Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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The Effects of Dietary Supplementation of Vitamin C or E on the Expressions of Endoplasmic Reticulum Stress, Lipid and Glucose Metabolism Associated Genes in Broiler Chickens

비타민 C 및 E의 첨가 급여가 육계의 소포체 스트레스와 지방 및 포도당 대사 연관 유전자의 발현에 미치는 영향

  • Park, Jeong Geun (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • An, Young Sook (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Sohn, Sea Hwan (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Jang, In Surk (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Moon, Yang Soo (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology)
  • 박정근 (경남과학기술대학교 동물생명과학과) ;
  • 안영숙 (경남과학기술대학교 동물생명과학과) ;
  • 손시환 (경남과학기술대학교 동물생명과학과) ;
  • 장인석 (경남과학기술대학교 동물생명과학과) ;
  • 문양수 (경남과학기술대학교 동물생명과학과)
  • Received : 2013.06.12
  • Accepted : 2013.06.13
  • Published : 2013.06.30
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Abstract

This study was conducted to evaluate the effects of dietary supplementation of vitamin C or E on the expressions of endoplasmic reticulum (ER) stress, lipid and glucose metabolism associated genes in broiler chickens. A total of 216 one-day-old male broilers was randomly alloted to 4 treatments with 6 replicate pens per treatment and 9 broilers per pen for 35 days. The dietary treatments were control, vitamin C (control diet + ascorbic acid 200 mg/kg diet), vitamin E (control diet + ${\alpha}$-tocopherol 100 mg/kg diet), vitamin C + E (control diet + vitamin C 200 mg/kg + vitamin E 100 mg/kg), respectively. To evaluate gene expressions by quantitative real-time polymerase chain reaction, total RNA was extracted from the liver of the chicken at 35 days of age. Dietary supplementation of vitamins was significantly down-regulated the expression of stress marker genes including HSP70, HSP90, and HMGCR, as compared to the control (p<0.05). The expressions of ER stress associated genes also inhibited by supplementation of vitamins as well (p<0.05). Vitamin C supplementation suppressed the expression of lipid associated genes such as FASN, FATP1 and ACSL1. Vitamin supplementation did not affect the glucose transporters, GLUT2 and GLUT8, in the liver. The results of the present study indicated that dietary supplementation of vitamin C or E could be beneficial for the alleviating physiological stress in broiler chickens.

본 연구는 육계에서 비타민 C와 E의 첨가 급여가 소포체(ER) 스트레스 및 지방 및 포도당대사 연관 유전자들의 발현에 미치는 영향을 살펴보고자 실시하였다. 육계에 비타민 첨가 급여 후 5주령에 닭의 간을 취하여 유전자들의 발현을 real-time PCR로 비교 분석하였다. 육계의 비타민 C 및 E 첨가 급여는 HSP70, HSP90 및 HMGCR 스트레스 마커 유전자들의 발현을 감소시켰다. ER 스트레스 관련 유전자들 또한 스트레스 마커 유전자들과 마찬가지로 비타민 처리에 의하여 대조구에 비하여 낮은 발현 양상을 보여줌으로서, 대표적 스트레스 마커 유전자들과 더불어 세포 내 ER stress도 영향을 받을 수 있음을 보여 주었다. 육계의 비타민 첨가 급여는 대조구에 비하여 지방대사 연관 유전자들의 발현이 비타민 첨가구에서 감소함에 따라 지방대사에도 영향을 미치고 있음을 보여주었다. 비타민의 첨가 유무와 관계없이 간세포 내부로 포도당을 운반하는 운반체인 GLUT 단백질들의 발현에는 큰 영향을 주지 못하였다. 본 연구의 결과는 육계에 사료 내 비타민 C 또는 E의 첨가급여가 닭의 스트레스 정도를 완화시킬 수 있으며, 또한 지방합성 대사에도 영향을 미칠 수 있음을 세포 수준의 관련 유전자들의 분석을 이용하여 검증할 수 있음을 보여 주었다.

Keywords

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