Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of High Pressure Treatment on Antioxidant Compounds and Activity of Germinated Rough Rice (Oryza sativa L.)

고압처리가 발아벼의 항산화 성분과 활성에 미치는 영향

  • Kim, Min Young (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Sang Hoon (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jang, Gwi Young (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Park, Hye Jin (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Meishan, Li (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kim, Shinje (Center for Fungi and Plant Genome Research, FnP Corp.) ;
  • Lee, Youn Ri (Dept. of Food and Nutrition, Daejeon Health Sciences College) ;
  • Lee, Junsoo (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • Received : 2013.07.22
  • Accepted : 2013.08.23
  • Published : 2013.11.30
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Abstract

This study is investigated to evaluate the enhancement of antioxidant compound and activity of rough rice with different germination periods and high pressure treatment. The subject was germinated at $37^{\circ}C$ for 6 days (HP0), and then the germinated rough rice were subjected to 30 MPa for 24 hr (HP24) and 48 hr (HP48), respectively. HP0, HP24 and HP48 samples were prepared and extracted with 80% ethanol. The highest total polyphenol contents (5.15 mg/g) occurred in treating at HP48 after germination for 5 days. The total phenolic acid contents including gallic acid, chlorogenic acid, catechin, ${\rho}$-coumaric acid, ferulic acid, salicylic acid, naringin, myricetin, trans-cinnamic acid, naringenin and kaempferol increased from $37.26{\sim}204.32{\mu}g/g$ at HP0 to $77.29{\sim}283.05{\mu}g/g$ at HP48. In antioxidant activity analyses, HP48 extracts showed higher values in ABTS and DPPH radical scavenging activity, reducing power, and $Fe^{2+}$ iron chelating effect than those of the HP24 and HP0 extracts. These results suggest that the combined treatment of high pressure treatment and germination process efficiently enhanced antioxidant compound and activity of rough rice.

본 연구에서는 발아기간 및 고압처리 시간에 따른 항산화 성분 및 항산화 활성을 조사함으로써 발아벼의 항산화 활성에 미치는 고압처리공정의 효과에 대해 연구하였다. 발아기간은 6일까지로 하였고, 기간별로 발아된 벼는 30 MPa의 압력 하에서 24시간 및 48시간 동안 처리하였다. 총 폴리페놀함량은 48시간 고압처리를 실시한 6일차 발아벼가 5.15 mg/g으로 고압처리를 실시하지 않은 6일차 발아벼의 1.11에 비하여 증가하였으며, 15종의 페놀산 중 gallic acid 외 6종의 페놀산과 총 페놀산함량이 발아기간 및 고압처리 시간이 증가함에 따라 유의적으로 증가하였다. ABTS 라디칼 소거능과 DPPH 라디칼 소거능은 대조구의 경우 발아 4일차에서 최대 값을 나타내었으며, 고압처리 24시간 처리구와 48시간 처리구 모두 대조구에 비해 높았다. 환원력과 이온킬레이팅 효과는 총 폴리페놀, 페놀산 함량과 유사하게 발아기간 및 고압처리시간이 증가함에 따라 증가하였다. 본 연구결과 발아와 가압을 병행처리 하였을 때 일반벼 및 발아벼에 비해 항산화 성분 및 항산화 활성이 증가함을 알 수 있었으며, 발아벼의 기능성을 증대시키기 위하여 고압처리공정의 적용이 효과적이라고 판단된다.

