The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Comparison of geniposide quantification and antioxidant effect among the various parts of Gardeniae fructus

치자(梔子)의 부위별 Geniposide정량 및 항산화효능비교

  • Kim, Seung-Taik (Department of Herbology, College of Oriental Medicine, Sangji University) ;
  • Lee, Jang-Cheon (Devision of Pharmacology and Basic Korean Medicine, School of Korean Medicine, Pusan National University) ;
  • Lee, Boo-Kyun (Devision of Pharmacology and Basic Korean Medicine, School of Korean Medicine, Pusan National University) ;
  • Lee, Keum-San (Department of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Lyu, Ji-Hyo (Department of Biochemistry & Molecular Biology, Aging-Associated Vascular Disease Research Center, College of Medicine, Yeungnam University) ;
  • Lee, Young-Cheol (Department of Herbology, College of Oriental Medicine, Sangji University)
  • 김승택 (상지대학교 한의과대학 본초학교실) ;
  • 이장천 (부산대학교 한의학전문대학원) ;
  • 이부균 (부산대학교 한의학전문대학원) ;
  • 이금산 (원광대학교 한의과대학 본초학교실) ;
  • 류지효 (영남대학교 의과대학 생화학.분자생물학교실, 노인성혈관질환연구센터) ;
  • 이영철 (상지대학교 한의과대학 본초학교실)
  • Received : 2013.06.19
  • Accepted : 2013.07.23
  • Published : 2013.07.30
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Abstract

Objectives : The objective of the paper was to study cutting method of Gardeniae frutus, that tends to show symptom of separations in a packing, in accordance with the comparison experiment of quantification and antioxidant effect proceed with the intact Gardeniae fructus(Gf), seed of Gardeniae fructus (Gs) and the pericarp of Gardeniae fructus (Gp) separately. Methods : The Gf, Gs, and Gp were extracted using 80% MeOH, followed by quantizing geniposide contained in each group. A MTT assay was conducted and ROS generation and NO production were measured for comparing its antioxidant effect. Results : As a result of quantizing geniposide contained in the Gf, Gp, and Gs, respectively, the geniposide content was shown to be the highest in the Gs. MTT assay showed that no cytotoxicity was observed in the groups treated with Gf, Gp, and Gs, respectively, at a dose of 500 ${\mu}g/ml$. The ROS generation was shown to have more significantly decreased in the group pretreated with Gf 500 ${\mu}g/ml$ than in the group treated with LPS. The NO level was shown to have more significantly decreased in the group pretreated with Gp 500 ${\mu}g/ml$ than in the group treated with LPS. Conclusion : As the geniposide content and antioxidant effect of Gf varies according to its each part, it is recommended that Gf should be distributed as an intact form other than segregation in packing.

Keywords

References

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