The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory and Anti-Pruritonic Effects of WSY-1075 composited with Medicinal Plants on the Activated Rat Peritoneal Mast cells and Mouse Pruritus

활성화된 렛트 비만세포와 마우스 소양증에 대한 한약재로 조성된 WSY-1075의 항염증 및 항소양 효과

  • Hwang, Sung Yeoun (Department of Oriental Medical Prescription, Woosuk University) ;
  • Lee, Seung Ho (Department of Oriental Medical Prescription, Woosuk University) ;
  • Lee, Chia Wei (Department of Oriental Medical Prescription, Woosuk University) ;
  • Kim, Jang Ho (Honam institute for Regional Program) ;
  • Jang, Seon Il (Department of Healthcare & Science, Jeonju University) ;
  • Kim, An Na (Department of Oriental Medical Prescription, Woosuk University) ;
  • Kim, Hong Jun (Department of Oriental Medical Prescription, Woosuk University)
  • 황성연 (우석대학교 한의과대학 방제학교실) ;
  • 이승호 (우석대학교 한의과대학 방제학교실) ;
  • 이가위 (우석대학교 한의과대학 방제학교실) ;
  • 김장호 (호남지역사업평가원) ;
  • 장선일 (전주대학교 보건관리학과) ;
  • 김안나 (우석대학교 한의과대학 방제학교실) ;
  • 김홍준 (우석대학교 한의과대학 방제학교실)
  • Received : 2013.06.20
  • Accepted : 2013.07.18
  • Published : 2013.07.30
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Abstract

Objectives : This study was to evaluate the anti-inflammatory and anti-pruritic effects of WSY-1075 composited with Corni Fructus, Angelica gigantis Radix, Lycii Fructus, Ginseng Radix, Cervi parvum Cornu and Cinnamomi Cortex in rat peritoneal mast cells (RPMCs) and scratching mouse model. Methods : WSY-1075 was prepared by extracting with 30% ethanol. In the present study, we investigated the effect of WSY-1075 on the production of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$) and histamine in rat peritoneal mast cells (RPMCs) activated with phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187, and on the scratching behavior in mice treated with pruriogens. Results : WSY-1075 was not cytotoxic effect in used all concentration. PMA plus A23187 treatment significantly increased TNF-${\alpha}$, IL-$1{\beta}$ and IL-6 production compared with media control in RPMCs. However, TNF-${\alpha}$, $IL1{\beta}$ and IL-6 production increased by PMA plus A23187 treatment were significantly inhibited by WSY-1075 (200 ${\mu}g/mL$ and 400 ${\mu}g/mL$). WSY-1075 also inhibited the histamine release from RPMCs stimulated by compound 48/80, which promotes histamine release. Moreover, WSY-1075 administration had an inhibitory effects on the scratching behavior induced by pruritogen (compound 48/80, histamine, serotonin and substence P) in ICR mice. Conclusion : These results suggest that WSY-1075 administration (200 mg/kg or 400 mg/kg) has the anti-inflammatory and anti-pruritic effects on the activated rat peritoneal mast cell and mouse pruritus. WSY-1075 has a potential use as a composition of medicinal plants for treatment against inflammation- and pruritus-related disease.

Keywords

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