The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Neuroprotective effects of some herbal medicine plant extract against ischemia·reperfusion-induced cell death in SK-N-SH neuronal cells

허혈·재관류 유도성 신경세포사멸에 대하여 신경보호효과를 가지는 약용식물 추출물의 검색

  • Oh, Tae-Woo (Department of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Lee, Mi Young (AAing Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Hye Won (AAing Research Center, Korea Institute of Oriental Medicine) ;
  • Park, Yong-Ki (Department of Herbology, College of Oriental Medicine, Dongguk University)
  • 오태우 (동국대학교 한의과대학 본초학교실) ;
  • 이미영 (한국한의학연구원) ;
  • 이혜원 (한국한의학연구원) ;
  • 박용기 (동국대학교 한의과대학 본초학교실)
  • Received : 2013.02.20
  • Accepted : 2013.03.21
  • Published : 2013.03.30
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Abstract

Objectives : The purpose of the study is to determine the neuroprotective effects of the water and 80% EtOH extract of some herbal medicine plant on ischemia reperfusion-induced cell death in SK-N-SH human brain neuronal cells. Methods : SK-N-SH cells were treated with 3mM sodium azide and 10 mM 2-deoxy-D-glucose for 45 min, ptior to the addition of different concentrations of herbal medicine plant extract (0, 10, 25, 50, 100, 250, 500, 1000 ${\mu}g/ml$) for 2 hr and then reperfused with growth medium, incubated for 24 h. Cell viability was determined by WST-1 assay, and ATP/ADP levels were measured by ADP/ATP ratio assay kit. Results : Herbal medicine plant extract significantly inhibited decreasing the cell viability in ischemia-induced SK-N-SH cells. Also increased the ratio of ADP/ATP in ischemia-induced neuronal cells. Conclusions : Our results suggest that herbal medicine plant extract has a neuroprotective property via increasing the energy levels in neuronal cells, suggesting that extract may has a therapeutic potential in the treatment of ischemic brain injury. The exact component and mechanism remains for the future study.