Keywords

References

  1. Lim CS, Li CY, Kim YM, Lee WY, Rhee HI. 2005. The inhibitory effect of Cornus walteri extract against $\alpha$-amylase. J Korean Soc Appl Biol Chem 48: 103-108.
  2. Kang MY, Lee YR, Nam SH. 2003. Characterization of the germinated rices to examine an application potentials as functional rice processed foods. Korean J Food Sci Technol 35: 696-701.
  3. Kim LS, Son YK, Son JR, Hur HS. 2001. Effect of germination condition and drying methods on physicochemical properties of sprouted brown rice. Korean J Crop Sci 46:221-228.
  4. Saunder RM. 1990. The properties of rice bran as a food stuff. CFW 35: 632-636.
  5. Andreason MF, Christensen LP, Meyer AS, Hansen A. 1999. Release of hydroxycinnamic and hydroxybenzoic acids in rye by commercial plant cell wall degrading enzyme preparations. J Sci Food Agric 79: 411-413. https://doi.org/10.1002/(SICI)1097-0010(19990301)79:3<411::AID-JSFA264>3.0.CO;2-X
  6. Bartnick M, Szafranska I. 1987. Changes in phytate content and phytase activity during the germination of some cereals. J Cereal Sci 5: 23-28. https://doi.org/10.1016/S0733-5210(87)80005-X
  7. Lee YR, Kim JY, Woo KS, Hwang IG, Kim KH, Kim KJ, Kim JH, Jeong HS. 2007. Changes in the chemical and functional components of Korean rough rice before and after germination. Food Sci Biotechnol 16: 1006-1010.
  8. Ko JY, Song SB, Lee JS, Kang JR, Seo MC, Oh BG, Kwak DY, Nam MH, Jeong HS, Woo KS. 2011. Changes in chemical components of foxtail millet, proso millet, and sorghum with germination. J Korean Soc Food Sci Nutr 40: 1128-1135. https://doi.org/10.3746/jkfn.2011.40.8.1128
  9. Lee EH, Kim CJ. 2008. Nutritional changes of buckwheat during germination. Korean J Food Culture 23: 121-129.
  10. Ghung DS, Kim HK. 1998. Changes of protein and lipid composition during germination of Perilla frutescens seeds. Korean J Life Science 8: 318-325.
  11. Kim JS, Kim JG, Kim WJ. 2004. Changes in isoflavone and oligosaccharides of soybeans during germination. Korean J Food Sci Technol 36: 294-298.
  12. Kim HY, Hwang IG, Kim TM, Park DS, Kim JK, Kim DJ, Lee YR, Lee JS, Jeong HS. 2011. Changes in chemical composition of rough rice (Oryza sativa L.) according to germination period. J Korean Soc Food Sci Nutr 40: 1265-1270. https://doi.org/10.3746/jkfn.2011.40.9.1265
  13. Kim HY, Hwang IG, Kim TM, Park DS, Kim JH, Kim DJ, Lee JS, Jeong HS. 2011. Antioxidant and angiotensin converting enzyme I inhibitory activity on different parts of germinated rough rice. J Korean Soc Food Sci Nutr 40:775-780. https://doi.org/10.3746/jkfn.2011.40.6.775
  14. Kim HY, Hwang IG, Joung EM, Kim TM, Kim DJ, Park DS, JS Lee, Jeong HS. 2010. Antiproliferation effects of germinated-Korean rough rice extract on human cancer cells. J Korean Soc Food Sci Nutr 39: 325-330. https://doi.org/10.3746/jkfn.2010.39.3.325
  15. Kwon SM, Kim CM, Kim YH. 2007. Biological characteristics of instant rice treated with high hydrostatic pressure. Food Science and Industry 40(3): 31-35.
  16. San Martin MF, Barbosa-Cánovas GV, Swanson BG. 2002. Food processing by high hydrostatic pressure. Crit Rev Food Sci Nutr 42: 627-645. https://doi.org/10.1080/20024091054274
  17. Kim SO, Park CW, Moom S, Lee HA, Kim B, Lee DU, Lee JH, Park J. 2007. Effects of high-hydrostatic pressure on ginsenoside concentrations in Korean red ginseng. Food Sci Biotechnol 16: 848-853.
  18. Corrales M, Toepfl S, Butz P, Knorr D, Tauscher B. 2008. Extraction of anthocyanins from grape by-products assisted by ultrasonics, high hydrostatic pressure or pulsed electric fields: A comparison. Innovative Food Sci Emerging Technol 9: 85-91. https://doi.org/10.1016/j.ifset.2007.06.002
  19. Kim D, Han GD. 2012. High Hydrostatic pressure treatment combined with enzymes increase the extractability and bioactivity of fermented rice bran. Innovative Food Sci Emerging Technol 16: 191-197. https://doi.org/10.1016/j.ifset.2012.05.014
  20. Park SJ, Park DS, Lee SB, He X, Ahn JH, Woon WB, Lee HY. 2010. Enhancement of antioxidant activities of Codonopsis lanceolata and fermented Codonopsis lanceolata by ultra high pressure extraction. J Korean Soc Food Sci Nutr 39: 1898-1902. https://doi.org/10.3746/jkfn.2010.39.12.1898
  21. Northrop DB. 2002. Effects of high pressure on enzymatic activity. Biochim Biophys Acta 1595: 71-79. https://doi.org/10.1016/S0167-4838(01)00335-1
  22. Real V, Alfaia AJ, Calado ART, Ribeiro MHL. 2007. High pressure-temperature effects on enxymatic activity: Naringin bioconversion. Food Chem 102: 565-570. https://doi.org/10.1016/j.foodchem.2006.05.033
