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Fermentation and Quality Characteristics of Cheonggukjang with Addition of Dropwort (Oenanthe javanica D.C.) Powder

미나리 분말을 첨가한 청국장의 발효 및 품질특성

  • Lee, Shin-Ho (Dept. of Food Science and Technology, Catholic University of Daegu) ;
  • Kim, Jin-Hak (Dept. of Food Science and Technology, Catholic University of Daegu)
  • 이신호 (대구가톨릭대학교 식품가공학과) ;
  • 김진학 (대구가톨릭대학교 식품가공학과)
  • Received : 2013.03.12
  • Accepted : 2013.04.11
  • Published : 2013.07.31

Abstract

Characteristics of Cheonggukjang with addition of different dropwort (Oenanthe javanica D.C.) powders were investigated. The selected strain, with proteolytic, amylolytic, and antimicrobial activity, was identified as B. subtilis RS-9, using 16S rRNA analysis. The Cheonggukjang was prepared with cooked soybean without dropwort (Control), 0.5% raw dropwort powder (DW0.5), and 1% raw dropwort powder (DW1), 0.5% steamed dropwort powder (SDW0.5), and 1% steamed dropwort powder (SDW1) were added, respectively. The changes in pH of Cheonggukjangs with addition of dropwort powder were lower than those of control during fermentation for 72 hr at $40^{\circ}C$. The total aerobes of the various Cheonggukjangs reached 8.88 (control), 8.82 (DW0.5), 8.70 (DW1), 8.85 (SDW0.5), and 8.75 (SDW1) log CFU/mL after fermentation for 72 hr at $40^{\circ}C$, respectively. The amino nitrogen and viscous substance contents of different dropwort powders added to Cheonggukangs were lower than those of control. The total polyphenol contents and ABTS radical scavenging ability of various Cheonggukjangs were increased by addition of dropwort powder and fermentation. The polyphenol contents and ABTS radical scavenging ability of SDW1 were $590.24{\mu}g/mL$ and 82.16% and showed the highest value among tested Cheonggukangs. The sensory quality of DW0.5 was higher in taste and overall acceptability, compared with other groups.

미나리 유통과정중 생성되는 부산물의 활용방안과 청국장의 품질 및 기호성 개선 방안을 모색하기 위해 청국장의 제조시 전처리 방법을 달리한 미나리 분말 첨가에 따른 발효 및 품질 특성을 비교 검토하였다. 발효균주로 사용한 Bacillus subtilis RS-9은 볏짚으로부터 분리된 30 균주 중 대두 분해력, 단백 분해력, 전분 분해력, 항균활성이 가장 우수한 균주를 16S rRNA 염기서열 분석을 통해 동정한 후 사용하였다. 전처리 방법을 달리한 미나리 분말을 0.5%, 1%로 혼합하여 제조한 청국장의 발효 및 품질특성을 조사한 결과, 미나리 분말 혼합 청국장의 pH는 발효 전 기간 동안 대조구보다 낮은 경향을 나타내었으며, 첨가 미나리의 전처리 방법에 따라서는 뚜렷한 차이를 관찰할 수 없었다. 청국장의 발효 후 총균수는 8.70~8.88 log CFU/mL이었으며, 발효기간이 경과함에 따라 모든 처리구에서 증가하였다. 미나리 혼합 청국장의 아미노태 질소와 점질물 함량은 발효 전 기간 동안 대조구보다 낮은 경향을 나타내었다. 총 폴리페놀 함량은 발효후 모든 군이 증가하였으며, 스팀처리 미나리 첨가구가 $590.24{\mu}g/mL$로 가장 높은 함량을 나타내었다. ABTS radical 소거능은 대조구에 비해 뚜렷이 높았으며, 발효 후 모든 처리구에서 활성이 증가하였고 스팀처리 미나리 첨가구가 82.16%로 가장 우수하였다. 관능검사 결과는 생미나리 분말 0.5% 첨가군이 맛과 종합적 기호도에서 가장 우수하였다.

Keywords

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