Journal of Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
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Effects of Micro-Electrical Stimulation on Regulation of Behavior of Electro-Active Stem Cells

  • Im, Ae-Lee (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Kim, Jangho (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Lim, KiTaek (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Seonwoo, Hoon (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Cho, Woojae (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Choung, Pill-Hoon (Department of Oral and Maxillofacial Surgery and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Chung, Jong Hoon (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University)
  • Received : 2013.04.11
  • Accepted : 2013.05.24
  • Published : 2013.06.01
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Abstract

Purpose: Stem cells provide new opportunities in the regenerative medicine for human or animal tissue regeneration. In this study, we report an efficient method for the modulating behaviors of electro-active stem cells by micro-electric current stimulation (mES) without using chemical agents, such as serum or induction chemicals. Methods: Dental pulp stem cells (DPSCs) were cultured on the tissue culture dish in the mES system. To find a suitable mES condition to promote the DPSC functions, the response surface analysis was used. Results: We found that a working micro-current of 38 ${\mu}A$ showed higher DPSC proliferation compared with other working conditions. The mES altered the expressions of intracellular and extracellular proteins compared to those in unstimulated cells. The mES with 38 ${\mu}A$ significantly increased osteogenesis of DPSCs compared with ones without mES. Conclusions: Our findings indicate that mES may induce DPSC proliferation and differentiation, resulting in applying to DPSCs-based human or animal tissue regeneration.

Keywords

References

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