Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Administration Method on Blood Garcinia cambogia Concentration and Antioxidative Activity

투여 방법에 따른 가르시니아 캄보지아 혈중 농도 변화와 항산화 효과 변화

  • Park, Eun Jung (Dept. of Family Medicine, Cheil General Hospital, College of Medicine Kwandong University) ;
  • Kim, Sang Ho (Dept. of Family Medicine, Cheil General Hospital, College of Medicine Kwandong University) ;
  • Kim, Kyung Soo (Dept. of Family Medicine, Seoul Saint Mary's Hospital, The Catholic University) ;
  • Oh, Han Jin (Dept. of Family Medicine, Cheil General Hospital, College of Medicine Kwandong University)
  • 박은정 (관동의대 제일병원 가정의학과) ;
  • 김상호 (관동의대 제일병원 가정의학과) ;
  • 김경수 (가톨릭의대 서울성모병원 가정의학과) ;
  • 오한진 (관동의대 제일병원 가정의학과)
  • Received : 2013.02.07
  • Accepted : 2013.02.20
  • Published : 2013.06.30
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Abstract

The objective of this study was to investigate the effects of administration methods for on Garcinia cambogia on blood Garcinia cambogia concentration and antioxidative levels. Rats were divided into three groups: G1 (normal group), G2 (one administration of Garcinia cambogia extract 2,800 mg/kg b.w.), G3 (three separate administrations every 6 h of Garcinia cambogia extract 750, 850, and 1,200 mg/kg b.w. for 18 h). Blood samples were collected every hour, and animals sacrificed 18 h after the oral administration of Garcinia cambogia extract. We examined changes in the serum concentration of Garcinia cambogia by HPLC analysis. Two hours following an oral administration of Garcinia cambogia extract (2,800 mg/kg b.w.), serum Garcinia cambogia levels reached their maximum, but gradually decreased until 10 hours when it was no longer detectable. In contrast, serum Garcinia cambogia levels under G3 administration were maintained above a certain level after 18 h. To determine whether this level of Garcinia cambogia could affect blood oxidative levels, we measured serum lipid peroxidation by TBARS levels. TBARS levels from G3 treatment were significantly lower than G1 and G2. To analyze other antioxidative activities, radical scavenging activities were measured by the DPPH and ABTS radical scavenging assays. There were no significant differences between the groups in DPPH radical scavenging activity. However, ABTS radical scavenging activity significantly increased with G3 treatment compared with G1 and G2. In conclusion, our data show that three times administration of Garcinia cambogia every 6 h may helpful for maintaining serum Garcinia cambogia levels and antioxidative effects.

본 실험에서는 $Ca^{2+}/K^+$가 결합된 가르시니아 캄보지아 추출물(HCA 63%)을 이용하여 투여방법에 따른 혈중 가르시니아 캄보지아의 농도 변화와 이에 따른 혈중 항산화 효과의 변화를 살펴보았다. 정상군(G1), 가르시니아 캄보지아 추출물 2,800 mg/kg b.w.를 단회 투여한 군(G2)과 6시간 간격으로 750, 850, 1,200 mg/kg b.w의 농도로 높여가면서 3회 투여한 군(G3)으로 분리를 하여 실험을 진행하였다. 그 결과 단회 고농도 투여한 경우에는 10시간 이후에 혈중에 가르시니아 캄보지아가 분석되지 않은 반면, 3회로 나누어 투여한 경우에는 18시간 후에도 일정 농도 이상으로 지속되었음을 확인하였다. 또한 6시간 간격으로 3회 투여한 그룹의 동물에서 과산화지질 억제 효과를 보였으며, DPPH 라디칼 소거능에서 변화는 없었으나 ABTS 라디칼 소거능에서 유의적으로 높은 활성을 보였다. 따라서 가르시니아 캄보지아 추출물을 단회로 고용량 투여한 경우보다 3회로 저용량 투여한 경우에 혈중 가르시니아 캄보지아를 일정 농도 이상으로 유지 시켰으며 부분적으로 항산화 효과가 더 높게 나타났다.

