Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Antioxidant Activity and Its Mechanism of Chelidonium majus Extract

백굴채 추출물의 항산화 활성과 기전

  • Heo, Jee In (Department of Biochemistry, Hallym University Medical School) ;
  • Kim, Jeong Hyeon (Department of Biochemistry, Hallym University Medical School) ;
  • Lee, Jeong Min (Department of Biochemistry, Hallym University Medical School) ;
  • Lim, Soon Sung (Institute of Natural Medicine, Hallym University Medical School) ;
  • Kim, Sung Chan (Department of Biochemistry, Hallym University Medical School) ;
  • Park, Jae Bong (Department of Biochemistry, Hallym University Medical School) ;
  • Kim, Jae Bong (Department of Biochemistry, Hallym University Medical School) ;
  • Lee, Jae Yong (Department of Biochemistry, Hallym University Medical School)
  • 허지인 (강원도 춘천시 한림대학교 의과대학 생화학교실) ;
  • 김정현 (강원도 춘천시 한림대학교 의과대학 생화학교실) ;
  • 이정민 (강원도 춘천시 한림대학교 의과대학 생화학교실) ;
  • 임순성 (강원도 춘천시 한림대학교 의과대학 천연의약연구소) ;
  • 김성찬 (강원도 춘천시 한림대학교 의과대학 생화학교실) ;
  • 박재봉 (강원도 춘천시 한림대학교 의과대학 생화학교실) ;
  • 김재봉 (강원도 춘천시 한림대학교 의과대학 생화학교실) ;
  • 이재용 (강원도 춘천시 한림대학교 의과대학 생화학교실)
  • Received : 2013.01.28
  • Accepted : 2013.04.08
  • Published : 2013.04.30
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Abstract

Chelidonium majus (CM) contains several isoquinoline alkaloids that have been reported to have various biological activities such as anti-inflammatory, antimicrobial, antioxidant, immune-modulatory, and antitumoral. It has been reported that the extract of CM had an antioxidant potential, however the mechanism has not been verified. In this study, we found that CM extract activated FOXO3a. FOXO3a is a transcription factor that involved in various biological processes such as cell cycle arrest, apoptosis, DNA repair, and ROS detoxification. Transcriptional activities of FOXO3a were regulated by post-translational modifications including phosphorylation, acetylation, and ubiquitination. Protein level of FOXO3a was increased by CM extract. Promoter activities of FOXO-transcriptional target genes such as MnSOD, p27 and GADD45 were activated by CM extract in a dose dependent manner. In addition, protein level of MnSOD, major antioxidant enzyme, was increased by CM extract. Thereby ROS level was decreased by CM in old HEF cells. These results suggest that CM extract has an antioxidant activity through FOXO activation.

Keywords

References

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