Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Study on the anti-allergy activity of mushrooms in IgE-sensitized RBL-2H3 cells

IgE로 감작된 RBL-2H3 세포에서 버섯자원의 항알레르기 활성 탐색

  • Lee, Seung-Eun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Noh, Hyung-Jun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Choi, Jehun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Kim, Geum-Sook (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Lee, Dae-Young (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Kim, Seung-Yu (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
  • 이승은 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 노형준 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 최재훈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김금숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이대영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김승유 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2014.11.10
  • Accepted : 2014.12.11
  • Published : 2014.12.31
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Abstract

For evaluating the anti-allergy activity of mushrooms, forty two mushroom extracts were studied their inhibitory activities on the IgE-mediated degranulation in rat basophilic leukemia cell line (RBL-2H3 cell). Effects of mushroom extracts on the release of interleukin-4 (IL-4) and beta-hexosaminidase, and the cell viability of the IgE-sensitized were measured. From the analysis, five mushroom extracts such as the water extract from Flammulina velutipes (Curt.:Fr.) Sing showed the suppressive activities on IL-4 release as 20%. Eight extracts including the water extract of Ganoserma lucidum showed the suppressive activities on ${\beta}$-hexosaminidase release as 20%. Almost all of the extracts stimulated the proliferation of RBL-2H3 cell. The water extracts of Flammulina velutipes and Phellinus linteus were examined against the inhibitory activity in the production of IL-4 and ${\beta}$-hexosaminidase. Additionally, the extracts from Ganoserma lucidum, Isaria japonica, Phellinus linteus and Pleurotus ostreatus inhibited dose-dependently on ${\beta}$-hexosaminidase production. In conclusion, the result suggests that the mushrooms with the potent inhibitory efficacies on the degranulation of the mast cell would be candidate resources for the anti-allergy resources, and thus need to study for their utilization.

본 연구에서는 버섯추출물 (42개)의 in vitro 항알레르기 효능탐색을 위해 랫드비만세포 (RBL-2H3 cell)에서 면역글로불린 (IgE)가 매개한 탈과립에 대한 저해 효과를 실험하였다. 이를 위해 anti-DNP IgE 및 DNP-HSA에 의해 알레르기반응이 유발된 랫드비만세포에서 버섯 추출물의 IL-4과 ${\beta}$-hexosaminidase 분비량에 대한 저해활성과 세포생존에 대한 영향이 분석되었다. 실험결과, IL-4 분비에 대해서는 팽이버섯 물추출물 등 5개의 버섯 추출물이 20% 이상의 우수한 저해효과를 나타내었으며, ${\beta}$-hexosaminidase 분비에 대해서는 영지버섯의 물추출물 등 8개의 버섯 추출물이 20% 이상의 비교적 우수한 저해활성을 나타내었다. 세포증식에 대해서는 잎새버섯의 물추출물등 대부분의 버섯 추출물이 우수한 세포증식효과를 나타내었다. 팽이버섯의 물추출물과 상황버섯의 물추출물은 ${\beta}$-hexosaminidase 및 IL-4 분비에 대해 모두 비교적 우수한 저해효과를 나타내었다. 추가로 2, 10, 50 ug/ml에서 실험된 영지버섯, 편각영지버섯, 눈꽃동충하초, 상황버섯 그리고 느타리버섯의 물추출물들은 ${\beta}$-hexosaminidase의 분비량을 농도-의존적으로 감소시켰다. 이상의 결과를 살펴볼때, 이들 in vitro 항알레르기 효과를 나타낸 버섯 추출물들은 추가실험을 통해 항알레르기 소재로의 활용성 검토가 필요하다고 사료되었다.

Keywords

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