Journal of the Korea Institute of Military Science and Technology (한국군사과학기술학회지)

The Korea Institute of Military Science and Technology (한국군사과학기술학회)
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Decontamination Condition of Geobacillus Stearothermophilus Spore on the Surface of Various Coupons using Hydrogen Peroxide Vapor

과산화수소 증기를 이용한 다양한 쿠폰 표면의 Geobacillus Stearothermophilus 아포 제독조건

  • Received : 2013.05.18
  • Accepted : 2013.07.26
  • Published : 2013.08.05
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Abstract

Biological decontamination means the removal of microorganisms from the inanimate object such as building or equipment. In this study, hydrogen peroxide vapor efficacy test using VHP 1000ED system(Steris LifeSciences) were conducted for G. stearothermophilus spore with agent materials(aluminum, stainless steel, poly-carbonate, viton, silicone, kapton and glass). Total recovered spores exposed to hydrogen peroxide vapor(1.0 g/min) during 7, 15, 30, 60 min were calculated. As a result, all agent materials were totally decontaminated within 60 min at 1.0 g/min concentration with 35% hydrogen peroxide vapor. Finally, we could confirmed that hydrogen peroxide vapor possess sporicidal capacity of G. stearothermophilus and found the optimum decontamination conditions with VHP1000ED system.

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References

  1. M. W. Calfee, S. P. Ryan, J. P. Wood, L. Mickelsen, C. Kempter, L. Miller, M. Colby, A. Touati, M. Clayton, N. Griffin-Gatchalian, S. McDonald, and R. Delafield, "Laboratory Evaluation of Large-Scale Decontamination Approaches", J Appl Microbiol, Vol. 112, pp. 874-882, 2012. https://doi.org/10.1111/j.1365-2672.2012.05259.x
  2. N. A. Klapes, and D. Vesley, "Vapor-Phase Hydrogen Peroxide as a Surface Decontaminant and Sterilant", Appl Environ Microbiol, Vol. 56, pp. 503-506, 1990.
  3. T. L. Buhr, A. A. Young, Z. A. Minter, C. M. Wells, D. C. McPherson, C. L. Hooban, C. A. Johnson, E. J. Prokop, and J. R. Crigler, "Test Method Development to Evaluate Hot, Humid Air Decontamination of Materials Contaminated with Bacillus Anthracis Sterne and B. Thuringiensis Al Hakam Spores", J Appl Microbiol, Vol. 113, pp. 1037-1051, 2012. https://doi.org/10.1111/j.1365-2672.2012.05423.x
  4. C. Franco, and N. Bouri, "Environmental Decontamination Following a Large-Scale Bioterrorism Attack : Federal Progress and Remaining Gaps", Biosecure Bioterror, Vol. 8, pp. 107-117, 2010. https://doi.org/10.1089/bsp.2010.0009
  5. G. S. Graham, and J. R. Rickloff, "Development of VHP Sterilization Technology", J Healthc Mater Manage, Vol. 10, No. 54, pp 56-58, 1992.
  6. R. A. Heckert, M. Best, L. T. Jordan, G. C. Dulac, D. L. Eddington, and W. G. Sterritt, "Efficacy of Vaporized Hydrogen Peroxide against Exotic Animal Viruses", Appl Environ Microbiol, Vol. 63, pp. 3916 -3918, 1997.
  7. J. W. Johnson, J. F. Arnold, S. L. Nail, and E. Renzi, "Vaporized Hydrogen Peroxide Sterilization of Freeze Dryers", J Parenter Sci Technol, Vol. 46, pp. 215-225, 1992.
  8. M. Kokubo, T. Inoue, and J. Akers, "Resistance of Common Environmental Spores of the Genus Bacillus to Vapor Hydrogen Peroxide", PDA J Pharm Sci Technol, Vol. 52, pp. 228-231, 1998.
  9. J. Krause, G. McDonnell, and H. Riedesel, "Biodecontamination of Animal Rooms and Heat-Sensitive Equipment with Vaporized Hydrogen Peroxide", Contemp Top Lab Anim Sci, Vol. 40, pp. 18-21, 2001.
  10. J. A. Otter, and S. Yezli, "Are Commercially Available Geobacillus Stearothermophilus Biological Indicators an Appropriate Standard for Hydrogen Peroxide Vapour Systems in Hospitals?", J Hosp Infect, Vol. 80, pp. 272-273, 2012.
  11. J. V. Rogers, C. L. Sabourin, Y. W. Choi, W. R. Richter, D. C. Rudnicki, K. B. Riggs, M. L. Taylor, and J. Chang, "Decontamination Assessment of Bacillus Anthracis, Bacillus Subtilis, and Geobacillus Stearothermophilus Spores on Indoor Surfaces Using a Hydrogen Peroxide Gas Generator", J Appl Microbiol, Vol. 99, pp. 739-748, 2005. https://doi.org/10.1111/j.1365-2672.2005.02686.x
  12. L. Teri, D. B. Mark, P. Jerry, "Biological-warfare Agent Decontamination Efficacy Testing : Largescale Chamber mVHP Decontamination System Evaluation for Biological Contamination", Edgewood Chemical Biological Center, No. ECBC-TR-521, 2007.
  13. L. Teri, D. B. Mark, P. Jerry, "Evaluation of the STERIS Sensitive Equipment Decontamination (SED) Apparatus on a 463L Pallet", Edgewood Chemical Biological Center, No. ECBC-TR-526, 2007.
  14. K. R. Vipin, W. Lalena, S. S. Lisa, P. Jerry, "Surface Sampling-based Decontamination Studies and Protocol for Determining Sporicidal Efficacy of Gaseous Fumigants on Military-relevant Surfaces", Edgewood Chemical Biological Center, No. ECBCTR-595, 2008.