  23. Liu W, Liu J, Liu C, Zhong Y, Liu W, Wan J. 2009. Activation and conformational changes of mushroom polyphenoloxidase by high pressure microfluidization treatment. Innovative Food Sci Emerging Technol 10: 142-147. https://doi.org/10.1016/j.ifset.2008.11.009
  24. Kim D, Fan JP, Chung HC, Han GD. 2010. Changes in extractability and antioxidant activity of Jerusalem artichoke (Helianthus tuberosus L.) tubers by various high hydrostatic pressure treatments. Food Sci Biotechnol 19: 1365-1371. https://doi.org/10.1007/s10068-010-0194-8
  25. Dewanto V, Wu X, Liu RH. 2002. Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50: 4959-4964. https://doi.org/10.1021/jf0255937
  26. Seo MC, Ko JY, Song SB, Lee JS, Kang JR, Kwak DY, Oh BG, Yoon YN, Nam MH, Jeong HS, Woo KS. 2011. Antioxidant compounds and activities of foxtail millet, 93 proso millet and sorghum with different pulverizing methods. J Korean Soc Food Sci Nutr 40: 790-797. https://doi.org/10.3746/jkfn.2011.40.6.790
  27. Jung KH, Hong HD, Cho CW, Lee MY, Choi UK, Kim YC. 2012. Phenolic acid composition and antioxidative activity of red ginseng prepared by high temperature and high pressure process. Korean J Food & Nutr 25: 827-832. https://doi.org/10.9799/ksfan.2012.25.4.827
  28. Choi Y, Lee SM, Chun J, Lee HB, Lee J. 2006. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of shiitake (Lentinus edodes) mushroom. Food Chem 99: 381-387. https://doi.org/10.1016/j.foodchem.2005.08.004
  29. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS. 2006. Change of physicochemical characteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J Food Sci Technol 38: 342-347.
  30. Mau JL, Lin HC, Song SF. 2002. Antioxidant properties of several specialty mushrooms. Food Res Int 35: 519-526. https://doi.org/10.1016/S0963-9969(01)00150-8
  31. Singh N, Rajini PS. 2004. Free radical scavenging activity of an aqueous extract of potato pell. Food Chem 85: 611-616. https://doi.org/10.1016/j.foodchem.2003.07.003
  32. Kim HY, Lee SH, Hwang IG, Jeong HS. 2012. Antioxidant activity and anticancer effects of rough rice (Oryza sativa L.) by germination periods. J Korean Soc Food Sci Nutr 41: 14-19. https://doi.org/10.3746/jkfn.2012.41.1.014
  33. Tian S, Nakamura K, Cui T, Kayahara H. 2005. High-performance liquid chromatographic determination of phenolic compounds in rice. J Chromatogr A 1063: 121-128. https://doi.org/10.1016/j.chroma.2004.11.075
  34. Rice-Evans C, Miller N, Paganga G. 1997. Antioxidant properties of phenolic compounds. Trends in Plant Sci 2:152-159. https://doi.org/10.1016/S1360-1385(97)01018-2
  35. Middleton E, Kandaswami C. 1994. Potential health-promoting properties of citrus flavonoids. Food Technol 48:115-119.
  36. Yang F, Basu TK, Ooraikul B. 2001. Studies on germination conditions and antioxidant contents of wheat grain. Int J Food Sci Nutr 52: 319-330. https://doi.org/10.1080/09637480120057567
  37. Hung PV, Hatcher DW, Barker W. 2011. Phenolic acid composition of sprouted wheats by ultra-performance liquid chromatography (UPLC) and their antioxidant activities. Food Chem 126: 1896-1901. https://doi.org/10.1016/j.foodchem.2010.12.015
  38. Xi J, Shen D, Zhao S, Lu B, Li Y, Zhang R. 2009. Characterization of polyphenols from green tea leaves using a high hydrostatic pressure extraction. Int J Pharm 382: 139-143. https://doi.org/10.1016/j.ijpharm.2009.08.023
  39. Kang MY, Kim S, Koh HJ, Nam SH. 2004. Antioxidant activity of ethanolic extract from germinated giant embryonic rices. J Korean Soc Appl Biol Chem 47: 293-299.
  40. Xi J, Shen D, Li Y, Zhang R. 2011. Ultrahigh pressure extraction as a tool to improve the antioxidant activities of green tea extracts. Food Res Int 44: 2783-2787. https://doi.org/10.1016/j.foodres.2011.06.001
  41. Gordon MF. 1990. The mechanism of antioxidant action in vitro. In Food Antioxidants. Hudson BJF, ed. Elsevier Applied Science, London, UK. p 1-18.
  42. Lee YR, Woo KS, Kim JY, Son JR, Jeong HS. 2007. Antioxidant activities of ethanol extracts from germinated specialty rough rice. Food Sci Biotechnol 16: 765-770.
  43. Hsu CL, Chen W, Weng YM, Tseng CY. 2003. Chemical composition, physical properties, and antioxidant activities of yam flours as affected by different drying methods. Food Chem 83: 85-92. https://doi.org/10.1016/S0308-8146(03)00053-0
  44. Elmastas M, Gulcin I, Isildak O, Kufrevioglu OI, Ibaoglu K, Aboul-Enein HY. 2006. Radical scavenging activity and antioxidant capacity of bay leaf extracts. J Iran Chem Soc 3: 258-266. https://doi.org/10.1007/BF03247217

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