Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Characteristics of Fermented Dropwort Extract and Vinegar Using Fermented Dropwort Extract and Its Protective Effects on Oxidative Damage in Rat Glioma C6 Cells

미나리 발효액과 미나리 발효액을 이용한 식초의 특성 분석 및 glioma C6 세포에서 산화적 손상에 대한 보호 효과

  • 김민주 (계명대학교 식품가공학과) ;
  • 이삼빈 (계명대학교 식품가공학과) ;
  • 최준혁 (계명대학교 식품가공학과) ;
  • 권승혁 ((주)생생초) ;
  • 김형대 (비슬청록농장) ;
  • 방면호 (경희대학교 피부생명공학센터) ;
  • 양선아 (계명대학교 전통미생물자원개발 및 산업화연구센터)
  • Received : 2012.12.20
  • Accepted : 2013.04.01
  • Published : 2013.06.30
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Abstract

The quality of fermented dropwort extract (FDE) and fermented dropwort vinegar (FDV) was assessed for free sugar, organic acid and free and total amino acid content. Major organic acids were lactic acid in FDE and acetic acid in FDV. Free sugars in FDE were fructose and glucose, and those in FDV were fructose, sucrose, and maltose. Aspartic acid was the major free amino acid in both FDE and FDV. Additionally, the main free amino acids in FDE were alanine and ${\gamma}$-amino-n-butyric acid (GABA), while those in FDV were arginine and valine. Moreover, to investigate the protective effects of FDE and FDV against oxidative stress induced by t-BHP and $H_2O_2$, C6 cells were treated with FDE or FDV prior to inducing the oxidative damage. FDE and FDV inhibited cell death significantly in a dose-dependent manner. These data imply that FDE and FDV may be effective in neuronal cell protection against oxidative damage.

본 연구에서는 효모를 이용하여 제조한 미나리 천연 식초 및 원료로 사용된 미나리 발효액의 유리당, 유기산, 아미노산 함량을 측정하였으며, 미나리 식초의 뇌 기능 개선 관련 기능성을 확인하기 위하여 C6 흰쥐 신경아교세포를 이용하여 산화적 손상에 대한 보호 효과를 검토하였다. 미나리 식초는 약 5%의 초산을 함유하였으며 glucose는 검출되지 않아 초산발효가 잘 이루어진 것을 알 수 있다. 또한 유리 아미노산의 함량은 높지 않았으나 그 중 aspartic acid, arginine 그리고 valine이 주요 유리 아미노산으로 나타났으며, 발효액에서는 aspartic acid, alanine, GABA의 순으로 나타났다. 총 아미노산은 식초에서는 glutamic acid와 glutamine의 함량이 가장 높았으며, 발효액의 경우 aspartic acid와 asparagine이 주요 총 아미노산으로 나타났다. 미나리 식초의 효능을 평가하기 위하여 식초를 동결 건조하여 분말화한 식초의 산화적 손상에 대한 뇌 신경세포 보호 효과를 측정한 결과, 발효액과 식초 모두 C6 세포에 대한 독성은 없었으며 t-BHP 및 $H_2O_2$에 의한 세포 생존율 저하를 유의적으로 억제하는 효과를 확인하였다.

Keywords

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