Keywords

References

  1. Kalaria RN, Ballard. Overlap between pathology of Alzheimer disease and vascular dementia. Alzheimer Dis Assoc Disord. 1999 ; 13(suppl3) : S115-23
  2. Shah S, Tangalos EG, Petersen R. Mild cognitive impairment: When is it a precursor of Alzheimer’s disease?. Geriatrics. 2000 ; 55 : 65-8
  3. Omodeo-Sale F, Granigna D, Camoaniello R. Lipid peroxidation and antioxidant systerms in rat brain: effect of chronic alcohol consumption. Neurochem. 1997 ; 22 : 557-82
  4. Good PF, Werner P, Hsu A, Olanow CW, Perl DP. Evidence for neuronal oxidative damage in Alzheimer’s disease. Am J Pathol. 1996 ; 149 : 21-8
  5. Choi WH, Oh YS, Ahn JY, Kim SR, Ha TY. Antioxidative and protective effects of Ulmus davidiana var. japonica extracts on glutammate-induced cytotoxicity in PC12 cells. Korean J Food Sci Technol. 2005 ; 37 : 479-83.
  6. Kurogi M, Bessho Y. Shokuhin Sogo Kenkyujo. Kenkyu Hokoku (Report of National Food Research Institute). 1980 ; 36 : 33-7.
  7. Choi OB, Kim KM, Yoo GS, Park KH. Anti-allergic effect of Castanea crenata leaf tea. Korean J Food Sci Technol. 1998 ; 30: 468-71.
  8. Kwon EJ, Kim YC, Kwon MS, Kim CS, Kang WW, Lee JB, Chung SK.Antioxidative activity of solvent fraction and isolation of antioxidant compound from chestnut husk. J Korean Soc Food Sci Nutr. 2001 ; 30 : 726-31.
  9. Nha YA, Yang CB. Change of constituent components in chestnut during storage. Korean J Food Sci Technol 1996 ; 28 : 1164-70.
  10. Kim SK, Jeon YJ, Kim YT, Lee BJ, Kang OJ. Sensory evaluation and retrogradation properties of chestnut mook. J Korean Soc Food Nutr. 1995 ; 24 : 601-5.
  11. Cho SH, Sung NK, Ki WK, Hur JH, Shim KH, Chung DH. Effect of blanching on the prevention of discoloration in the thermal-treated chestnut power. J Korean Soc Food Nutr. 1988 ; 17 : 211-4.
  12. Lee SI, Ahn DG, Shin MK, Lee YG. Oriental medicine clinical applications. Seoul : Seongbosa. 1986 : 182-3, 357-8.
  13. Lee CB. Forest economics-mokchoganagmok. Korean plant map. Seoul : Hyangminsa. 1982 : 29
  14. Chung TY, Cho DS, Song JC. Nonvolatile/volatile flavor components in chinese qince fruits, Chanenomeles sinensis Koehne. Korea J Food Sci Technol. 1988 ; 20 : 293-302
  15. Song JC, Cho EK, Park HJ. Studies of manufacture of mixed beverage drinks using chinese quince and apple. Food Eng Prog. 2002 ; 6 : 38-45
  16. Lee DH, Kim JH, Kim NM, Choi JS, Lee JS. Physiological functionality of chinese quince wine and liqors. Korean J Biiotechnol Bioeng. 2002 ; 17 : 266-70
  17. Kim YS, Lee SW, Lee KR, Kim SK, Cho SY, Lee JH. Studies on tasty constituents in various foodstuffs. Part 1. Tasty constituents of chinese quince. Korea J Food Sci Technol. 1971 ; 3 : 163-7
  18. An DG. Korean herbal illustrated book. Seoul : 2003 : 307-50
  19. National Chinese Medicine Bureau. Chinese herbal. Shanghai. Shanghai Science and Technology Publisher : 1999.
  20. Shih CK, Son YJ, Lee YJ. Changes in the carbohydrate contents of Rehmanniae Radix during processing. Kor J Herbology. 1999 ; 14 : 1-11.
  21. Hong SP, Kim YC, Kim KH, Park JH, Park MK. Characteristic component of Rehmanniae radix Preparata compared to Rehmanniae radix and Rehmanniae radix Crudus. J Korean Soc Anal Sci. 1993 ; 6 : 401-4.
  22. Park SJ, Park HS, Yoo SO. Effects of supplemention of Rehmannia radix on performance and physiological status in broiler chicks. Korean J Poult Sci. 1998 ; 25 : 195-202.
  23. Chen LZ, Feng XW, Zhou JH. Effects of Rehmannia glutinosa polysaccharide b on T-lymphocytes in mice bearing sarcoma 180. Zhongguo Yao Li Xue Bao. 1995 ; 16 : 337-40
  24. Tomoda M, Miyamoto H, Shimizu N, Gonoda R, Ohara N. Two acidic polysaccharides having reticuloendothelial system-potentiating activity from the raw root of Rehmannia glutinosa. Biol Pharm Bull. 1994 ; 17 : 1456-9. https://doi.org/10.1248/bpb.17.1456
  25. Tomoda M, Miyamoto H, Shimizu N. Structural features and anti-complementary activity of rehmannan SA, a polysaccharide from the root of Rehmannia glutinosa. Chem Pharm Bull (Tokyo). 1994 ; 42 : 1666-8. https://doi.org/10.1248/cpb.42.1666
  26. Herbology department of all-korea oriental medicine collge. Herbalogy. Seoul. Yongrimsa : 2004 : 647-9
  27. Park SD, Lee GH, Lee YS, Kwun YK, Park JH, Choi SM, Shin SW. Comparison of Immunomodualtory Effects of Water-extracted Adenophorae Radix, Liriopis Tuber, Dendrobii Herba, Polygonati Odorati Rhizoma and Polygonati Rhizoma. Kor J Ori Med Physiol Pathol. 2007 ; 21(2) : 414-24.
  28. Min HB, Roh SS, Seo HS. Effects of Dendrobii herba and Punica granatum Extract on the Anti-oxidant, Anti-inflammatory, Anti-wrinkle and Whitening. J Korean Orient Med Ophthalmol Otolaryngol Dermato. 2010 ; 22(3) : 11-32
  29. Yoon MY, Kim JY, Hwang JH, Cha MR, Lee MR, Jo KJ, Park HR. Protective Effect of Methanolic Extracts from Dendrobium nobile Lindl. on H2O2-induced Neurotoxicity in PC12 cells. J Korean Soc Appl Biol Chem. 2007 ; 50(1) : 63-7.
  30. Oh TW, Park KH, Lee MY, Choi GY, Park YK. Effects of the water extract from Achyranthis Radix on serum-deprivation-induced apoptosis in PC12 cells and transient cerebral middle artery occlusion-induced ischemic brains of rats. Kor J Herbology. 2012；27(2)：77-83. https://doi.org/10.6116/kjh.2012.27.2.77
  31. Tabakman R, Jiang H, Shahar I, Arien-Zakay H, Levine RA, Lazarovici P. Neuroprotection by NGF in the PC12 in vitro OGD model: involvement of mitogen-activated protein kinases and gene expression. Ann N Y Acad Sci. 2005 ; 1053 : 84-96 https://doi.org/10.1196/annals.1344.008
  32. Cohadon F, Rigoulet M, Averet N. Alterations of membrane-bound enzymes in vasogenic edema. In: Go KG, Baethmann A (eds) Recent progress in the study and therapy of brain edema. New York Plenum : 1983 : 223-32.
  33. Lee HG, Kim YS. Effects of Salvia Miltriorrhiza Radix on Neuronal Apoptosis following Intracerebral Hemorrhage of Rats. Kor J Herbology. 2012 ; 27(3)：89-94. https://doi.org/10.6116/kjh.2012.27.3.89
  34. Kirno T, Tamura A, Aano K. Delayed neuroral death in the rat hipoccampus following transient forebrain ischemia. Acta Neuropathol(Beri). 1984 ; 64 : 139-47. https://doi.org/10.1007/BF00695577
  35. Torii K, Sugiyama S, Takagi K, Satake T and Ozawa T. Age-related decrease in respiratory muscle mitochondrial function in rats. Am J Resp Cell Mol Biol. 1992 ; 6 : 88-92. https://doi.org/10.1165/ajrcmb/6.1.88
  36. Heo J. Dongeuibogam. Seoul. Bubin publishers : 2005 : 1886.
  37. Noh JR. Antioxidant effects of the chestnut (Castaneaecrenata) inner shell extract in t-BHP-treated HepG2 cells, and CCl4 and high-fatdiet-treated mice. Fddf Chenm Toxicol. 2010 ; 48 : 3177-83. https://doi.org/10.1016/j.fct.2010.08.018
  38. Lee SW. The effect of Extract from Taeumjowe-tang and Coicis Semen Castaneae Semen on Palmitic-acid induced lipotoxicity in HepG2 cell. Kyung Hee University. 2011.
  39. Lee JD. The effects of dendrobii herba against intracerebral hemorrhage in rat. Kyung Won University. 2011.
  40. Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. Biol Pharmaceut Bull. 1996 ; 19 : 1518-20. https://doi.org/10.1248/bpb.19.1518