Journal of Oral Medicine and Pain

Korean Academy of Orofacial Pain and Oral Medicine (대한안면통증구강내과학회)
권호 표지
DOI QR코드

DOI QR Code

Interactions between Hyaluronic Acid, Lysozyme, Peroxidase, and Glucose Oxidase in Enzymatic Activities at Low pH

  • Kim, Bum-Soo (Department of Oral Medicine and Oral Diagnosis, School of Dentistry, Seoul National University) ;
  • Kim, Yoon-Young (Department of Oral Medicine and Oral Diagnosis, School of Dentistry, Seoul National University) ;
  • Chang, Ji-Youn (Department of Oral Medicine and Oral Diagnosis, School of Dentistry, Seoul National University) ;
  • Kho, Hong-Seop (Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University)
  • Received : 2014.08.01
  • Accepted : 2014.08.27
  • Published : 2014.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: Many substances in saliva or oral health care products interact with each other. The aim of this study was to investigate interactions between hyaluronic acid (HA), lysozyme, peroxidase, and glucose oxidase (GO) in enzymatic activities at low pH levels. Methods: HA (0.5 mg/mL), hen egg-white lysozyme (HEWL, $30{\mu}g/mL$), bovine lactoperoxidase (bLPO, $25{\mu}g/mL$), and GO ($50{\mu}g/mL$) were used. The influences of HA, bLPO, and GO on HEWL activity were determined by measuring the turbidity of a Micrococcus lysodeikticus suspension. The influences of HA and HEWL on bLPO activity were determined by the NbsSCN assay, measuring the rate of oxidation of 5-thio-2-nitrobenzoic acid (Nbs) to 5,5'-dithiobis(2-nitrobenzoic acid) $(Nbs)_2$. The influences of HA and HEWL on GO activity were determined by measuring oxidized o-dianisidine production. All experiments were performed at pH 4, 5, and 6. Results: HA and GO did not affect the enzymatic activity of HEWL at pH 4, 5, and 6. bLPO enhanced the enzymatic activity of HEWL at pH 5 (p<0.05) and pH 6 (p<0.05) significantly. The enzymatic activity of bLPO was not affected by HA and HEWL at pH 4, 5, and 6. HA and HEWL did not affect the enzymatic activity of the GO at pH 4, 5, and 6. Conclusions: Peroxidase enhances lysozyme activity at low pH, otherwise there were no significant interactions in enzymatic activities between HA, lysozyme, peroxidase, and GO at low pH levels.