Keywords

References

  1. Sullivan AC, Triscari J, Spiegel JE. 1977. Metabolic regulation as a control for lipid disorders. II. Influence of (-)-hydroxycitrate on genetically and experimentally induced hypertriglyceridemia in the rat. Am J Clin Nutr 30:777-784.
  2. Ohia SE, Opere CA, LeDay AM, Bagchi M, Bagchi D, Stohs SJ. 2002. Safety and mechanism of appetite suppression by a novel hydroxycitric acid extract (HCA-SX). Mol Cell Biochem 238: 89-103. https://doi.org/10.1023/A:1019911205672
  3. Kolodziejczyk J, Masullo M, Olas B, Piacente S, Wachowicz B. 2009. Effects of garcinol and guttiferone K isolated from Garcinia cambogia on oxidative/nitrative modifications in blood platelets and plasma. Platelets 20: 487-492. https://doi.org/10.3109/09537100903165182
  4. Amin KA, Kamel HH, Abd Eltawab MA. 2011. Protective effect of Garcinia against renal oxidative stress and biomarkers induced by high fat and sucrose diet. Lipids Health Dis 13: 6.
  5. Jena BS, Jayaprakasha GK, Singh RP, Sakariah KK. 2002. Chemistry and biochemistry of (-)-hydroxycitric acid from Garcinia. J Agric Food Chem 50: 10-22. https://doi.org/10.1021/jf010753k
  6. Sullivan AC, Singh M, Srere PA, Glusker JP. 1977. Reactivity and inhibitor potential of hydroxycitrate isomers with citrate synthase, citrate lyase, and ATP citrate lyase. J Biol Chem 252: 7583-7590.
  7. Sterling GH, McCafferty MR, O'Neill JJ. 1981. ${\beta}$-Hydroxybutyrate as a precursor to the acetyl moiety of acetylcholine. J Neurochem 37: 1250-1259. https://doi.org/10.1111/j.1471-4159.1981.tb04675.x
  8. Ricny J, Tucek S. 1982. Acetylcoenzyme A and acetylcholine in slices of rat caudate nuclei incubated with (-)-hydroxycitrate, citrate, and EGTA. J Neurochem 39: 668-673. https://doi.org/10.1111/j.1471-4159.1982.tb07944.x
  9. Downs BW, Bagchi M, Subbaraju GV, Shara MA, Preuss HG, Bagchi D. 2005. Bioefficacy of a novel calcium-potassium salt of (-)-hydroxycitric acid. Mutat Res 579: 149-162. https://doi.org/10.1016/j.mrfmmm.2005.02.021
  10. Halliwell B, Gutteridge JM. 1984. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219: 1-14.
  11. Gutiérrez RM, Mitchell S, Solis RV. 2008. Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 117: 1-27. https://doi.org/10.1016/j.jep.2008.01.025
  12. Masteikova R, Muselik J, Bernatoniene J, Majiene D, Savickas A, Malinauskas F, Bernatoniene R, Peciura R, Chalupova Z, Dvorackova K. 2008. Antioxidant activity of tinctures prepared from hawthorn fruits and motherwort herb. Ceska Slov Farm 57: 35-38.
  13. Fraga CG, Leibovitz BE, Tappel AL. 1988. Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes. Free Radic Biol Med 4: 155-161. https://doi.org/10.1016/0891-5849(88)90023-8
  14. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  15. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  16. Asberg M, Crönholm B, Sjöqvist F, Tuck D. 1971. Relationship between plasma level and therapeutic effect of nortriptyline. Br Med J 3: 331-334. https://doi.org/10.1136/bmj.3.5770.331
  17. Cho SH, Choi YJ, Rho CW, Choi CY, Kim DS, Cho SH. 2008. Reactive oxygen species and cytotoxicity of bamboo (Phyllostachys pubescens) sap. Korean J Food Preserv 15:105-110.
  18. Sanchez-Moreno C. 2002. Review: methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int 8: 121-137. https://doi.org/10.1177/1082013202008003770

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