Journal of the Korean Society of Visualization (한국가시화정보학회지)

The Korean Society of Visualization (한국가시화정보학회)
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Measurement of Diffusion Coefficient in Cell-Laden Agarose Gel with Different Cell Concentrations

아가로스 겔에 포함된 세포의 농도가 확산 계수에 미치는 영향 측정

  • 이병룡 (한국산업기술대학교 대학원 기계공학과) ;
  • 진송완 (한국산업기술대학교 기계공학과)
  • Received : 2013.02.28
  • Accepted : 2013.04.12
  • Published : 2013.04.25
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Abstract

In this study, diffusion coefficients of 20 kDa FITC-dextran in 2% agarose gel with different cell concentrations were measured using fiberoptic-based fluorescence recovery after photobleaching technique. As increasing cell concentration suspended in agarose gel, the diffusion coefficients were decreased. The diffusion coefficient of agarose gel which contains $10{\times}10^6$ cells/ml was decreased to 11% that of in agarose gel without cells. The distribution of fluorescence dye in 3D scaffold was also simulated. The simulation result shows that the diffusion coefficient is more significant factor than the scaffold structure.

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References

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