Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Microbial Diversity of the Trichloroethylene Contaminated Groundwater Treatment System and Characterization of Pseudomonas sp. DHC8

Trichloroethylene으로 오염된 지하수 제거공정의 미생물 다양성 및 분리균주 Pseudomonas sp. DHC8의 특성

  • Nam, Ji-Hyun (Department of Biology, Kyungsung University) ;
  • Shin, Ji-Hye (Department of Microbiology, Chungbuk National University) ;
  • Kwon, Kiwook (Department of Civil and Environmental System Engineering, Hanyang University) ;
  • Bae, Wookeun (Department of Civil and Environmental System Engineering, Hanyang University) ;
  • Lee, Dong-Hun (Department of Microbiology, Chungbuk National University)
  • 남지현 (경성대학교 생물학과) ;
  • 신지혜 (충북대학교 미생물학과) ;
  • 권기욱 (한양대학교 건설환경플랜트공학과) ;
  • 배우근 (한양대학교 건설환경플랜트공학과) ;
  • 이동훈 (충북대학교 미생물학과)
  • Received : 2013.09.23
  • Accepted : 2013.10.21
  • Published : 2013.12.31
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Abstract

Trichloroethylene (TCE) is a widely used substance in commercial and industrial applications, yet it must be removed from the contaminated soil and groundwater environment due to its toxic and carcinogenic nature. We investigated bacterial community structure, dominant bacterial strain, and removal efficiency in a TCE contaminated groundwater treatment system using immobilized carrier. The microbial diversity was determined by the nucleotide sequences of 16S rRNA gene library. The major bacterial population of the contaminated groundwater treatment system was belonging to BTEX degradation bacteria. The bacterial community consisted mainly of one genus of Pseudomonas (Pseudomonas putida group). The domination of Pseudomonas putida group may be caused by high concentration of toluene and TCE. Furthermore, we isolated a toluene and TCE degrading bacterium, named Pseudomonas sp. DHC8, from the immobilized carrier in bioreactor which was designed to remove TCE from the contaminated ground water. Based on the results of morphological and physiological characteristics, and 16S rRNA gene sequence analysis, strain DHC8 was identified as a member of Pseudomonas putida group. When TCE (0.83 mg/L) and toluene (60.61 mg/L) were degraded by this strain, removal efficiencies were 72.3% and 100% for 12.5 h, respectively. Toluene removal rate was 2.89 ${\mu}mol/g$-DCW/h and TCE removal rate was 0.02 ${\mu}mol/g$-DCW/h. These findings will be helpful for maintaining maximum TCE removal efficiency of a reactor for bioremediation of TCE.

산업에서 널리 사용되고 있는 Trichloroethylene (TCE)은 토양 및 지하수의 오염을 일으키며, 암 유발물질로 환경에서 반드시 제거해야 하는 물질이다. 본 연구에서는 미생물 고정화 담체를 이용한 TCE로 오염된 지하수 처리 시스템의 세균 군집구조를 조사하고, 우점종을 분리 및 동정하고 TCE 제거특성을 확인하였다. TCE로 오염된 지하수 처리공정의 세균군집을 16S rRNA 유전자 라이브러리의 염기서열 분석방법을 이용하여 조사한 결과, 주요 개체군은 BTEX 분해세균으로 알려진 Pseudomonas 속이었으며 Pseudomonas putida 그룹이 가장 우점하였다. Pseudomonas putida 그룹의 우점은 높은 toluene과 TCE의 농도에서 기인한 것으로 생각된다. TCE로 오염을 제거하기 위한 미생물 반응기에서 toluene과 TCE 분해 세균을 분리 배양하였으며 Pseudomonas sp. DHC8로 명명하였다. 형태학적 특징, 생리 생화학적 특징, 16S rRNA 유전자 염기서열분석 결과 DHC8 균주는 P. putida 그룹에 속하는 것으로 확인되었다. Pseudomonas sp. DHC8을 이용하여 TCE (0.83 mg/L)와 toluene (60.61 mg/L)에 대해 분해실험을 실시하였을 때 12.5시간 동안 TCE는 72.3%, toluene은 100.0% 제거되었다. 또한, TCE와 toluene의 제거속도는 각각 0.02 ${\mu}mol/g$-DCW/h와 2.89 ${\mu}mol/g$-DCW/h였다. 본 연구 결과는 TCE의 생물정화를 위한 반응기의 최대 효율을 유지하기 위한 노력에 도움이 될 것이다.

Keywords

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