Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Biocontrol of Red Pepper Using Mixed Culture of Antagonistic Bacterium and Phosphate Solubilizing Yeast

항진균 세균과 난용성 인산염 가용화 효모의 혼합 배양액을 이용한 고추 병해의 생물학적 방제

  • Lee, Gun Woong (Division of Biotechnology and Advanced Institute of Environmental and Bioscience, Chonbuk National University) ;
  • Min, Byung-Dae (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Park, Sujeong (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Jheong, Weonhwa (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Go, Eun Byeul (Division of Biotechnology and Advanced Institute of Environmental and Bioscience, Chonbuk National University) ;
  • Lee, Kui-Jae (Division of Biotechnology and Advanced Institute of Environmental and Bioscience, Chonbuk National University) ;
  • Chae, Jong-Chan (Division of Biotechnology and Advanced Institute of Environmental and Bioscience, Chonbuk National University)
  • 이건웅 (전북대학교 생명공학부 및 환경생명신기술연구소) ;
  • 민병대 (국립환경과학원 상하수도연구과) ;
  • 박수정 (국립환경과학원 상하수도연구과) ;
  • 정원화 (국립환경과학원 상하수도연구과) ;
  • 고은별 (전북대학교 생명공학부 및 환경생명신기술연구소) ;
  • 이귀재 (전북대학교 생명공학부 및 환경생명신기술연구소) ;
  • 채종찬 (전북대학교 생명공학부 및 환경생명신기술연구소)
  • Received : 2013.11.01
  • Accepted : 2013.11.19
  • Published : 2013.12.31
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Abstract

This study was to investigate beneficial effects of microbial mixture on red pepper which was capable of promoting plant growth by solubilizing insoluble phosphate as well as protecting plants from pathogenic attack. Saccharomyces sp. L13 was isolated for phosphate solubilizing activity on aluminium phosphate, tricalcium phosphate, calcium hydrophosphate, and magnesium hydrophosphate. On the other hand, Bacillus sp. L32 was isolated for antagonistic activity against Phytophthora capsisi and Colletotrichum gloeosporioides, causing Phytophthora blight and Anthracnose disease in pepper, respectively. The strain L32 exhibited antagonistic activities both under dual culture assays and detached leaves assays. The each strain under the condition of mixed cultivation exhibited the same growth rates as one under pure cultivation. In greenhouse study, the mixed culture showed the both effect of plant growth promotion and reduction of disease symptom development against P. capsisi and C. gloeosporioides providing a potential as effective microbial agent for plant husbandry.

고추생장 촉진을 위해 병원균에 대한 방제력과 난용성 인산염에 대한 가용화 활성을 보이는 미생물 혼합배양액의 효과를 검증하였다. Saccharomyces sp. L13은 난용성 인산염에 대한 가용화 활성으로 분리되었으며 Bacillus sp. L32는 고추역병과 고추탄저병에 대한 길항력 활성으로 분리되었다. 특히 L32 균주는 대치배양법과 잎을 이용한 실증실험에서 모두 길항능력을 보였으며 시설재배를 이용한 실증실험에서도 병의 발병율을 저감시켰다. 두 균주의 혼합배양은 각 균주들의 생장율에 영향을 주지 않았으며 고추에 대한 혼합배양액 처리는 고추역병과 고추탄저병의 발병율을 저감시키는 동시에 난용성 인산염의 가용화를 통해 고추의 생장율을 증대시키는 효과를 확인할 수 있었다. 본 결과는 두 균주의 혼합배양액이 작물재배를 위한 미생물제제로서 잠재적 효용성이 높다는 것을 제시한다.

Keywords

References

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