Keywords

References

  1. Almond A. Hyaluronan. Cell Mol Life Sci 2007;64:1591-1596. https://doi.org/10.1007/s00018-007-7032-z
  2. Park MS, Chang JY, Kang JH, Park KP, Kho HS. Rheological properties of hyaluronic acid and its effects on salivary enzymes and candida. Oral Dis 2010;16:382-387. https://doi.org/10.1111/j.1601-0825.2009.01650.x
  3. Sakai A, Akifusa S, Itano N, et al. Potential role of high molecular weight hyaluronan in the anti-Candida activity of human oral epithelial cells. Med Mycol 2007;45:73-79. https://doi.org/10.1080/13693780601039607
  4. Chen WY, Abatangelo G. Functions of hyaluronan in wound repair. Wound Repair Regen 1999;7:79-89. https://doi.org/10.1046/j.1524-475X.1999.00079.x
  5. Kang JH, Kim YY, Chang JY, Kho HS. Influences of hyaluronic acid on the anticandidal activities of lysozyme and the peroxidase system. Oral Dis 2011;17:577-583. https://doi.org/10.1111/j.1601-0825.2011.01807.x
  6. Higuchi Y, Ansai T, Awano S, et al. Salivary levels of hyaluronic acid in female patients with dry mouth compared with age-matched controls: a pilot study. Biomed Res 2009;30:63-68. https://doi.org/10.2220/biomedres.30.63
  7. Tenovuo J. Clinical applications of antimicrobial host proteins lactoperoxidase, lysozyme and lactoferrin in xerostomia: efficacy and safety. Oral Dis 2002;8:23-29. https://doi.org/10.1034/j.1601-0825.2002.1o781.x
  8. Laible NJ, Germaine GR. Bactericidal activity of human lysozyme, muramidase-inactive lysozyme, and cationic polypeptides against Streptococcus sanguis and Streptococcus faecalis: inhibition by chitin oligosaccharides. Infect Immun 1985;48:720-728.
  9. Ibrahim HR, Matsuzaki T, Aoki T. Genetic evidence that antibacterial activity of lysozyme is independent of its catalytic function. FEBS Lett 2001;506:27-32. https://doi.org/10.1016/S0014-5793(01)02872-1
  10. Ihalin R, Loimaranta V, Tenovuo J. Origin, structure, and biological activities of peroxidases in human saliva. Arch Biochem Biophys 2006;445:261-268. https://doi.org/10.1016/j.abb.2005.07.004
  11. Hannig C, Hannig M, Attin T. Enzymes in the acquired enamel pellicle. Eur J Oral Sci 2005;113:2-13. https://doi.org/10.1111/j.1600-0722.2004.00180.x
  12. Tenovuo J, Lumikari M, Soukka T. Salivary lysozyme, lactoferrin and peroxidases: antibacterial effects on cariogenic bacteria and clinical applications in preventive dentistry. Proc Finn Dent Soc 1991;87:197-208.
  13. Tenovuo J. Antimicrobial function of human saliva--how important is it for oral health? Acta Odontol Scand 1998;56:250-256. https://doi.org/10.1080/000163598428400
  14. Lenander-Lumikari M, Loimaranta V. Saliva and dental caries. Adv Dent Res 2000;14:40-47. https://doi.org/10.1177/08959374000140010601
  15. Soukka T, Lumikari M, Tenovuo J. Combined bactericidal effect of human lactoferrin and lysozyme against Streptococcus mutans serotype c. Microbial Ecol Health Dis 1991;4:259-264. https://doi.org/10.3109/08910609109140275
  16. Murakami Y, Nagata H, Amano A, et al. Inhibitory effects of human salivary histatins and lysozyme on coaggregation between Porphyromonas gingivalis and Streptococcus mitis. Infect Immun 1991;59:3284-3286.
  17. Lee JY, Kim YY, Chang JY, Park MS, Kho HS. The effects of peroxidase on the enzymatic and candidacidal activities of lysozyme. Arch Oral Biol 2010;55:607-612. https://doi.org/10.1016/j.archoralbio.2010.06.001
  18. Lenander-Lumikari M, Mansson-Rahemtulla B, Rahemtulla F. Lysozyme enhances the inhibitory effects of the peroxidase system on glucose metabolism of Streptococcus mutans. J Dent Res 1992;71:484-490. https://doi.org/10.1177/00220345920710031201
  19. Cho MA, Kim YY, Chang JY, Kho HS. Interactions between hyaluronic acid, lysozyme, and the glucose oxidase-mediated lactoperoxidase system in enzymatic and candidacidal activities. Arch Oral Biol 2013;58:1349-1356. https://doi.org/10.1016/j.archoralbio.2013.06.015
  20. Tenovuo J, Moldoveanu Z, Mestecky J, Pruitt KM, Rahemtulla BM. Interaction of specific and innate factors of immunity: IgA enhances the antimicrobial effect of the lactoperoxidase system against Streptococcus mutans. J Immunol 1982;128:726-731.
  21. Lassiter MO, Newsome AL, Sams LD, Arnold RR. Characterization of lactoferrin interaction with Streptococcus mutans. J Dent Res 1987;66:480-485. https://doi.org/10.1177/00220345870660021601
  22. Soukka T, Lumikari M, Tenovuo J. Combined inhibitory effect of lactoferrin and lactoperoxidase system on the viability of Streptococcus mutans, serotype c. Scand J Dent Res 1991;99:390-396.
  23. Bennick A, Cannon M. Quantitative study of the interaction of salivary acidic proline-rich proteins with hydroxyapatite. Caries Res 1978;12:159-169. https://doi.org/10.1159/000260326
  24. Grossowicz N, Ariel M. Methods for determination of lysozyme activity. Methods Biochem Anal 1983;29:435-446. https://doi.org/10.1002/9780470110492.ch8
  25. Mansson-Rahemtulla B, Baldone DC, Pruitt KM, Rahemtulla F. Specific assays for peroxidases in human saliva. Arch Oral Biol 1986;31:661-668. https://doi.org/10.1016/0003-9969(86)90095-6
  26. Luke GA, Gough H, Beeley JA, Geddes DA. Human salivary sugar clearance after sugar rinses and intake of foodstuffs. Caries Res 1999;33:123-129. https://doi.org/10.1159/000016505
  27. Turner MD, Ship JA. Dry mouth and its effects on the oral health of elderly people. J Am Dent Assoc 2007;138 Suppl:15S-20S. https://doi.org/10.14219/jada.archive.2007.0358
  28. Avila JL, Convit J. Inhibition of leucocytic lysosomal enzymes by glycosaminoglycans in vitro. Biochem J 1975;152:57-64. https://doi.org/10.1042/bj1520057
  29. Baker MS, Green SP, Lowther DA. Changes in the viscosity of hyaluronic acid after exposure to a myeloperoxidase-derived oxidant. Arthritis Rheum 1989;32:461-467. https://doi.org/10.1002/anr.1780320416
  30. Lindvall S, Rydell G. Influence of various compounds on the degradation of hyaluronic acid by a myeloperoxidase system. Chem Biol Interact 1994;90:1-12. https://doi.org/10.1016/0009-2797(94)90106-6

Cited by

  1. Biomimetic Hyaluronic Acid-Lysozyme Composite Coating on AZ31 Mg Alloy with Combined Antibacterial and Osteoinductive Activities vol.3, pp.12, 2014, https://doi.org/10.1021/acsbiomaterials.7b00527