7. Du G, Li M, Ma F, Liang D. 2009. Antioxidant capacity and the relationship with polyphenol and vitamin C in Actinidia fruits. Food Chem 113:557-562 https://doi.org/10.1016/j.foodchem.2008.08.025
  8. Gill SS, Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plant. Plant Physiol Biochem 48:909-930 https://doi.org/10.1016/j.plaphy.2010.08.016
  9. Habib NC, Honore SM, Genta SB, Sanchez SS. 2011. Hypolipidemic effect of Smallanthus sonchifolius (yacon) roots on diabetic rats: Biochemical approach. Chem-Biol Interact 194:31-39 https://doi.org/10.1016/j.cbi.2011.08.009
  10. Hwang IG, Kim HY, Joung EM, Woo KS, Jeong JH, Yu KW, Lee J, Jeong HS. 2010. Changes in ginsenosides and antioxidant activity of Korean ginseng (Panax ginseng C.A. Meyer) with heating temperature and pressure. Food Sci Biotechnol 19:941-949 https://doi.org/10.1007/s10068-010-0132-9
  11. Hwang IG, Kim HY, Woo KS, Lee S, Jeong HS. 2011. Biological activities of Maillard reaction products (MRPs) in a sugar-amino acid model system. Food Chem 126:221-227 https://doi.org/10.1016/j.foodchem.2010.10.103
  12. Jeong SM, Kim SY, Kim DR, Jo SC, Nam KC, Ahn DU, Lee SC. 2004. Effect of heat treatment on the antioxidant activity of extracts from citrus peels. J Agric Food Chem 52:3389-3393 https://doi.org/10.1021/jf049899k
  13. Jia Z, Tang M, Wu J. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  14. Ju HK, Chung HW, Hong SS, Park JH, Lee J, Kwon SW. 2010. Effect of steam treatment on soluble phenolic content and antioxidant activity of the Chaga mushroom (Inonotus obliquus). Food Chem 119:619-625 https://doi.org/10.1016/j.foodchem.2009.07.006
  15. Kim AR, Lee JJ, Jung HO, Lee MY. 2010a. Physicochemical composition and antioxidative effects of yacon (Polymnia sonchifolia). J Life Science 20:40-48 https://doi.org/10.5352/JLS.2010.20.1.040
  16. Kim AR, Lee JJ, Lee YM, Jung HO, Lee MY. 2010b. Cholesterollowering and anti-obesity effects of Polymnia sonchifolia Poepp. & Endl. powder in rats fed a high fat-high cholesterol diet. J Korean Soc Food Sci Nutr 39:210-218 https://doi.org/10.3746/jkfn.2010.39.2.210
  17. Lan X, Liu P, Xia S, Jia C, Mukunzi D, Zhang X, Xia W, Tian H, Xiao Z. 2010. Temperature effect on the non-volatile compounds of Maillard reaction products derived from xylosesoybean peptide system: Further insights into thermal degradation and cross-linking. Food Chem 120:967-972 https://doi.org/10.1016/j.foodchem.2009.11.033
  18. Manzocco L, Calligaris S, Mastrocola D, Nicoli MC, Lerici CR. 2001. Review of non-enzymatic browning and antioxidant capacity in processed foods. Trends Food Sci Technol 11: 340-346
  19. Martins SIFS, Van Boekel MAJS. 2005. A kinetic model for the glucose/glycine Maillard reaction pathways. Food Chem 90:257-269 https://doi.org/10.1016/j.foodchem.2004.04.006
  20. Niki E. 2010. Assessment of antioxidant capacity in vitro and in vivo. Free Radic Biol Med 49:503-515 https://doi.org/10.1016/j.freeradbiomed.2010.04.016
  21. Ojansivu I, Ferreira CL, Salmine S. 2011. Yacon, a new source of prebiotic oligosaccharides with a history of safe use. Trends Food Sci Technol 22:40-46 https://doi.org/10.1016/j.tifs.2010.11.005
  22. Park JS, Yang JS, Hwang BY, Yoo BK, Han K. 2009. Hypoglycemic effect of yacon tuber extract and its constituent, chlorogenic acid, in streptozotocin-induced diabetic rats. Biomol Ther 17:256-262 https://doi.org/10.4062/biomolther.2009.17.3.256
  23. Ratnam DV, Ankola DD, Bhardwaj V, Sahana DK, Kumar MNVR. 2006. Role of antioxidants in prophylaxis and therapy: A pharmaceutical perspective. J Control Release 113:189-207 https://doi.org/10.1016/j.jconrel.2006.04.015
  24. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  25. Takenaka M, Yan X, Ono H, Yoshida M, Nagata T, Nakanishi T. 2003. Caffeic acid derivatives in the roots of yacon (Smallanthus sonchifolius). J Agric Food Chem 51:793-796 https://doi.org/10.1021/jf020735i
  26. Tepe B, Sokmen M, Akpulat HA, Sokmen A. 2006. Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chem 95: 200-204 https://doi.org/10.1016/j.foodchem.2004.12.031
  27. Wang S, Melnyk JP, Tsao R, Marcone MF. 2011. How natural dietary antioxidants in fruits, vegetables and legumes promote vascular health. Food Res Int 44:14-22 https://doi.org/10.1016/j.foodres.2010.09.028
  28. Woo KS, Jang KI, Kim KY, Lee HB, Jeong HS. 2006. Antioxidative activity of heat treated licorice (Glycyrrhiza uralensis Fisch) extracts. Korean J Food Sci Technol 38:355-360
  29. Wootton-Beard PC, Ryan L. 2011. Improving public health?: The role of antioxidant-rich fruit and vegetable beverages. Food Res Int 44:3135-3148 https://doi.org/10.1016/j.foodres.2011.09.015
  30. Yan X, Suzuki M, Ohnishi-Kameyama M, Sada Y, Nakanishi T, Nagata T. 1999. Extraction and identification of antioxidants in roots of yacon (Smallanthus sonchifolius). J Agric Food Chem 47:4711-4713 https://doi.org/10.1021/jf981305o
  31. Yu X, Zhao M, Hu J, Zeng S, Bai X. 2012. Correspondence analysis of antioxidant activity and UV-Vis absorbance of Maillard reaction products as related to reactants. LWTFood Sci Technol 46:1-9 https://doi.org/10.1021/es204242a

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